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  • 1
    Electronic Resource
    Electronic Resource
    A Comparison of Different Approaches To Calculate Surface Free Energies of Protein-Coated Substrata from Measured Contact Angles of Liquids (1994)
    s.l. : American Chemical Society
    Langmuir 10 (1994), S. 1314-1318 
    Details
    ISSN: 1520-5827
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/la00016a055
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  • 2
    Electronic Resource
    Electronic Resource
    Physico-chemical surface characteristics and adhesive properties of Streptococcus salivarius strains with defined cell surface structures (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 40 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Physico-chemical surface characteristics and adhesive properties of a series of mutants of Streptococcus salivarius HB with defined cell surface structures were determined. Zeta potentials showed no relation either with the presence or absence of specific antigens on the bacterial cell surface, or with the adhesive properties of the cells. Hydrophobicity was assessed by surface free energy determination from measured contact angles, by adsorption to hexadecane and by hydrophobic interaction chromatography. Generally, the progressive removal of fibril subclasses from the cell surface resulted in a reduced hydrophobicity. However, specific fibrillar subclasses appeared to contribute to surface hydrophobicity to widely different extents. Bacterial adhesion to polymethylmethacrylate increased with increasing hydrophobicity of the mutants. However, adhesion to a more complex biological substratum, such as saliva-coated hydroxyapatite, correlated only partly with hydrophobicity. The organism, deprived of most of its fibrillar surface structures, clearly showed the least adhesion to hydrophobic ligands, to both polymethylmethacrylate and saliva-coated hydroxyapatite, and had a significantly higher surface free energy than the other mutants and the parent strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb01974.x
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of aeration of Candida albicans during culturing on their surface aggregation in the presence of adhering Streptococcus gordonii (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 26 (1999), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Candida albicans surfaces are extremely sensitive to changes in growth conditions. In this study, adhesion to glass of aerated and non-aerated C. albicans ATCC 10261 in the presence and absence of adhering Streptococcus gordonii NCTC 7869 was determined in a parallel plate flow chamber. In addition, the influence of aeration on the yeast cell surface hydrophobicity, surface charge, and elemental cell surface composition was measured. S. gordonii adhering at the glass surface caused a reduction in the initial deposition rate of C. albicans, regardless of aeration. In a stationary end-point, only adhesion of non-aerated C. albicans was suppressed by the adhering S. gordonii. Non-aerated yeasts had a higher O/C elemental surface concentration ratio, indicative of cell surface polysaccharides, than aerated yeasts, at the expense of nitrogen-rich cell surface proteins. Both yeasts were essentially uncharged, but the nitrogen-rich cell surface of aerated yeasts had a slightly higher water contact angle than non-aerated yeasts. Summarizing, this study suggests that highly localized, hydrophobic cell surface proteins on C. albicans are a prerequisite for their interaction with adhering streptococci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1999.tb01373.x
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  • 4
    Electronic Resource
    Electronic Resource
    Purification and characterization of a surface-binding protein from Lactobacillus fermentum RC-14 that inhibits adhesion of Enterococcus faecalis 1131 (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 190 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. One strain, Lactobacillus fermentum RC-14, releases surface-active components which can inhibit adhesion of uropathogenic bacteria. Using a quantitative method for determining inhibition of adhesion, a protein with high anti-adhesive properties against Enterococcus faecalis 1131 was purified. The N-terminal sequence of the 29-kDa protein was identical to that of a collagen-binding protein from Lactobacillus reuteri NCIB 11951, and exhibited close homology with a basic surface protein from L. fermentum BR11. The results suggest that this anti-adhesive cell surface protein of Lactobacillus could protect against uropathogens by preventing their adhesion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09282.x
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  • 5
    Electronic Resource
    Electronic Resource
    Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for study (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 23 (1999), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this review, initial microbial adhesive interactions are divided into adhesion to substratum surfaces, coaggregation between microbial pairs and co-adhesion between sessile and planktonic microorganisms of different strains or species. The physico-chemical mechanisms underlying the adhesive interactions are described and a critical review is given of currently employed methods to study microbial adhesive interactions, with an emphasis on the use of the parallel plate flow chamber. Subsequently, for each of the three microbial adhesive interactions distinguished, the role of Lifshitz-van der Waals, acid-base and electrostatic interactions is described based on existing literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1999.tb00396.x
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  • 6
    Electronic Resource
    Electronic Resource
    Adhesive interactions between medically important yeasts and bacteria (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 21 (1998), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Yeasts are being increasingly identified as important organisms in human infections. Adhesive interactions between yeasts and bacteria may contribute to yeast retention at body sites. Methods for studying adhesive interactions between bacterial strains are well known, and range from simple macroscopic methods to flow chamber systems with complex image analysis capabilities. The adhesive interactions between bacteria and yeasts have been studied employing several of the methods originally developed for studying adhesive interactions between bacteria. However, in many of the methods employed the larger size of the yeasts as compared with bacteria results in strong sedimentation of the yeasts, often invalidating the method adapted. In addition, most methods are semi-quantitative and do not properly control mass transport. Consequently, adhesive interaction mechanisms between yeasts and bacteria identified hitherto, including lectin binding and protein-protein interactions, must be regarded with caution. Extensive physico-chemical characteristics of yeast cell surfaces are not available and a physico-chemical mechanism has not yet been put forth. A new method for quantifying adhesive interactions between yeasts and bacteria is proposed, based on the use of a parallel plate flow chamber, in which the influence of adhering bacteria upon the kinetics of yeast adhesion and aggregation of the adhering yeasts is quantitatively evaluated, under carefully controlled mass transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1998.tb00356.x
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  • 7
    Electronic Resource
    Electronic Resource
    Adhesion of Enterococcus faecalis 1131 grown under subinhibitory concentrations of ampicillin and vancomycin to a hydrophilic and a hydrophobic substratum (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 203 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of two subinhibitory antibiotic concentrations of ampicillin and vancomycin during growth on the adhesion of Enterococcus faecalis 1131 to glass and silicone rubber was studied in a parallel plate flow chamber. Initial deposition rates and numbers of adhering bacteria after 4 h were higher on hydrophilic glass than on hydrophobic silicone rubber, regardless of growth conditions. The presence of 1/4 minimal inhibitory concentration (MIC) of ampicillin during growth reduced enterococcal adhesion to both substrata, but growth in the presence of 1/4 MIC vancomycin did not affect the adhesion of E. faecalis. Moreover, enterococcal adhesion increased after growth in the presence of 1/8 MIC vancomycin. The increased adhesion after growth in the presence of subinhibitory concentrations of vancomycin may have strong implications for patients living with implanted biomaterials, as they may suffer adverse effects from use of this antibiotic, especially since bacteria once adhered are less sensitive to antibiotics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10823.x
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  • 8
    Electronic Resource
    Electronic Resource
    Uropathogenic Escherichia coli adhere to urinary catheters without using fimbriae (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 16 (1996), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract Five well-characterized urinary and fecal isolates of Escherichia coli were found to be hydrophilic irrespective of their serotypes and their ability to express fimbriae. All the strains were able to adhere to silicone latex urinary catheters, although strain 917, which expressed type P fimbriae as its only adhesin, adhered poorly. Although specific adhesins, particularly fimbriae, have been shown to mediate adhesion of E. coli to uroepithelial cells, they do not mediate specific adhesion onto urinary catheter material. The overall surfaces of the strains, tested using microelectrophoresis as a function of pH and X-ray photoelectron spectroscopy, were not significantly different, thus suggesting more non-specific adhesion mechanisms to urinary catheters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1996.tb00132.x
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  • 9
    Electronic Resource
    Electronic Resource
    Correlation between hydrophobicity and resistance to nonoxynol-9 and vancomycin for urogenital isolates of lactobacilli (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 94 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Seven clinical isolates of lactobacilli were found to be relatively hydrophobic with a mean watercontact angle of 66 ± 15 degrees, and to be susceptible to 1% nonoxynol-9 and vancomycin. However, seven other strains were relatively hydrophilic with a mean water-contact angle of 32 ± 13 degrees, and found to be resistant to 25% nonoxynol-9 and vancomycin. Thus, the surface properties of lactobacilli that influence susceptibility to antimicrobial agents may involve surface hydrophobicity. Possibly the penetration barrier posed by the cell surface towards these two non-ionic antimicrobials is lower for hydrophobic cells than for hydrophilic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05297.x
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  • 10
    Electronic Resource
    Electronic Resource
    Surface free energies of oral streptococci (1984)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 25 (1984), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The surface free energies (γb) of a variety of oral streptococci were determined from contact angle measurements on bacterial deposits, using the concept of dispersion and polar components. At least four strains of each species were tested. Strains of Streptococcus mutans, S. sanguis and S. salivarius possessed relatively high surface free energies (103 ± 12 mJ · m−2) and at the species level no significant difference was found. In contrast, the strains of S. mitis had remarkably low surface free energies (45 ± 14 mJ · m−2). S. milleri appeared to be a heterogeneous species, showing surface free energies over a range of 32–119 mJ · m−2. No significant differences were observed between laboratory strains and strains freshly isolated from the oral cavity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1984.tb01472.x
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