ISSN:
1432-0983
Keywords:
2 micron DNA
;
Curing
;
S. cerevisiae
;
Yeast vectors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with these plasmids offers a general method of obtaining ciro derivatives of any yeast strain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00355406
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