Publication Date:
1997-09-26
Description:
DNA-(cytosine-5) methyltransferase (MCMT) methylates newly replicated mammalian DNA, but the factors regulating this activity are unknown. Here, MCMT is shown to bind proliferating cell nuclear antigen (PCNA), an auxiliary factor for DNA replication and repair. Binding of PCNA requires amino acids 163 to 174 of MCMT, occurs in intact cells at foci of newly replicated DNA, and does not alter MCMT activity. A peptide derived from the cell cycle regulator p21(WAF1) can disrupt the MCMT-PCNA interaction, which suggests that p21(WAF1) may regulate methylation by blocking access of MCMT to PCNA. MCMT and p21(WAF1) may be linked in a regulatory pathway, because the extents of their expression are inversely related in both SV40-transformed and nontransformed cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chuang, L S -- Ian, H I -- Koh, T W -- Ng, H H -- Xu, G -- Li, B F -- New York, N.Y. -- Science. 1997 Sep 26;277(5334):1996-2000.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Chemical Carcinogenesis Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge Crescent, Singapore 119260, Republic of Singapore.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9302295" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Cell Line, Transformed
;
Cell Nucleus/metabolism
;
Cyclin-Dependent Kinase Inhibitor p21
;
Cyclins/chemistry/*metabolism
;
DNA (Cytosine-5-)-Methyltransferase/*metabolism
;
DNA Damage
;
*DNA Methylation
;
DNA Repair
;
DNA Replication
;
Humans
;
Molecular Sequence Data
;
Peptides/pharmacology
;
Proliferating Cell Nuclear Antigen/*metabolism
;
Recombinant Fusion Proteins/metabolism/pharmacology
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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