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  • 1
    Electronic Resource
    Electronic Resource
    Genetic aspects of the organization of legumin genes in pea (1993)
    Springer
    Plant molecular biology 22 (1993), S. 101-112 
    Details
    ISSN: 1573-5028
    Keywords: pea ; Pisum sativum ; recombination ; physical & genetic maps ; legumin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to be determined. The relationship between physical and genetic distances in this region is discussed, as are the implications of this for relating physical and genetic maps elsewhere in the pea genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00038999
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis (1990)
    Springer
    Plant molecular biology 15 (1990), S. 707-722 
    Details
    ISSN: 1573-5028
    Keywords: retrotransposon ; Pisum ; segregation ; PCR ; RFLP ; mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA sequence between two legumin genes in Pisum is a member of the copia-like class of retrotransposons and represents one member of a polymorphic and heterogeneous dispersed repeated sequence family in Pisum. This sequence can be exploited in genetic studies either by RFLP analysis where several markers can be scored together, or the segregation of individual elements can be followed after PCR amplification of specific members.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016121
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  • 3
    Electronic Resource
    Electronic Resource
    Repeated sequences as genetic markers in pooled tissue samples (1993)
    Springer
    Plant molecular biology 22 (1993), S. 153-157 
    Details
    ISSN: 1573-5028
    Keywords: linkage ; DNA mixtures ; repeated sequences ; pea ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We show, using the PDR1 element of pea, that dispersed repeated sequences of moderate copy number can be used simply and efficiently to generate markers linked to a trait of interest. Inspection of hybridization patterns of repeated sequences to DNA mixtures of pooled genotypes is a sensitive way of detecting such markers. The large number of bands in tracks of digests of these mixtures allows the simultaneous sampling of loci at many places in the genome, and the many unlinked loci serve as internal controls. It is also shown that intensity ratios calculated from these band differences can be used to give a rough estimate of linkage distance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039003
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    Paper (German National Licenses)
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