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  • 1
    Electronic Resource
    Electronic Resource
    Densitometer with tapered optical fibers (1984)
    s.l. : American Chemical Society
    Analytical chemistry 56 (1984), S. 305-307 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00266a046
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  • 2
    Electronic Resource
    Electronic Resource
    The controls of late dicot embryogenesis and early germination (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During seed formation, the embryo appears to be germinable as soon as cell division is completed; however, it continues development on the plant. This review describes the stages of development after cell division and provides a summary of important observations and recent use of molecular markers as they apply to the regulation of dicot seed formation. Genetic evidence suggests that abscisic acid may help initiate late embryogenesis, although no evidence firmly establishes that abscisic acid controls any other aspect of late dicot development. Previous studies utilizing cultured embryos have implicated abscisic acid and water potential as endogenous promoters of late embryogenesis and inhibitors of germination. However, these embryo culture experiments have been misinterpreted. The experiments show that both immature and mature embryos respond to environmental water stress by expressing a developmental program that is normally induced in late embryogenesis by abscission of the vascular connection. This postabscission program probably prepares the embryo for its forthcoming desiccation during normal development and is predicted to be important in protecting the embryo from water stress during germination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02142.x
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of cDNA clones for NADP-malic enzyme from leaves ofFlaveria: transcript abundance distinguishes C3, C3–C4 and C4 photosynthetic types (1991)
    Springer
    Plant molecular biology 17 (1991), S. 371-383 
    Details
    ISSN: 1573-5028
    Keywords: C4 photosynthesis ; C3–C4 intermediates ; Flaveria ; NADP-malic enzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To study the control of enhanced synthesis of enzymes associated with C4 photosynthesis relative to non-C4 plants, we investigated the expression of NADP-malic enzyme (NADP-ME) in different photosynthetic types ofFlaveria. Complementary DNA clones encoding NADP-ME were constructed using poly(A)+ RNA from leaves ofFlaveria trinervia (C4) andF. linearis (C3–C4) and identified by homology to a cDNA clone (500 bp) encoding NADP-ME from maize (Zea mays L. [39]). The sequence of one clone from each species was determined. TheFlaveria clones were 90% homologous over a 564 nucleotide region encoding the carboxy terminal end of the derived polypeptide; sequence similarity to the maize transcript in this region was 71%. BothFlaveria clones detected a 2/3 kb transcript by hybridization to poly(A)+ RNA from expanding leaves ofF. trinervia, F. linearis andF. pringlei (C3). The level of transcripts paralleled previously observed NADP-ME activity and abundance differences determined in these species, suggesting that control of the expression of NADP-ME in different photosynthetic types is predominantly at the transcriptional/post-transcriptional level. Southern analysis of genomic DNAs fromF. trinervia, F. linearis andF. pringlei indicated a low copy number for this gene in all three species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040632
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  • 4
    Electronic Resource
    Electronic Resource
    Restriction fragment length polymorphisms in diploid and allotetraploid Gossypium: Assigning the late embryogenesis-abundant (Lea) alloalleles in G. hirsutum (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 305-314 
    Details
    ISSN: 1617-4623
    Keywords: Restriction fragment length polymorphisms ; Gossypium hirsutum ; Gene copy number ; Lea, vicilin, and legumin genes ; Tetraploidization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined the copy number of 21 genes in an allotetraploid and several diploid species of cotton by gel and dot blot hybridization with cloned cDNAs. The legumin A, legumin B, and all 18 unique Lea (late embryogenesis-abundant) cDNA sequences isolated from the AD allotetraploid Gossypium hirsutum are present in one copy in A, D, E, and F diploid species and in two copies in G. hirsutum. Gel blot analysis of DNAs digested with EcoRI or BamHI usually detects different sized fragments in A and D diploids. Conservation of these restriction fragment length polymorphisms in G. hirsutum allows most of these fragments to be assigned to their respective subgenomes. Furthermore, both subgenomes in G. hirsutum can be distinguished from those in the interfertile allotetraploid G. barbadense. These results show that physical mapping of both sets of chromosomes in an allotetraploid should be possible by segregation analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330608
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  • 5
    Electronic Resource
    Electronic Resource
    Translation efficiency of Lea mRNAs in cotton embryos: minor changes during embryogenesis and germination (1987)
    Springer
    Plant molecular biology 9 (1987), S. 301-313 
    Details
    ISSN: 1573-5028
    Keywords: Gossypium hirsutum ; embryo culture ; two-dimensional gel electrophoresis ; alloallele expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In earlier studies, only two major patterns of transcript accumulation were seen for 18 late embryogenesis abundant (Lea) gene families in cotton (Gossypium hirsutum L.) during embryogenesis and early germination. Each of these gene families probably comprises two active alloalleles. The two polypeptides encoded by seven of the Lea families can be distinguished, and analysis of their translation in vitro indicated that regulation of the homeologous transcript abundance was similar in each. In the present study, two-dimensional gel electrophoresis of polypeptides synthesized in excised embryos was employed to determine if LEA polypeptide synthesis is regulated at the translational level. The relative in vivo synthesis rate of each of the two polypeptides of 7 Lea families was compared with the relative concentration of their transcripts measured earlier by in vitro translation. For 4 families, the relative translational efficiencies of the homeologous mRNAs do not change during embryogenesis. However, there are changes of 1.5–3-fold in the other 3 families. The translation efficiencies of all transcripts of 9 of the Lea family mRNAs in vivo can be calculated from the fraction of total protein radioactivity incorporated in each LEA polypeptide family and the fractional abundance of Lea family transcripts measured by RNA dot hybridization. Lea mRNAs are found to be translated at near average rates throughout embryogenesis and early germination. These observations suggest that regulation of Lea gene expression at the translational level is minor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014905
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  • 6
    Electronic Resource
    Electronic Resource
    Abscisic acid induction of cloned cotton late embryogenesis-abundant (Lea) mRNAs (1986)
    Springer
    Plant molecular biology 7 (1986), S. 155-170 
    Details
    ISSN: 1573-5028
    Keywords: embryo maturation ; cDNA cloning ; Gossypium hirsutum ; hybrid-arrest translation ; abscisic acid ; embryo culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Earlier studies found that cotton (Gossypium hirsutum L.) cotyledons contain several mRNAs which are more abundant during late embryogenesis than in mid-embryogenesis or early germination. They are here termed ‘Late embryogenesis-abundant’ mRNAs, encoded by Lea loci. Complementary DNA clones for 18 such mRNA sequences, defined at a hybridization criterion of Tm-15°C, were identified in a mature embryo cDNA library by differential cDNA hybridization. At a lower hybridization criterion, some sequence homology was found within several of these cloned Lea mRNA sequences. Each Lea mRNA sequence comprises 0.04–1.3% of mature embryo poly(A)+ mRNA, a level ten-fold to several hundred-fold higher than in young embryo or 24 h seedling poly(A)+ mRNA. Of 18 Lea mRNA sequences examined in cultured young embryos, the level of at least 13 are specifically increased by exogenous abscisic acid (ABA), several to a level near that in normal mature embryos. However, the abundance of several of the sequences does not appear to be significantly modulated by ABA. The LEA polypeptides encoded by 10 Lea mRNA sequences were identified by hybrid-arrested translation. They include most of the late embryogenesis-abundant, ABA-inducible, polypeptides previously identified. Preliminary results suggest that many of the individual Lea mRNA sequences are transcribed from 1–3 genes in each of cotton's two subgenomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021327
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  • 7
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    Unknown
    Densitometer with tapered optical fibers (1984)
    American Chemical Society
    Details
    Publication Date: 1984-02-01
    Print ISSN: 0003-2700
    Electronic ISSN: 1520-6882
    Topics: Chemistry and Pharmacology
    Published by American Chemical Society
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