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  • 1
    Electronic Resource
    Electronic Resource
    Somatic mutations caused by excision of the transposable element, Tpn1, from the DFR gene for pigmentation in sub-epidermal layer of periclinally chimeric flowers of Japanese morning glory and their germinal transmission to their progeny (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 499-504 
    Details
    ISSN: 1432-2242
    Keywords: Transposable elements ; Periclinal chimera ; Somatic mutation ; Germinal transmission ; Japanese morning glory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pigmentation in flowers of Japanese morning glory is intense in the epidermal layer, lighter in the subepidermis, and much lighter in the internal tissues; by contrast coloration in stems occurs only in the sub-epidermal layer. The a-3 f mutant of Japanese morning glory bears white flowers with normal-colored flecks and sectors, and its variegation also occurs in leaves and stems. The mutable line can produce chimeric flowers pigmented uniformly in the sub-epidermal tissue and variegated in the epidermal layer, and stems of these flowers are also pigmented. Since they give selfed progeny that segregate to give a ratio of three germinal revertants bearing fully colored flowers to one flecked mutant, it has been [OR Imai (1934) has] postulated that somatic mutations in the sub-epidermal layer can be transmitted to the next generation and that the germ cells in the reproductive organs must form from the cells of the sub-epidermal layer. Recently, we found that the 6.4-kb En/Spm-related transposable element, Tpn1, resides within the DFR-B gene for anthocyanin biosynthesis in the mutable a-3 f line. To test whether somatic mutations caused by Tpn1 excision from the DFR-B gene in the subepidermis of periclinally chimeric flowers are transmissible to their progeny, we have examined the structure of the DFR-B region in the germinal revertants derived from the chimeric flowers and compared the sequences generated by the somatic excision of Tpn1 in periclinally chimeric flowers with those in their germinal revertants. Our results confirm that somatic mutations caused by Tpn1 excision from the DFR-B gene in the sub-epidermal tissue of chimeric flowers can be transmitted to their progeny, which results in the generation of germinal revertants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224550
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    DNA rearrangements at the region of the dihydroflavonol 4-reductase gene for flower pigmentation and incomplete dominance in morning glory carrying the mutable flaked mutation (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 509-515 
    Details
    ISSN: 1432-2242
    Keywords: Key words Allelic interaction ; Dihydroflavonol 4-reductase gene ; Mobile element-like sequences ; Morning glory ; Mutable allele
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The a-3 flecked [J] variegated line of Japanese morning glory bearing white flowers with normal-colored flecks and sectors has been shown to carry a 6.4-kb transposable element, Tpn1, inserted within the DFR-B gene, one of the anthocyanin biosynthesis genes encoding dihydroflavonol 4-reductase (DFR). The a flaked [M] variegated line of morning glory also bears white flowers with normal-colored flakes and sectors, and it was shown to carry multiple DNA rearrangements, including insertions of mobile element-like sequences, MELSIP1 and MELSIP2, in its DFR gene region. Unlike the a-3 flecked [J] mutation, the mutable a flaked [M] allele exhibited incomplete dominance. Interestingly, not only intensely colored flakes but also white spots and sectors were often observed in lightly colored flowers of morning glory in the heterozygous state A[M]/a flaked [M]. The interspecific F1 hybrids between Japanese morning glory and morning glory carrying both a-3 flecked [J]/A-3[M] and A[J]/ a flaked [M] in the heterozygous condition bear lightly colored flowers with intensely colored sectors as well as white flakes. The results clearly demonstrated that the DFR gene in the a flaked [M] line of morning glory is active and complements the DFR-B gene carrying Tpn1 in the a-3 flecked [J] line of Japanese morning glory. Interspecific allelic interactions between the mutable a flaked [M] gene of morning glory and the corresponding wild-type A[J] gene of Japanese morning glory resulted in incomplete dominance and the formation of white flakes and sectors. The appearance of the white flakes may be due to a somatic mutation of the A[J] gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050591
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  • 3
    Electronic Resource
    Electronic Resource
    The retrotransposon RTip1 is integrated into a novel type of minisatellite, MiniSip1, in the genome of the common morning glory and carries another new type of minisatellite, MiniSip2 (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1049-1056 
    Details
    ISSN: 1432-2242
    Keywords: Key words DNA rearrangement ; Minisatellite ; Morning glory ; Repetitive DNA ; Retrotransposon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transposable elements have often been discovered as new insertion sequences in known genes, and minisatellites are often employed as molecular markers in diagnostic and mapping studies. We compared the genes for flower pigmentation in a line of the common morning glory bearing fully colored flowers with those in two anthocyanin flaked mutable lines producing variegated flowers and found RFLPs at the region of the ANS gene for anthocyanin biosynthesis. The DNA rearrangements detected by the RFLPs are due to integration of a novel type of minisatellite, MiniSip1, having a long LTR retrotransposon, RTip1, inserted in the mutable lines. The structural analysis of the rearranged region revealed that the 12.4-kb RTip1 element is flanked by 5-bp target duplications within the MiniSip1 sequence and contains two LTR sequences of about 590 bp, a primer binding site for tRNALys, a typical polypurine tract and another new type of minisatellite, MiniSip2. Since no long open reading frame corresponding to the gag and pol genes was found, RTip1 appears to be a defective Ty3/gypsy-like element. Interestingly, the 269-bp-long MiniSip1 element comprises two alternating motifs of 41 bp and 19 bp, whereas the 962 bp long MiniSip2 element consists of two partially alternating motifs of 86 bp and 90 bp which are partially homologous to each other. Possible evolutionary processes that may have generated the rearranged structure at the ANS gene region are also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050661
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  • 4
    Electronic Resource
    Electronic Resource
    Somatic mutations caused by excision of the transposable element, Tpn1, from the DFR gene for pigmentation in sub-epidermal layer of periclinally chimeric flowers of Japanese morning glory and their germinal transmission to their progeny (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 499-504 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Transposable elements ; Periclinal chimera ; Somatic mutation ; Germinal transmission ; Japanese morning glory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Pigmentation in flowers of Japanese morning glory is intense in the epidermal layer, lighter in the sub-epidermis, and much lighter in the internal tissues; by contrast coloration in stems occurs only in the sub-epidermal layer. The a-3 f〉 mutant of Japanese morning glory bears white flowers with normal-colored flecks and sectors, and its variegation also occurs in leaves and stems. The mutable line can produce chimeric flowers pigmented uniformly in the sub-epidermal tissue and variegated in the epidermal layer, and stems of these flowers are also pigmented. Since they give selfed progeny that segregate to give a ratio of three germinal revertants bearing fully colored flowers to one flecked mutant, it has been [OR Imai (1934) has] postulated that somatic mutations in the sub-epidermal layer can be transmitted to the next generation and that the germ cells in the reproductive organs must form from the cells of the sub-epidermal layer. Recently, we found that the 6.4-kb En/Spm-related transposable element, Tpn1, resides within the DFR-B gene for anthocyanin biosynthesis in the mutable a-3 f line. To test whether somatic mutations caused by Tpn1 excision from the DFR-B gene in the sub-epidermis of periclinally chimeric flowers are transmissible to their progeny, we have examined the structure of the DFR-B region in the germinal revertants derived from the chimeric flowers and compared the sequences generated by the somatic excision of Tpn1 in periclinally chimeric flowers with those in their germinal revertants. Our results confirm that somatic mutations caused by Tpn1 excision from the DFR-B gene in the sub-epidermal tissue of chimeric flowers can be transmitted to their progeny, which results in the generation of germinal revertants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050155
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  • 5
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    Efficient transfer of base changes from a vector to the rice genome by homologous recombination: involvement of heteroduplex formation and mismatch correction (2008)
    Oxford University Press
    Details
    Publication Date: 2008-06-27
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 6
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    Rayleigh-Taylor instability growth control by an oscillating acceleration in heavy ion inertial fusion (2010)
    Institute of Physics
    Details
    Publication Date: 2010-08-01
    Print ISSN: 1742-6588
    Electronic ISSN: 1742-6596
    Topics: Physics
    Published by Institute of Physics
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