ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Determination of propafenone
;
5-OH propafenone
;
Reversed-phase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary This paper presents a method for the simultaneous detection of propafenone and 5-hydroxypropafenone (5-OH propafenone) using HPLC. The method is sensitive, selective, and reproducible. The chromatographic separation is based on a 25×0.4 cm 5 μm ODS column, a mobil phase of 0.1 M potassium phosphate (K2HPO4) buffer (pH 2.5) and acetonitrile (63∶37), and UV absorbance detection. There is excellent intra- and interassay variation. Retention times of the internal standard, propafenone, and 5-OH propafenone are 4.3, 6.0 and 2.9 minutes respectively. This method shows linearity over the therapeutic range for propafenone and 5-OH propafenone (0.15 to 3.0 μg/ml and 0.075 to 1.5 μg/ml respectively). No interference has been found from other commonly administered drugs, including several antiarrhythmic drugs, at therapeutic levels.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02259381
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