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  • 1
    Publication Date: 2015-02-05
    Description: Plasma medicine is an upcoming research area that has attracted the scientists to explore more deeply the utility of plasma. So, apart from the treating biomaterials and tissues with plasma, we have studied the effect of soft plasma with different feeding gases such as Air, N2 and Ar on modification of biomolecules. Hence, in this work we have used the soft plasma on biomolecules such as proteins ((Hemoglobin (Hb) and Myoglobin (Mb)), calf thymus DNA and amino acids. The structural changes or structural modification of proteins and DNA have been studied using circular dichroism (CD), fluorescence spectroscopy, protein oxidation test, gel electrophoresis, UV-vis spectroscopy, dynamic light scattering (DLS) and 1D NMR, while Liquid Chromatograph/Capillary Electrophoresis-Mass Spectrometer (LC/CE-MS) based on qualitative and quantitative bio-analysis have been used to study the modification of amino acids. Further, the thermal analysis of the protein has been studied with differential scanning calorimetry (DSC) and CD. Additionally, we have performed docking studies of H2O2 with Hb and Mb, which reveals that H2O2 molecules preferably attack the amino acids near heme group. We have also shown that N2 gas plasma has strong deformation action on biomolecules and compared to other gases plasma. Scientific Reports 5 doi: 10.1038/srep08221
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Springer Nature
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  • 2
    Publication Date: 2015-09-16
    Description: Non-thermal plasma is used here for membrane oxidation and permeabilization in which the electrical properties of an erythrocyte membrane are investigated after treatments. The zeta potential as measured by electrophoresis shows the increased negativity of the membrane surface potential (Ψ s ). The secondary electron emission coefficient ( γ ) measured by a focused ion beam shows a decrease in the dipole potential (Ψ d ) of lipid molecules. The voltage-sensitive fluorescent intensity as measured by flow cytometry shows a decrease in the trans-membrane potential (ΔΨ) through the lipid bilayer membrane. These results allow us to take a step forward to unveil the complex events occurring in plasma-treated cells.
    Print ISSN: 0003-6951
    Electronic ISSN: 1077-3118
    Topics: Physics
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  • 3
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    American Institute of Physics (AIP)
    Publication Date: 2015-12-10
    Description: Research on the generation of radicals in nitrogen plasma shows that the most dominant radicals are excited nitrogen molecules in the metastable state of N 2 (A 3 ∑ u + ). Hydroxyl molecules are generated from the dissociation of water molecules upon contact with excited nitrogen molecules. The estimated densities of various radicals in nitrogen plasma with an electron temperature of 1 eV are presented in this study. The behavior of these radicals in media is also investigated. Excited nitrogen molecules in the N 2 (A 3 ∑ u + ) state from a plasma jet are injected into water, after which the molecules disappear instantaneously within a few tens of nm, producing hydroxyl molecules. Hydrogen peroxide, hydrogen dioxide, and nitrogen monoxide molecules can diffuse much deeper into water, implying the possibility that a chemical reaction between hydrogen dioxide and nitrogen monoxide molecules produces hydroxyl molecules in deep water, even though density in this case may not be very high.
    Print ISSN: 1070-664X
    Electronic ISSN: 1089-7674
    Topics: Physics
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  • 4
    Publication Date: 2014-08-21
    Description: Heavy water (D 2 O) was introduced into a non-thermal plasma-jet (NTPJ) device to generate deuterium monoxide (OD) radicals at room temperature. Owing to the similar reactivity and low prevalence of deuterium in nature, OD radicals can be utilized to visualize the OH radical interactions with water and living cells. Escherichia coli in water were treated with OD radicals, and D atom incorporation into cells was visualized using time-of-flight SIMS and Nano-SIMS. The results show that D atoms from NTPJ reach the cytoplasm of E. coli in H 2 O, indicating the usefulness of this OD-tracking method for the study of radical interactions with living cells.
    Print ISSN: 0003-6951
    Electronic ISSN: 1077-3118
    Topics: Physics
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 73 (1993), S. 1011-1017 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A thermodynamic calculation is conducted to determine the distribution of hydrogen atoms absorbed in a one-dimensional palladium rod, along which a temperature gradient is imposed. The rod is assumed to be completely sealed, so that the total number of the hydrogen atoms is conserved regardless of its thermal conditions. A model calculation based on a zeroth-order interaction model predicts that upon the imposition of the gradient, the hydrogen distribution will significantly change from its initial uniformity. The profile of the redistributed hydrogen can be discontinuous at one point on the rod. The concentration gap developed at this point of temperature T implies that the two-phase region in the palladium hydride phase under the thermal equilibrium is nonexistent under the nonequilibrium condition. Similar to the disappearance of the two-phase region above the critical temperature, the concentration gap is reduced with an increase of the temperature gradient.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 69 (1991), S. 8056-8063 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Based on a theoretical estimation, a simple new scheme to generate high-dose, steady-state neutrons is presented. This new high-dose neutron source makes use of the plasma ion implantation, which consists of a cylindrical palladium rod saturated with deuterium atoms and immersed in a deuterium or tritium plasma. The plasma is contained by a grounded conducting chamber. The palladium rod is connected to a high-power modulator, which provides a series of negative-voltage pulses. During these negative pulses, deuterium or tritium ions fall into the palladium rod and collide with the deuterium atoms in it, initiating fusion reactions and emitting neutrons. This neutron device will be portable, reliable and almost free from radiation hazards except for the neutrons themselves. For reasonable system parameters allowed by present technology, we find from a theoretical calculation that the average neutron production rate 〈dN/dt〉 = 2.3 × 1012 s−1 for D-T reaction, which may be more than enough for most applications. The average neutron production rate for D-D reaction is two order of magnitude less than that for D-T reaction.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 72 (1992), S. 2549-2555 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A simple theoretical model is developed to explain neutron thermalization properties. Assuming that a fast neutron source is located at the origin, the neutron distribution is described as a function of distance from the source. Based on the continuous slowing-down model, it is shown that the thermal neutron flux is a simple function of the diffusion constant D, the reciprocal length κ, and the Fermi age τ of a moderator material. Several points are noteworthy from calculation of the thermal neutron flux. First, theoretical results for polyethylene moderator agree remarkably well with simulation data obtained from a Monte Carlo simulation. Second, the thermal neutron flux at the origin is proportional to the square of moderator density. Third, the volume of the large thermal neutron flux increases drastically as the Fermi age increases. Finally, we also note that theoretical results agree reasonably well with experimental data for a broad range of physical parameters. A simple analytical expression of the thermalization factor of a moderator material is derived and it is found that its theoretical values agree rather closely with measured values.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 91 (2002), S. 1031-1039 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A theoretical model of the plasmas generated by a sheath-helix antenna is developed for axisymmetric perturbations. The system configuration consists of a cylindrical plasma column inside a dielectric tube of radius Rc. The eigenvalue equation is obtained and the eigenfunction is identified as the Bessel function J0(x) of the first kind of order zero. The radial wave numbers ξ and η for the eigenfunction are described in terms of the rf frequency and plasma density. A full dispersion relation is analytically obtained, including influence of the finite plasma size, antenna effects, the electron inertia, and ion mass contributions. It is shown from the dispersion relation that the radial mode number ξ approaches infinity at the lower-hybrid frequency, exhibiting a resonance condition. Meanwhile, the radial wave number η approaches 3.83/Rc at the lower-hybrid frequency. A cross-sectional view of the light emission in experiment indicates that the helicon-plasma density at the lower-hybrid frequency has a hollow profile. The azimuthal component Eθ(r) of the perturbed electric field observed experimentally is very similar to the theoretical model of J1(3.83r/Rc) at the lower-hybrid frequency. The emission peak coincides with the radial location of the strongest electric-field intensity. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 89 (2001), S. 4224-4230 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Transient behavior of the cavity excitation caused by beam head effects in high-power klystrons is investigated. Recognizing that the input cavity in a klystron can be excited by the input microwave signal and the head of the electron beam, it is shown that the frequency of the induced cavity voltage Vs driven by the signal is the same as the signal frequency ωs. On the other hand, the frequency of the induced cavity voltage Vh driven by the beam head is the cavity resonance frequency ω0. An expression of the induced voltage Vh is obtained by making use of the Laplace transformation. The induced voltage Vh caused by the beam head is expressed in terms of the current-change profile in time. The magnitude of the induced voltage caused by the beam head is inversely proportional to the beam rise time, thereby decreasing drastically for a large value of the beam rise time. It is also shown that the beam head effects are completely negligible whenever the beam rise time τr satisfies ω0τr=2nπ where n is an integer. The amplitude of the whole cavity voltage oscillates with the beat wave frequency Δω=ω0−ωs. This amplitude oscillation may play an important role in pulsed high-power klystrons. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 9 (2002), S. 706-712 
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The influence of the Penning effects on the high-pressure discharge in a neon–xenon mixed gas was investigated in connection with applications to the plasma display panel. A theoretical model of the breakdown voltage in a mixed gas is developed, based on the Townsend criteria. The breakdown temperature Tb and voltage Vb are obtained in terms of the gas mixture ratio. As an example, electrical breakdown properties in neon gas mixed with xenon are investigated. It is shown that the electron breakdown temperature Tb decreases monotonically as the xenon mole fraction χ increases. The Penning effects modify the electron temperature significantly, particularly in the range of a small mole fraction. A preliminary experiment using the plasma display panel is carried out to verify some of the theoretical models. The Paschen curves of the breakdown voltage are experimentally obtained in terms of the pressure parameter (pd) and the xenon mole fraction. It is shown that the breakdown voltage is reduced significantly at the xenon mole fraction of χ=0.015, which agrees remarkably well with experimental data. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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