ISSN:
1432-1424
Keywords:
lymphocyte-liposome interaction
;
vesicle-cell fusion
;
endocytosis
;
cellular vesicle adsorption
;
membrane-bound spin probe
;
cholestane spin label
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary When lecithin-cholesterol vesicles, containing the membrane-bound spin probe 3-doxyl-cholestane, were set in contact with mouse lymphocytes, the vesicles adsorbed to the cell and vesicle-membrane components were transferred to it. The spin probe was enzymatically reduced at the inside of the cell membrane. The spin-label method provided a means to determine quantitatively the extent of vesicles adsorption and vesicle-cell fusion by measuring the transfer of vesicles membrane material to the cell. This method, together with the reduction of spin label by the cell, allowed also a quantitative estimate of the extent of endocytosis during cell-liposome interaction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01870881
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