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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 33 (2002), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In this study Atlantic halibut (Hippoglossus hippoglossus L.) larvae (0.12 ± 0.04 g) were, from day 40 post first feeding, offered six diets in which 10% or 30% of the dietary protein was hydrolysed with (a) pepsin (P), (b) pepsin + trypsin (PT) or (c) pepsin + trypsin + chymotrypsin (PTC). In addition, a diet without hydrolysed protein was offered, and enriched Artemia was fed as control. The amount of soluble protein increased progressively with the enzyme treatments P, PT and PTC and with higher inclusion levels of hydrolysed protein. Survival was highest among the larvae offered Artemia (83 ± 0%) or the diet 10P (10% pepsin hydrolysed protein; 67 ± 4%). The diet 10P supported survival significantly better than the more hydrolysed diets 10PTC, 30P, 30PT and 30PTC, but not significantly better than the non-hydrolysed diet and 10PT. Specific growth rate (SGR) was 1.76 ± 0.20 in average for all groups of larvae and was not significantly affected by the diets. Still, the larvae offered pepsin hydrolysed diets tended to have better growth (2.10 ± 0.05 SGR; P 〈 0.06) than the larvae offered the other hydrolysed diets. The larvae offered the formulated diets did not differ in chemical composition.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 31 (2000), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Atlantic salmon smolt (64 g) were fed 40, 300 or 1100 mg kg−1 all-rac-α-tocopheryl acetate feed under normoxic and moderate hyperoxic conditions for 12 weeks. After 7 weeks, however, the hyperoxic fish were subjected to a temporary severe supersaturation of dissolved oxygen with development of gas bubble disease and substantial mortality as a result. Liver α-tocopherol (α-TOH) levels reflected the dietary input after 6 and 12 weeks of feeding. Increased levels of thiobarbituric-reactive substances (TBARS) in the liver of hyperoxygenated fish after 6 weeks indicate that moderate hyperoxic conditions may represent an oxidative challenge to the fish. After 12 weeks, liver ascorbic acid (AA) content was reduced in hyperoxygenated fish, and a decreasing tendency was also found in the liver content of α-TOH. Effects of oxygen supersaturation were also observed on liver antioxidant enzyme activities, content of glutathione and total mercapthans in liver, phagocytic chemiluminescent response and total plasma protein level, indicating a disturbed redox balance. No effects of dietary vitamin E were observed on any of the measured parameters, indicating that high levels of dietary vitamin E under these circumstances did not protect the fish against oxidative stress.
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  • 3
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The influence of α-tocopheryl acetate (α-TOAc) on plasma concentration and fillet deposition ofdietary astaxanthin was investigated in Atlantic salmon Salmo salar L. The diets were added 30 or 50 mg kg–1 astaxanthin, and 200, 400 or 800 mg kg–1α-TOAc at each astaxanthin level. Improved flesh deposition of astaxanthin by 8–14% was achieved for fish fed diets with 30 and 50 mg kg–1 astaxanthin, respectively, by the dietary addition of 800 compared with 200 mg kg–1α-TOAc. These results were supported by CIE[1976]L*a*b* tristimulus redness measurements (a* value). Plasma astaxanthin concentration mirrored the muscle astaxanthin concentration in the groups of fish fed a diet containing 30 mg kg–1 astaxanthin. The salmon fed a high astaxanthin and low α-TOAc diet had the highest plasma concentration of idoxanthin (P 〈 0.05). Astaxanthin retention was significantly higher (P 〈 0.001) in salmon fed 30 mg kg–1 astaxanthin than in those fed 50 mg kg–1 astaxanthin, but was not significantly affected by dietary α-TOAc. Liver weight, body weight, specific growth rate, feed/gain ratio and mortalities were not affected by dietary α-TOAc levels. In conclusion, the dietary addition of α-TOAc appears to increase astaxanthin fillet deposition in salmonids and may reduce the demand for astaxanthin supplementation. The effect was rather small and requires verification.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 26 (1995), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The minimum requirement of vitamin E for Atlantic salmon, Salmo salar L., at first feeding was found to be 60 mg dl-α-tocopheryl acetate per kg dry diet. The dry diet contained 5.6% polyunsaturated fatty acids, which is similar to that used in practical diets at first feeding. Preliminary results indicate that the optimal dietary vitamin E level is 120 mg dl-α-tocopheryl acetate per kg dry diet, or more. Further research is necessary to confirm this hypothesis.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 32 (2001), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Small raceways were used in a weaning experiment with Atlantic halibut (Hippoglossus hippoglossus L.) larvae. The size of the tanks was 1.0 × 0.4 m with a 1- to 2-cm water level. Duplicate larval groups were transferred to the raceways from circular first feeding tanks at 0.07, 0.10 and 0.16 g wet weight, while recommended weaning size of this species is 0.2–0.3 g. During the first 7 days of weaning, Artemia was used as a food supplement in combination with the formulated dry feed. Thereafter only dry feed was used. The dry feed used in this experiment was produced by a special heat technique. The 0.07, 0.10 and 0.16 g larval groups were evaluated after 31, 25 and 17 days respectively (same date) The corresponding average survival was 81.4%, 78.0% and 96.6% and the specific daily growth rate was 3.18%, 3.17% and 2.38% respectively. In the Artemia control group, a survival rate of 96.0% and a growth rate of 5.28% was achieved. To evaluate the weaning success, the groups were followed in a 22-day post-weaning period on a commercial dry diet. Higher growth rates, 5.8–6.9%, were then obtained in all experimental groups, except control. The survival here averaged approximately 80% in the three experimental groups, but showed some differences between replicates. One hundred per cent survival was achieved during weaning in the former Artemia group. From the start of weaning to the end of the post-weaning period, the survival rates averaged 64% for the 0.07 and 0.10 g groups, approximately 80% for the 0.16 g group and 96% in the Artemia control group. Higher variance (CV) through the experiment and highest growth of the 75% quartiles of the fish groups compared with the 50% and 25%, indicated suppressed growth of the smaller fish. The successful weaning at these small sizes considerably reduced the live food period. Based on the present knowledge of the energetic demands of this species, it is calculated that weaning at 0.07 g compared with 0.25 g will reduce the amount of Artemia needed by at least 60%. No differences in pigmentation or degree of completed eye migration were detected between groups, indicating that this is determined at earlier developmental stages.
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  • 6
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The iodine concentration in wild zooplankton was 700 times higher than in Artemia and threefold higher in Atlantic halibut Hippoglossus hippoglossus larvae fed wild zooplankton than in those fed Artemia. In larvae fed wild zooplankton thyroxine (T4) and triiodothyronine (T3) concentrations were significantly higher than in those fed Artemia at the commencement of metamorphosis, 68–77 days post hatching. There was no difference in the amino acids phenylalanine and tyrosine levels in larvae fed Artemia in comparison with those fed wild zooplankton. The selenium level was significantly higher in larvae fed Artemia than in those fed wild zooplankton, but concentrations in the prey were not different. The results indicate sufficient amounts of phenylalanine, tyrosine and selenium in Artemia, but indicated a lower thyroid status due to an insufficient iodine supply at commencement of metamorphosis in larvae fed Artemia. This may partly explain the higher frequency of juveniles with complete eye migration and asymmetric pigmentation in the group fed wild zooplankton.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 55 (1999), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ascorbic acid (AA) and α-tocopherol (α-TOH) levels in whole Atlantic halibut larvae were constant during the yolk sac stage at 170 and 131 ng individual−1, respectively. At hatching c. 80% of the AA and 97% of the α-TOH were contained within the yolk-sac compartment. With development, AA and α-TOH levels in the yolk decreased, at different rates. At first feeding (at 200 day degrees post hatch, D°PH)〉95% of AA but 〈30% of α-TOH in the yolk at hatching had been transferred to the larval body. Transfer of α-TOH was completed at 360 D°PH, when the yolk was completely absorbed. The plankton offered to the larvae at first feeding (chiefly Temora longicornis) contained 756 μg g−1 AA and 120 μg g−1α-TOH (dry weight). The AA content increased to 472 ng individual−1 within one week after first feeding, while it declined slightly in unfed larvae. In fed larvae the AA content reached c. 3500 ng individual−1 at 580 D)PH. The α-TOH content increased only slightly in the first week of feeding (206 to 431 D°PH), but then increased to 〉 800 ng individual−1 at 483 D°PH.
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  • 8
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The development of cataracts in Atlantic salmon, Salmo salar L., was studied in 16 groups of smolts fed diets differing in prooxidant (iron, copper, manganese) and antioxidant (vitamin E, vitamin C, astaxanthin) composition and lipid level for 23 weeks in sea water, using a 27--3 reduced factorial design. The seven dietary variables were systematically varied at low (requirement level and 150 g lipid kg−1) and high levels (below known toxic levels and 320 g lipid kg−1). A mean endpoint cataract incidence of approximately 36% was observed. High dietary levels of vitamin C and astaxanthin reduced cataract frequency, whereas high dietary lipid level, iron and manganese were associated with increased cataract frequencies. Considering the nutritional status of selected organs of the fish, only the status of ascorbic acid correlated negatively to cataract development (P 〈 0.05). The lens glutathione (GSH) status was not correlated to cataract frequency, nor statistically explained by the dietary variables. However, the study shows that balancing the diet with respect to pro- and antioxidant nutrients may significantly protect Atlantic salmon against development of cataracts. An incidence of reversible osmotic cataract observed at week 14 was positively correlated to plasma glucose concentration.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture nutrition 9 (2003), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fish fillet quality has been shown to be influenced by the level of antioxidants in preslaughter diet. Thus, an experiment was conducted to study the effect of different levels of vitamin E and C on the fillet quality of market-size reared turbot (Scophthalmus maximus). Turbot of a mean initial weight of 347 ± 20 g were divided into four groups and fed commercial turbot diets (60% protein, 12% fat), supplemented with α-tocopheryl acetate (mg kg−1) and ascorbyl-2 monophosphate (mg kg−1) at the following dietary levels: 500/100, 1000/100, 100/1000, 100/100 respectively. Over a dietary supplementation period of 15 weeks, fish were fed to satiation and reached a final mean weight of 916 ± 29 g. α-Tocopherol levels increased significantly (P 〈 0.001) in tissue (i.e. muscle, liver, heart and kidney) of fish fed diets containing elevated levels of α-tocopheryl acetate. In ice storage, fillets of these fish exhibited significantly lower (P 〈 0.001) levels of lipid oxidation, and showed significantly less (P 〈 0.001) colour deterioration (higher hue angle and lower chroma). Elevated dietary α-tocopheryl acetate levels had a negative effect (P ≤ 0.001) on the concentration of ascorbic acid in muscle tissue. An increase in dietary vitamin C did not have any detectable effect on fillet quality. Prolonged feeding times had a negative effect on lipid oxidation (P 〈 0.001) and colour deterioration (P 〈 0.01). These results suggest that increased dietary α-tocopheryl acetate could prevent colour deterioration and lipid oxidation of turbot fillets in retail storage on ice.
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  • 10
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Atlantic halibut larvae were fed docosohexanoic acid- (DHA) selco enriched Artemia (RH-cysts) or wild zooplankton in duplicate tanks from first-feeding and 60 days onward. The zooplankton were collected from a fertilized sea water pond and consisted mainly of different stages of Eurytemora affinis and Centropages hamatus. There were no differences in survival, or in growth during the first 45 days of feeding, between larvae fed the two prey items, but the larvae fed Artemia showed much higher incidence of malpigmentation and impaired eye migration than larvae fed zooplankton. The prey organisms contained similar amounts of dry matter and protein, but Artemia was higher in lipid and glycogen than the zooplankton. Larvae fed Artemia were higher in both glycogen and lipid than the zooplankton-fed larvae towards the end of the feeding period. There were large differences between the prey organisms in the concentrations of essential fatty acids (% of total fatty acids) which was reflected in the fatty acid composition of the larval body. It is concluded that the macronutrient composition of Artemia in the present study was probably within the optimal range for promotion of growth and survival in young Atlantic halibut. The concentration of n-3 HUFA, and especially DHA, is however, very much lower in enriched Artemia than in copepods, and may be one of the factors triggering developmental errors in Atlantic halibut.
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