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  • 1
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The objective of this study was to evaluate the secretory IgA (SIgA) antibody response to Naegleria fowleri (Nf) in individuals living in a parasite endemic area. Saliva and serum samples were obtained from both healthy subjects and patients suffering from a respiratory illness (chronic bronchitis or rhinitis) and were analyzed by immunoblot assay. SIgA from the patients' samples recognized more intensely a greater number of Nf proteins than did SIgA from the healthy control group. The proteins more frequently recognized were those with a molecular weight of 171, 107, 102, 62, 50, 46, and 10 kDa. Some IgA antibodies recognized proteins from Nf and Entamoeba histolytica (Eh) of similar molecular weight. These results suggest that some of those antibodies could have been elicited by a previous intestinal infection with Eh. Through the common mucosal immune system the IgA B-cells activated by Eh antigens can be disseminated to all the mucosae, including the nasal mucosa. SIgA antibodies recognizing Nf proteins, induced either by specific immunization or by cross-reaction, could participate in the resistance to the infection, probably by inhibiting the adherence of Nf trophozoites to the nasal mucosa.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2015-11-01
    Print ISSN: 0379-6779
    Electronic ISSN: 1879-3290
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Elsevier
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  • 3
    Publication Date: 2019-07-01
    Print ISSN: 0014-3057
    Electronic ISSN: 1873-1945
    Topics: Chemistry and Pharmacology , Physics
    Published by Elsevier
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  • 4
    Publication Date: 2021-05-19
    Description: En el presente trabajo se realizó un estudio de las relaciones evolutivas de las especies representativas de peces del género Lucifuga en Cuba mediante el análisis de sus variaciones genéticas y moleculares. Se estudiaron 11 sistemas proteicos y la secuencia parcial del gen mitocondrial citocromo b (cytb). Los animales fueron colectados en diferentes localidades de la zona occidental de Cuba. La filogenia reconstruida a partir de los caracteres moleculares indica la existencia de dos linajes evolutivos fundamentales cuya divergencia está sustentada por 11 loci proteicos, 73 sinapomorfías mitocondriales y un uso diferencial de los codones que codifican para el aminoácido valina. Uno de los linajes está constituido por las especies L. dentatus, L. dentatus var. holguinensis y L. simile y el otro por las especies L. subterraneus y L. teresinarum. La distribución de las especies en los linajes evolutivos se corresponde con la asignación de las mismas a los dos subgéneros definidos para este género. El análisis filogenético sostiene que las especies L. simile, L. dentatus y L. subterraneus constituyen linajes evolutivos independientes caracterizados por múltiples sinapomorfías, coincidiendo con clasificación taxonómica actual. L. teresinarum no se sustenta como clado monofilético y apunta a ser una sinonimia de L. subterraneus, aunque los resultados al respecto no son concluyentes. Los ejemplares de la denominada L. dentatus var. holguinensis capturados en dos localidades del norte de Matanzas constituyen un clado bien soportado definido por caracteres moleculares, bioquímicos y morfológicos. Esto sugiere la revisión de su estatus taxonómico.
    Description: Unpublished
    Description: Lucifuga (Ophidiiformes: Bythitidae)
    Description: Cuba
    Keywords: Genetics ; Evolution (organisms) ; Molecular taxonomy
    Repository Name: AquaDocs
    Type: Theses and Dissertations , Master thesis
    Format: 81
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  • 5
    Publication Date: 2021-05-19
    Description: FourmicrosatellitelociwereusedtoachievegeneticcharacterizationofsixstocksfromLitopenaeusvannamei usedforaquaculture inCuba:secondgenerationfromfirstintroduction(S2-1),firstgenerationfromthesecondone(S1-2),fromthethirdone(S1-3), andthefourthone(S1-4)andthecrossingsfromtwoparentalpopulation:firstgenerationfromthefirstwithfirstgeneration fromthethird(S1-1 × S1-3)andfirstgenerationfromthesecondwithfirstgenerationfromthethird(S1-2 × S1-3).66%(16/24) ofgeneticsystemsintotallociwereingeneticdisequilibrium.Thefourmicrosatellitelociwerepolymorphicforallsixstocks. MajorquantitiesofallelicvariantscorrespondtolocusPvan1758,whichisatthesametimethatonewherethereareprivate allelesfromfirstgenerationofthethird.AllFst comparisonsweresignificant.Thisindicatesbigdifferencesbetweenstocks.The highestvaluesarethoseinwhichthereispresenceofthesecondintroduction.Thisintroductionanditsdescendantsarealsomore consanguineous.
    Description: Published
    Description: Litopenaeus vannamei
    Description: Cuba
    Keywords: Genetics ; Aquaculture ; Shrimp culture
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 6
    Publication Date: 2021-05-19
    Description: El objetivo del presente trabajo fue obtener información preliminar acerca de la composición de haplotipos del ADN mitocóndrico (ADNmt) del manatí (Trichechus manatus) que habita en el archipiélago cubano. Se analizó un total de 13 individuos, 12 de Cuba y uno procedente de la Florida, EU. Las secuencias de un fragmento de 410 pb de la región de control del ADNmt (D-loop) permitieron identificar dos haplotipos. El haplotipo A1, único encontrado en la Florida (incluida la muestra aquí analizada), pero también presente en México, Puerto Rico y República Dominicana. Este haplotipo fue mayoritario (11 de los 12 individuos muestreados en Cuba). El segundo haplotipo fue el A3, referido anteriormente como endémico de Belice, se encontró en un individuo varado en Isabela de Sagua, al norte de Cuba. Los resultados preliminares, sobre la base de las muestras examinadas y el marcador analizado, brindan información importante sobre tres aspectos fundamentales de la biología del manatí: 1) En Cuba la diversidad genética del ADNmt puede ser baja, similar a lo encontrado en otras partes de su ámbito de distribución; 2) los individuos analizados forman parte del grupo constituido por la Florida y las Antillas Mayores; 3) los territorios de Belice y Cuba han intercambiado individuos en el presente o en un pasado relativamente reciente.
    Description: The aim of this analysis was to obtain information regarding the mtDNA haplotype composition of the manatee (T. manatus) occupying the Cuban archipelago. A fragment of 410 bp of the non coding region was analyzed for 12 individual manatees from Cuba and one from Florida, USA. Only two haplotypes were identified. Haplotype A1, found exclusively in Florida (including in the sample analyzed here) but also found in Mexico, the Dominican Republic and Puerto Rico, was the most frequent haplotype (11 of the 12 samples from Cuba) and widely distributed. The second haplotype A3, previously referred to as endemic from Belize, was identified from an individual stranded in Isabela de Sagua, north of Cuba. These preliminary results provide information about three major aspects of manatee biology: (1) the mtDNA genetic diversity of T. manatus in Cuba seems low as compared to other regions of the Caribbean; (2) the Cuban population likely belongs to the group comprising Florida and the portions of the Greater Antilles; and (3) the territories of Belice and Cuba have exchanged individuals at present or in a relatively recent past.
    Description: Published
    Description: haplotype
    Description: Caribbean
    Description: Trichechidae
    Keywords: Genetic diversity ; mtDNA
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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