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  • 1
    Electronic Resource
    Electronic Resource
    A novel stress-inducible metallothionein-like gene from rice (1995)
    Springer
    Plant molecular biology 28 (1995), S. 381-389 
    Details
    ISSN: 1573-5028
    Keywords: metallothionein ; Oryza sativa ; stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that rgMT encodes one protein of 74 amino acids. Differential expression of rgMT in rice plants was observed as mature transcripts were more abundant in roots than in leaves and sheaths. Under different stress conditions, such as excess heavy metals and heat shock, expression of rgMT was significantly elevated. This was especially noticeable with 250 μM CuCl2 for 16 h, 40°C heat for 2 h and 0.06% DMSO for 1 h. Under sucrose starvation, rgMT transcripts also increased with time up to 72 h. During recovery from sucrose starvation, the transcripts declined slightly within 12 h of recovery. rgMT transcripts were also seen to have increased expression in senescent leaves. These results support the notion that rgMT is a stress-inducible gene in rice heretofore unreported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020388
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    RNA expression patterns of a type 2 metallothionein-like gene from rice (1996)
    Springer
    Plant molecular biology 32 (1996), S. 525-529 
    Details
    ISSN: 1573-5028
    Keywords: metallothionein ; rice ; stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A type 2 metallothionein-like gene from rice, OsMT-2 (Oryza sativa metallothionein-like gene-2), was isolated in its cDNA form and sequenced. By northern analyses OsMT-2 expression was shown to be induced under stress by sucrose starvation, heat shock and, to a lesser extent, abscisic acid, but not excess metals, including copper. Its response to sucrose starvation was transient and different from OsMT-1, a type 1 metallothionein-like gene of rice inducible by copper. These results suggest that while OsMT-2 is also involved in cellular response to stress, its function may be complementary to that of OsMT-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019104
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    Paper (German National Licenses)
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