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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 87 (1993), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cellular responses of two rapid-cycling Brassica species. B. napus and B. carinata, to seawater salinity were characterized to determine whether callus showed a tolerance similar to that of whole plants. Callus was initiated from the leaves of 7-day-old seedlings of B. napus and B. carinata and then subcultured with two different levels of seawater salinity (2.3 and 5.2 g l−1 Instant Ocean. Aquarium Systems, Inc. Mentor. OH, USA) for 14 days. Callus growth of both species was reduced by seawater salinity. Based on the percentage of the reduction in the relative fresh weight gain. B. napus was more salt-tolerant than B. carinata. consistent with the response of whole plants of the same species to seawater salinity. Seawater salinity caused changes in the concentrations of Na, K. Ca, Mg and Cl in both B. napus and B. carinata. The growth expressed as the percentage of control was significantly (P = 0.05) positively correlated with the concentration of Ca. and K/Na and Ca/Na ratios. It was also negatively correlated (P= 0.01) with the concentrations of Na. Cl and Mg. In comparison with B. carinala. the salt-tolerant species, B. napus, showed a small reduction in the concentrations of Ca and K in the salt-stressed plants relative to the control.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 139 (1992), S. 285-294 
    ISSN: 1573-5036
    Keywords: Brassica ; B. campestris ; B. carinata ; B. juncea ; B. naptus ; B. nigra ; B. oleracea ; calcium ; chloride ; potassium ; magnesium ; nitrogen ; sodium ; nutrients ; salinity ; salt-tolerance ; seawater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The growth of six rapid-cycling lines of Brassica species, B. napus, B. campestris, B. nigra, B. juncea, B. oleracea and B. carinata was inhibited by seawater salinity. Based on the change in dry matter reduction relative to the control at varying concentrations of salts (4, 8 and 12 dS m-1), the relative salt tolerance of these species was evaluated. B. napus and B. carinata were the most tolerant and most sensitive species, respectively, while the other four species were moderately tolerant. The influence of seawater on the concentrations of 12 elements including macronutrients and micronutrients in the shoots of these Brassica plants was characterized to determine the relationship between nutritional disturbance and relative salt tolerance. It was found that seawater salinity had a significant effect on the concentrations of Ca, Mg, K, Cl, Na and total N in the shoots of these plants but only the change in Ca concentration was significantly related to the relative salt tolerance of these six rapid-cycling Brassica species according to a rank analysis of the data. This finding indicates that Ca may play a regulatory role in the responses of Brassica species to saline conditions.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 153 (1993), S. 19-31 
    ISSN: 1573-5036
    Keywords: Brassica carinata ; Brassica napus ; calcium ; chloride ; growth analysis ; leaf area ratio ; magnesium ; net assimilation rate ; potassium ; relative growth rate ; seawater ; sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The response of two rapid-cycling Brassica species differing in tolerance to seawater salinity was studied over a period of 24 days. In response to 8 dS m−1 salinity, the two Brassica species showed clear differences in the changes in relative growth rate (RGR), net assimilation rate (NAR) and leaf area ratio (LAR). The RGR of B. napus was slightly reduced by salinity, wheareas the RGR of B. carinata was largely reduced in the early stages of salinization. LAR of B. napus was affected by salinity in the later stages of growth and significantly correlated with the reduction in RGR. On the other hand, the NAR of B. carinata was decreased by salinity, corresponding to the decrease of the RGR of B. carinata. The NAR of B. napus was not significantly affected by salinity according to analysis of covariance. The shoot concentrations of Na, Mg and Cl increased while the concentrations of K and Ca decreased sharply during the first 5 days of salinization; subsequently, all ion concentrations remained relatively constant. The concentrations of Na, K, Ca, Mg and Cl in the root were similarly affected by salinity. There were no significant differences of ion concentrations between species that could be related to the differences in salt tolerance. Thus, the differences in salt tolerance between species can not be related to differences in specific-ion effects, but may be related to some factor that reduces the NAR of B. carinata during the early stages of growth.
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  • 4
    ISSN: 1573-5036
    Keywords: abscisic acid ; Brassica napus ; Brassica carinata ; callus ; photosynthesis ; plant growth ; salinity ; salt tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The greater sensitivity of B. carinata to salinity in comparison to B. napus has been linked to a greater reduction in net assimilation rate. Apparently this is not due to ion toxicity; the cause is unknown. In this report, we test the hypothesis that increases in abscisic acid (ABA) are involved in the reduction of growth by salinity. Salinity (8 dS m−1) caused an increase of ABA concentrations in the shoot, root and callus of both species. ABA concentrations were lower in the salt-tolerant species, B. napus, than the salt-sensitive species, B. carinata, both in the whole plant and callus. Leaf expansion for both species was equally sensitive to ABA; salt stress did not significantly alter sensitivity to applied ABA. The growth inhibition increased in a hyperbolic manner with an increase in endogenous ABA concentration. These results indicate that ABA in salt-stressed plants may play a role in the inhibition of growth. The photosynthesis of salt-sensitive species, B. carinata, was also decreased by salinity, corresponding to the reduction in growth. The decreased photosynthesis does not appear to be the cause of the growth reduction, because photosynthesis was not inhibited by short-term exposure to salinity and photosynthesis was poorly correlated with endogenous ABA concentrations.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 155-156 (1993), S. 415-418 
    ISSN: 1573-5036
    Keywords: Brassica ; calcium ; growth ; salinity ; salt tolerance ; seawater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Whole plant and callus cultures of different rapid-cycling Brassica species were treated with salinity (8 dS m-1) and/or supplemental Ca (up to 10 mM total concentration). None of these cultures responded to supplemental Ca with improved growth indicating that the salt tolerance of these genotypes was not dependent upon Ca.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2014-12-24
    Description: ABSTRACT Previous studies have shown that biological effect of particulate matter (PM2.5) is involved in including chemical composition and mass concentration, but the precise components and biological action on human bronchial epithelial cell line (BEAS-2B) are still unclear. The aim of this study was to evaluate the in vitro toxicity of PM2.5 collected at six urban sites in China, and to investigate how particle composition affects cytotoxicity. We used human bronchial epithelial (BEAS-2B) cell lines as model in vitro to expose to PM2.5 from different source, and then reactive oxygen species (ROS), superoxide dismutase activity and total antioxidant capacity were analyzed. Furthermore, we estimated the polycyclic aromatic hydrocarbon (PAH) and transition metal and the endotoxin contents. The mRNA expression of IL-1β and IL-10 following exposure to PM2.5 was measured by QRT-PCR. We also observed the mitochondrial membrane potential (MMP) using JC-1 staining, and apoptosis of BEAS-2B using flow cytometry. In addition, double-stranded DNA breaks (DSBs) were assessed using γ-H2AX immunofluorescence. Our results show that high concentrations of PAHs and elemental Ni were strongly associated with high apoptosis rates and high expression of IL-1β, in addition, Fe element was associated with the ROS level, furthermore, Fe and Cr element were associated with DNA damage in BEAS-2B cells. The cytotoxic effects of urban PM2.5 derived from six different cities in China appear dependent on the specific components in each. Our results indicate that air quality standards based on PM2.5 components may be more relevant than concentration–response functions (CRF). © 2014 Wiley Periodicals, Inc. Environ Toxicol, 2014.
    Print ISSN: 1520-4081
    Electronic ISSN: 1522-7278
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Wiley
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  • 7
    Publication Date: 2017-07-08
    Description: MiR-497 ∼ 195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1α activity Nature Communications, Published online: 7 July 2017; doi:10.1038/ncomms16003 H-type endothelium, defined by the high expression of CD31 and endomucin, is found in the bone where it promotes angiogenesis and osteogensis. Here Yang et al . show that the miR-497 ∼ 195 cluster regulates the generation and maintenance of the H-type endothelium by controlling the levels of Notch regulator Fbxw7 and the HIF regulator P4HTM.
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
    Published by Springer Nature
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  • 8
    Publication Date: 1996-02-01
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 9
    Publication Date: 1992-01-01
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 10
    Publication Date: 1993-10-01
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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