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  • 1
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of the regulatory nifA gene of Rhizobium leguminosarum PRE: transcriptional control sites and expression in Escherichia coli (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We report the sequence of the regulatory nifA gene of Rhizobium leguminosarum PRE. The transcription initiation and termination sites of nifA were mapped and a potential promoter and a rho-independent terminator identified. The nifA gene has two possible translation start sites, both of which are used in an Escherichia coli background, resulting in proteins with apparent molecular weights of 58kD and 57kD; initiation at the second site is preferred over initiation at the first. The nifA-nifB intergenic region contains an rpoN-dependent promoter for the nifB gene but no consensus upstream activator sequence (UAS).A potential DNA-binding domain, consisting of two α-helices separated by a four-amino-acid linker, is located at the C-terminal end of the NifA amino acid sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00127.x
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  • 2
    Electronic Resource
    Electronic Resource
    Constitutive nitrogenase synthesis from de novo transcribed mRNA in isolated Rhizobium leguminosarum bacteroids
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 740 (1983), S. 313-322 
    Details
    ISSN: 0167-4781
    Keywords: (R. leguminosarum) ; Bacteroid ; Gene expression ; Nitrogenase synthesis ; Translational regulation ; mRNA half-life
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4781(83)90140-9
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular mapping around the centromere of tomato chromosome 6 using irradiation-induced deletions (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 969-974 
    Details
    ISSN: 1432-2242
    Keywords: Key words Tomato ; Irradiation ; Mutagenesis ; Deletions ; Mapping ; Centromere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Irradiation-induced deletion mapping was exploited to construct a detailed locus-order map around the centromere of tomato chromosome 6 (CEN  6). An F1 hybrid heterozygous for the marker loci thiamineless (tl), yellow virescent (yv) and potato leaf (c), and homozygous recessive for the nematode resistance gene mi, was pollinated with γ-irradiated pollen from cultivar VFNT Cherry carrying the wild-type alleles at the corresponding loci. A dose of 100 Gy was found optimal for inducing mutants. By screening for pseudo-dominant plants showing the marker phenotypes and/or nematode susceptibility, 30 deletions encompassing one or more of the four loci were detected in the M1 generation. Molecular-marker analysis revealed that 29 of these mutants included the tl and mi loci on the short arm and originated from terminal deletions of different sizes. Remarkably, the breakpoints of these deletions were not randomly distributed along the short arm but located within the centromeric heterochromatin. Only one yv interstitial deletion and no c mutations on the long arm of the chromosome were detected. Mapping of the various chromosomal breakpoints in the isolated mutants permitted the resolution of a cluster of molecular markers from the centromeric heterochromatin that was hitherto unresolvable by genetic linkage analysis. The usefulness of such a deletion-mapping approach for whole-genome mapping is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050649
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic and physical localization of the root-knot nematode resistance locus Mi in tomato (1998)
    Springer
    Molecular genetics and genomics 257 (1998), S. 376-385 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsMeloidogyne spp. ; Resistance genes ; Lycopersicon spp. ; Mi-1 ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract As part of a map-based cloning strategy designed to isolate the root-knot nematode resistance gene Mi, tomato F2 populations were analyzed in order to identify recombination points close to this economically important gene. A total of 21 089 F2 progeny plants were screened using morphological markers. An additional 1887 F2 were screened using PCR-based flanking markers. Fine-structure mapping of recombinants with newly developed AFLP markers, and RFLP markers derived from physically mapped cosmid subclones, localized Mi to a genomic region of about 550 kb. The low frequency of recombinants indicated that recombination was generally suppressed in these crosses and that crossovers were restricted to particular regions. To circumvent this problem, a population of Lycopersicon peruvianum, the species from which Mi was originally introgressed, that was segregating for resistance was developed. Screening of this population with PCR, RFLP and AFLP markers identified several plants with crossovers near Mi. Recombination frequency was approximately eight-fold higher in the Mi region of the L. peruvianum cross. However, even within the wild species cross, recombination sites were not uniformly distributed in the region. By combining data from the L. esculentum and L. peruvianum recombinant analyses, it was possible to localize Mi to a region of the genome spanning less than 65 kb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050660
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  • 5
    Electronic Resource
    Electronic Resource
    Paracentromeric sequences on tomato chromosome 6 show homology to human satellite III and to the mammalian CENP-B binding box (1998)
    Springer
    Molecular genetics and genomics 259 (1998), S. 190-197 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsLycopersicon esculentum ; Centromere ; Deletion mapping ; CENP-B box ; Satellite III
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to isolate centromeric sequences from tomato (Lycopersicon esculentum) chromosome 6, a large-scale RAPD screen was performed. Among 1500 polymorphic RAPD markers tested, 100 were identified as chromosome 6-specific, using a L. esculentum substitution line carrying chromosome 6 from L. pennellii. Fifty-seven of these markers proved to originate from L. pennellii, and of these, 13 were genetically mapped between the morphological markers yellow virescent (yv) and thiaminless (tl), which flank the centromere. These markers could be assigned to three genetic loci, with 11 of the markers mapping to a single locus. Further resolution of this cluster was achieved using radiation-induced deletions that removed yv or tl but not the centromere. Seven markers were shown to be located outside all of the deletions. These seven markers and three of the other markers of the cluster were cloned and sequenced. Five of the clones are present as single-copy or low-copy-number sequences and five represent middle repetitive sequences. Three sequences show homology to the mammalian CENP-B binding box; clone AG12 contains two of these boxes and also shows homology to human satellite III.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050804
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