Publication Date:
1989-03-17
Description:
Human endothelial cells produced a neutrophil chemotactic factor (NCF) upon stimulation with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), or lipopolysaccharide (LPS). The expression of endothelial cell-derived NCF messenger RNA and biological activity was both time- and concentration-dependent. Maximal NCF mRNA expression occurred at 10 and at 2 nanograms per milliliter for TNF and IL-1 beta, respectively; mRNA expression was first observed 1 hour after stimulation and was maintained for at least 24 hours. In situ hybridization analysis showed that NCF mRNA peaked in treated cells by 24 hours, whereas unstimulated cells were negative. These studies demonstrated that endothelial cells may participate in neutrophil-mediated inflammation by synthesizing a chemotactic factor in response to specific monokines and LPS.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Strieter, R M -- Kunkel, S L -- Showell, H J -- Remick, D G -- Phan, S H -- Ward, P A -- Marks, R M -- HL31237/HL/NHLBI NIH HHS/ -- HL31936/HL/NHLBI NIH HHS/ -- HL35276/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1989 Mar 17;243(4897):1467-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2648570" target="_blank"〉PubMed〈/a〉
Keywords:
Blotting, Northern
;
Chemotactic Factors/*genetics
;
Endothelium, Vascular/*physiology
;
Gene Expression Regulation/drug effects
;
Humans
;
In Vitro Techniques
;
Interleukin-1/*pharmacology
;
Interleukin-8
;
Lipopolysaccharides/*pharmacology
;
Oligonucleotide Probes
;
Time Factors
;
Tumor Necrosis Factor-alpha/*pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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