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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 8 (1985), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. A comparative study of immunological methods for detecting infectious haematopoietic necrosis virus (IHNV) was made. The anti–IHNV antibody titre was measured by solid phase direct binding assays with‘125iodinated Protein A from Staphylococcus aureus and with immunoperoxidase staining. The binding antibody titre was much higher than that obtained in the virus neutralization assay. The high binding antibody titre of rabbit anti–IHNV sera made the development of two immunological tests for IHNV possible. Virus–specific proteins were detected on nitrocellulose membranes after transfer from denaturing polyacrylamide gels. The immunological methods were highly specific, sensitive lo less than 10 ng of virus protein, and were useful in characterizing the different strains of IHNV.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: An investigation of virus-specific protein maturation in infectious pancreatic necrosis virus (IPNV) infected Chinook salmon embryo cells (CHSE-214) was undertaken. The precursor protein (pVP2–1) of the major mature capsid protein (VP2) was processed sequentially from pVP2–1 to pVP2–2 and VP2. Experiments using serine proteinase inhibitors showed that the maturation of the VP2 was blocked in the pVP2–1 post-translational cleavage steps. A protinin, a potent proteinase inhibitor, at 800 μg ml–1 blocked pVP2–2 to VP2 and the cleavage of VP4 (28 kDa) to VP4–1 (25 kDa). Therefore, our data showed that the maturation of the capsid protein (VP2) and cleavage of VP4 (NS proteinase) can be blocked by serine proteinase inhibitors.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 21 (1998), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Infectious pancreatic necrosis virus (IPNV) causes an acute, contagious disease in a number of economically important fish species (Pilcher & Fryer 1980). It is a member of the Birnaviridae (Brown 1986). In this study, the present authors first characterized the process of fish cell damage caused by IPNV infection in cultured cells, and found that it induced fragmentation of chromosomal DNA into oligonucleosomes and also caused nuclear fragmentation by secondary necrosis.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 16 (1993), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Six isolates of infectious pancreatic necrosis virus (IPNV) were obtained from trout or eel farms in Taiwan in 1987. By using the electrophoretic analysis of 35S-methionine-labelled early viral polypeptide patterns and silver-stained ds RNA gel patterns, a comparison of these isolates with the three archetypal IPNV serotypes (AB, SP and VR-299) was made. They were all identical with VR-299, both in the RNA and early viral polypeptide patterns. From the results obtained, it was apparent that the VR-299 serotype of IPNV was just as widespread in eel as in trout in Taiwan. This is the first case of VR-299 isolation from eel.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Structural and multidisciplinary optimization 7 (1994), S. 199-205 
    ISSN: 1615-1488
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In a shell structure, the discontinuity at the intersection of two shells causes stress concentration. This paper presents a procedure which couples the Curvature Function Method with the FAST1 structural shell analysis program to find a fully stressed thickness profile which keeps the stress at the discontinuities at the nominal stress value. The Curvature Function Method is a zero-order method that requires only stress values along the shell, not gradients of the stresses with respect to the design variables, and the resulting thickness profile has C2 continuity. Although the method is independent of the structural analysis program used to determine stress values, Fast1 provides a particular advantage because it allows the user to model complex shells with only a few large shell elements and still retain a sufficiently accurate solution. Thus both preparation and computation times are reduced substantially. Convergence of different initial designs to one final design using this procedure is demonstrated for a cylinder-cone intersection problem. This procedure is also applied to two other shell models with multiple discontinuities to find their fully stressed thickness profiles. The procedure presented in this paper provides a practical technique and tool to aid the design engineer, although the fully stressed design may not be the theoretically optimal design of minimum weight.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2015-06-17
    Description: The human JC virus (JCV) is potentially carcinogenic to humans as a Group 2B carcinogen, and it is ubiquitous in human populations. To investigate whether the small tumour (ST) antigen of the JCV contributes to genomic instability, we established cell lines stably expressing the JCV ST and examined its role in DNA repair. Results from host cell reactivation (HCR) assay revealed that the established cell lines exhibited lower nucleotide excision repair (NER) activity than the vector control cells did. The presence of -H2AX, a marker of DNA damage, indicated that the established cell line contained more DNA damage foci compared with vector control cells. Furthermore, the results of clonogenic analyses indicated that the JCV ST-expressing cells were more sensitive than the vector control cells to ultraviolet (UV) irradiation and cisplatin treatment. Micronuclei formation assay revealed that the JCV ST-positive cells presented more chromosomal breakages than did the JCV ST-negative cells, particularly after exposure to DNA-damaging agents. The xeroderma pigmentosum Group D protein, a DNA helicase involved in NER, was downregulated in the JCV ST-positive cells in response to UV irradiation. The effect of the protein phosphatase 2A (PP2A) inhibitor okadaic acid on NER was similar to that of the ST, which is a PP2A-binding protein. Therefore, the deactivation of the PP2A might underlie ST-mediated NER inhibition. The results of this study indicate that exposing JCV ST-positive cells to DNA-damaging agents causes genomic instability, which contributes to carcinogenesis. Our data provide further evidence on the association between the JCV ST and human cancer.
    Print ISSN: 0267-8357
    Electronic ISSN: 1464-3804
    Topics: Biology , Medicine
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  • 7
    Publication Date: 2001-10-11
    Print ISSN: 0090-4341
    Electronic ISSN: 1432-0703
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Published by Springer
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