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  • 1
    Electronic Resource
    Electronic Resource
    Life cycle of the moss,Physcomitrella patens, in culture (1982)
    Springer
    Methods in cell science 7 (1982), S. 19-22 
    Details
    ISSN: 1573-0603
    Keywords: Moss ; Physcomitrella patens ; plant tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method employing tissue culture techniques for growth of mosses is described. This method allows for the completion of the sexual life cycle of the mossPhyscomitrella patens (Hedw.) BSG under controlled conditions in a two month period. The moss system is useful for class demonstration or for research in the areas of development, genetics and plant physiology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01666875
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  • 2
    Electronic Resource
    Electronic Resource
    Mating systems in the xerulaceae (agaricales, basidiomycotina):Flammulina (1999)
    Springer
    Mycoscience 40 (1999), S. 411-426 
    Details
    ISSN: 1618-2545
    Keywords: Basidiomycotina ; mating systems ; Xerulaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recently published taxonomic circumscriptions for taxa withinFlammulina encouraged mating studies to confirm or reject these names or taxa. Three categories of pairing experiments were performed: 1) self-crosses of monokaryon isolates of examplars of various putative taxa; 2) inter-exemplar pairings among exemplar strains; and 3) pairings between examplars and 87 unidentified strains which were grouped on this basis. Mating experiments could distin-guishF. velutipes from other taxa, but not among infraspecific taxa ofF. velutipes (vars.velutipes, lactea, andlupinicola) and monokaryons of all these taxa were partially compatible with those ofF. ononidis. Likewise, isolates ofF. rossica andF. elastica were partially compatible with one another but incompatible with those of other taxa. All other taxa (F. mexicana, F. stratosa, F. populicola, F. fennae) appeared to be genetically isolated. Low levels of interspecific hybridization betweenF. velutipes andF. populicola, and betweenF. velutipes andF. rossica/elastica were also noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02464396
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  • 3
    Electronic Resource
    Electronic Resource
    The consensus land plant chloroplast gene order is present, with two alterations, in the moss Physcomitrella patens (1987)
    Springer
    Molecular genetics and genomics 208 (1987), S. 335-341 
    Details
    ISSN: 1617-4623
    Keywords: Moss chloroplast DNA ; Restriction/gene map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A restriction endonuclease cleavage site map for the enzymes ClaI and BglII, and a partial map for SacI, has been constructed for the chloroplast genome of the moss Physcomitrella patens (Hedw.) BSG. The plastid chromosome contains approximately 122 kb organized into small (21 kb) and large (82 kb) single-copy regions separated by two copies of a repeat sequence (9.4 kb) oriented in an inverted arrangement. Genes for 17 proteins and 2 ribosomal RNAs have been mapped using heterologous probes from corn, spinach, pea, and petunia. The general order and arrangement of the moss chloroplast genes are similar to the consensus land plant genome typified by that of spinach, with two major exceptions. First, there is an inversion of approximately 20 kb, bordered internally by psbA and atpH, and also containing the genes atpF and atpA. Second, rpl2 and rps19 have been relocated to a different position within the large single-copy region, adjacent to the 20 kb inversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330462
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  • 4
    Electronic Resource
    Electronic Resource
    Mating systems of some putative polypore — agaric relatives (1997)
    Springer
    Plant systematics and evolution 207 (1997), S. 135-158 
    Details
    ISSN: 1615-6110
    Keywords: Agaricales ; Polyporaceae ; Mating systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mating systems are reported for taxa of polypores and agarics considered related. Taxa areDictyopanus pusillus, Favolus alveolaris, F. tenuiculus, Lentinus bertierii, L. crinitis, L. punctaticeps, L. strigellus, L. strigosus, L. suavissimus, Pleurotus australis, P. levis, P. tuberregium, Polyporus ciliatus, P. elegans, P. squamosus, andP. varius. All are tetrapolar exceptP. elegans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984386
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  • 5
    Electronic Resource
    Electronic Resource
    Relationships amongOmphalotus species (Paxillaceae) based on restriction sites in the ribosomal ITS1-5.8S-ITS2 region (1998)
    Springer
    Plant systematics and evolution 211 (1998), S. 231-237 
    Details
    ISSN: 1615-6110
    Keywords: Fungi ; Basidiomycetes ; Paxillaceae ; Omphalotus ; Restriction fragment length polymorphisms (RFLP) ; ribosomal DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic relationships for several species of the fungusOmphalotus were estimated by comparing the presence or absence of restriction sites in the ITS1-5.8S-ITS2 region of nuclear ribosomal DNA. Results placeO. olearius, O. subilludens andO. olivascens in a single clade.Omphalotus illudens, usually thought to be related toO. subilludens, was placed in a second clade, more closely related toO. nidiformis. Omphalotus mexicana is distinct from all other examinedOmphalotus species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985361
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  • 6
    Electronic Resource
    Electronic Resource
    Mating systems inOmphalotus (Paxillaceae, Agaricales) (1998)
    Springer
    Plant systematics and evolution 211 (1998), S. 217-229 
    Details
    ISSN: 1615-6110
    Keywords: Fungi ; Basidiomycetes ; Paxillaceae ; Omphalotus ; Mating systems ; speciation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pairings of monokaryon cultures representingOmphalotus illudens (eastern North America),O. nidiformis (southeastern Australia),O. olearius (southern Europe),O. olivascens (North American Pacific coast), andO. subilludens (southern North America) showed widely variable compatibility patterns.Omphalotus olearius ×O. subilludens ×O. olivascens showed high compatibility, whileO. illudens was significantly less compatible with all other taxa. Isolates ofOmphalotus nidiformis represented an almost genetically isolated biological species. The role of partial compatibility in nomenclatural ranking is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985360
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  • 7
    Electronic Resource
    Electronic Resource
    5-Azacytidine increases the activity of neomycin phosphotransferase II in transgenic Nicotiana tabacum: A posttranslational mechanism may play a role (1995)
    Springer
    Plant cell, tissue and organ culture 41 (1995), S. 49-60 
    Details
    ISSN: 1573-5044
    Keywords: 5-azacytidine ; cycloheximide ; hydroxyurea ; npt II ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In previous studies, tobacco protoplasts were transformed with the bacterial gene encoding neomycin phosphotransferase II (NPT II). Transformed calluses lost neomycin phosphotransferase II activity after several subcultures. Treatment of calluses with 5-azacytidine, a demethylating agent, restored enzyme activity, suggesting that methylation of npt II sequences might be responsible for loss of NPT II activity. Studies presented here were designed to test that hypothesis. Results indicated that the effect of 5-azacytidine could not be blocked by the DNA replication inhibitor, hydroxyurea, nor by the 5-azacytidine analogue, cytidine as would be expected with a DNA demethylation mechanism. The level of NPT II mRNA was not increased by 5-azacytidine. Treatment with cycloheximide, a protein synthesis inhibitor, had no effect on 5-azacytidine-increased NPT II activity. There was no increase of NPT II protein caused by 5-azacytidine, whereas 5-azacytidine increased activity of NPT II. In contrast, the auxin 2,4-D increased both the NPT II protein and activity. Assays for malate dehydrogenase demonstrated that the effect of 5-azacytidine and hydroxyurea on NPT II was not due to an overall effect on callus metabolism. In vitro studies involving standard bacterial NPT II enzyme and crude extracts from untreated and 5-azacytidine- or hydroxyurea-treated calluses showed that the activity of NPT II added to the untreated extracts was lower than the activity of NPT II added to the extracts from calluses treated with 5-azacytidine or hydroxyurea, indicating that there was an unknown factor (or factors) in callus extracts which affected the activity of NPT II and itself was affected by 5-azacytidine and hydroxyurea treatment. These results suggested that one effect of 5-azacytidine in increasing NPT II activity was posttranslational.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00124086
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  • 8
    Electronic Resource
    Electronic Resource
    Long term regeneration by somatic embryogenesis in barley (Hordeum vulgare L.) tissue cultures derived from apical meristem explants (1985)
    Springer
    Plant cell, tissue and organ culture 5 (1985), S. 151-162 
    Details
    ISSN: 1573-5044
    Keywords: Hordeum vulgare ; barley ; callus culture ; plant regeneration ; somatic embryogenesis ; organogenesis ; apical meristem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures were initiated from apical meristem explants of one to four-week-old aseptically-grown barley (Hordeum vulgare L. cv. Atlas 57) plants. Embryogenic callus and plants were produced in three separate experiments; the cultures have retained regenerative capacity for three years after initiation. Our results demonstrate that explants other than immature embryos are embryogenically competent in barley and that regeneration occurs by both somatic embryogenesis and organogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040312
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  • 9
    Electronic Resource
    Electronic Resource
    Ultraviolet mutagenesis of moss cells in vitro (1982)
    Springer
    Methods in cell science 7 (1982), S. 47-49 
    Details
    ISSN: 1573-0603
    Keywords: tissue culture ; moss ; ultraviolet light ; mutagenesis ; Physcomitrella patens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method using ultraviolet light as a mutagen for moss cells in tissue culture is presented. This method allows for the selection of mutants in the green plant,Physcomitrella patens (Hedw.) BSG, by varying selection techniques to fit the mutants desired and for studying the effect of ultraviolet on a green plant system. The ease by which the system can be manipulated makes it attractive for research, classroom, or demonstration needs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01665907
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of human α-interferon cDNA in transgenic rice plants (1994)
    Springer
    Plant cell, tissue and organ culture 36 (1994), S. 197-204 
    Details
    ISSN: 1573-5044
    Keywords: interferon ; Lipofectin ; protoplasts ; rice ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The plasmid pIG3031 containing human α-interferon cDNA and the neomycin phosphotransferase II coding sequence was successfully transferred into rice protoplasts (Indica type rice) by Lipofectinmediated transformation. Assays for NPT II enzyme activity indicated that the transformation frequency was 10%. Transgenic plants were regenerated from transformed calli. Southern blots showed that the human α-interferon cDNA sequence was present in rice DNA. RNA slot blots indicated apparent transcription of human α-interferon cDNA under the control of the plant-active promoter PI'. Extracts of transgenic cell cultures and plants contained apparent interferon activity as measured by resistance of a human amniotic cell line to viral infection in the presence of plant extracts. These studies demonstrate that human α-interferon cDNA may be correctly expressed in rice cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037720
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