Publication Date:
1994-07-01
Description:
Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gu, H -- Marth, J D -- Orban, P C -- Mossmann, H -- Rajewsky, K -- New York, N.Y. -- Science. 1994 Jul 1;265(5168):103-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Genetics, University of Cologne, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8016642" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
DNA Nucleotidyltransferases/genetics/metabolism
;
DNA Polymerase I/*genetics/metabolism
;
Female
;
*Gene Deletion
;
Genetic Engineering/*methods
;
Homozygote
;
*Integrases
;
Male
;
Mice
;
Mice, Knockout
;
Mice, Transgenic
;
Mutation
;
Recombination, Genetic
;
Stem Cells/enzymology
;
T-Lymphocytes/*enzymology
;
Transfection
;
*Viral Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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