ISSN:
1573-6822
Keywords:
general toxicity
;
testing
;
validation
;
in vitro cytotoxicity
;
toxicokinetics
;
multivariate toxicity modeling
;
basal cytotoxicity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A new international project to evaluate the relevance for human systemic and local toxicity of in vitro tests of general toxicity of chemicals has been organized by the Scandinavian Society of Cell Toxicology under the title 1Multicenter Evaluation of In Vitro Cytotoxicity (MEIC). The basic assumptions underlying the project, as well as the practical goals and the design of the program are outlined. The list of the first 50 reference chemicals is presented. The chemicals are an otherwise unbiased selection of compounds with known human acutely lethal dosage and blood concentrations, including LD50-values in the rat or mouse. Most agents also have other data on human toxicity and toxicokinetics, including more extensive animal toxicity data. International laboratories already using or developing in vitro tests of various partial aspects of general toxicity are invited to test the substances, the results of which will be evaluated by us. The predictivity of the in vitro results for both partial and gross human toxicity data will be determined with combined use of univariate regression analysis and soft multivariate modeling. The predictivity of the in vitro results will be compared with the predictivity of conventional animal tests for the same chemicals. Finally, batteries of tests with optimal prediction power for various types of human toxicity will be selected. The need for and possible uses of such batteries are discussed. TABLE 1 The First 50 Reference Chemicals of the MEIC Project 1. Acetaminophen 26. Arsenic trioxide 2. Aspirin 27. Cupric sulfate 3. Ferrous sulfate 28. Mercuric chloride 4. Diazepam 29. Thioridazine HCl 5. Amitriptyline 30. Thallium sulfate 6. Digoxin 31. Warfarin 7. Ethylene glycol 32. Lindane 8. Methyl alcohol 33. Chloroform 9. Ethyl alcohol 34. Carbon tetrachloride 10. Isopropyl alcohol 35. Isoniazid 11. 1,1,1-Trichloroethane 36. Dichloromethane 12. Phenol 37. Barium nitrate 13. Sodium chloride 38. Hexachlorophene 14. Sodium fluoride 39. Pentachlorophenol 15. Malathion 40. Verapamil HCl 16. 2,4-Dichlorophenoxyacetic acid 41. Chloroquine pholphate 17. Xylene 42. Orphenadrine HCl 18. Nicotine 43. Quinidine sulfate 19. Potassium cyanide 44. Diphenylhydantoin 20. Lithium sulfate 45. Chloramphenicol 21. Theophylline 46. Sodium oxalate 22. Dextropropoxyphene HCl 47. Amphetamine sulfate 23. Propranolol HCl 48. Caffeine 24. Phenobarbital 49. Atropine sulfate 25. Paraquat 50. Potassium chloride
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01795360
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