ISSN:
1617-4623
Keywords:
Transcriptional activation
;
DNA replication
;
λdv plasmid
;
Downstream of λori
;
lac promoter
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary DNA replication of lambda phage depends on transcriptional activation at or around the λori region by RNA polymerase. To elucidate the function of the transcriptional activation, we constructed several plasmids carrying λori and lacP, whose relative locations and directions were different from each other, and studied replication activity of these recombinant plasmids. Transcription in a region immediately downstream from λori, but not in the λori region, was found to be essential for plasmid replication. Transcription proceeding over a certain minimal length was required and only rightward-directed transcription was effective for the activation
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00332239
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