Publication Date:
2017-07-07
Description:
The interactions between the trinuclear cyanine all- trans -2,2'-[3-[(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)ethylidene]-1,4-pentadiene-1,5-diyl]-bis[1,3,3-trimethyl]-3H-indolium with representative G4-DNA sequences, namely, 22AG [d(AG 3 T 2 AG 3 T 2 AG 3 T 2 AG 3 )], c-myc [d(TGAG 3 TG 3 TAG 3 TG 3 TA 2 )], c-kit [d(AG 3 AG 3 CGCTG 3 AG 2 AG 3 )], and a2 [d(ACAG 4 TGTG 4 ACAG 4 TGTG 4 )] and with double-stranded DNA, ct DNA and ds26 [d(CA 2 TCG 2 ATCGA 2 T 2 CGATC 2 GAT 2 G)], are studied by photometric and fluorimetric titrations, CD-spectroscopic analysis, and 1 H-NMR spectroscopy. It is demonstrated that the heptamethine cyanine binds to quadruplex DNA by terminal π-stacking and to duplex DNA by aggregation along the DNA backbone. The ligand does not bind selectively to quadruplex DNA as compared with duplex DNA, but the two DNA forms can be differentiated with this ligand based on the different development of its absorption and emission properties upon ligand-DNA association. The trinuclear [2.2.2.]heptamethine cyanine enables the discrimination between quadruplex and duplex DNA based on the different developments of absorption and emission properties upon ligand-DNA association.
Print ISSN:
0894-3230
Electronic ISSN:
1099-1395
Topics:
Chemistry and Pharmacology
,
Physics
Permalink
