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  • 1
    Publication Date: 2015-04-28
    Description: Monotremes (echidnas and platypus) retain an ancestral form of reproduction: egg-laying followed by secretion of milk onto skin and hair in a mammary patch, in the absence of nipples. Offspring are highly immature at hatching and depend on oligosaccharide-rich milk for many months. The primary saccharide in long-beaked echidna milk is an acidic trisaccharide Neu4,5Ac 2 (α2–3)Gal(β1–4)Glc (4- O -acetyl 3'-sialyllactose), but acidic oligosaccharides have not been characterized in platypus milk. In this study, acidic oligosaccharides purified from the carbohydrate fraction of platypus milk were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 1 H-nuclear magnetic resonance spectroscopy. All identified structures, except Neu5Ac(α2–3)Gal(β1–4)Glc (3'-sialyllactose) contained Neu4,5Ac 2 (4- O -acetyl-sialic acid). These include the trisaccharide 4- O -acetyl 3'-sialyllactose, the pentasaccharide Neu4,5Ac 2 (α2–3)Gal(β1–4)GlcNAc(β1–3)Gal(β1–4)Glc (4- O -acetyl-3'-sialyllacto- N -tetraose d) and the hexasaccharide Neu4,5Ac 2 (α2–3)Gal(β1–4)[Fuc(α1–3)]GlcNAc(β1–3)Gal(β1–4)Glc (4- O -acetyl-3'-sialyllacto- N -fucopentaose III). At least seven different octa- to deca-oligosaccharides each contained a lacto- N -neohexaose core (LN n H) and one or two Neu4,5Ac 2 and one to three fucose residues. We conclude that platypus milk contains a diverse (≥20) array of neutral and acidic oligosaccharides based primarily on lactose, lacto- N -neotetraose (LN n T) and LN n H structural cores and shares with echidna milk the unique feature that all identified acidic oligosaccharides (other than 3'-sialyllactose) contain the 4- O -acetyl-sialic acid moiety. We propose that 4-O-acetylation of sialic acid moieties protects acidic milk oligosaccharides secreted onto integumental surfaces from bacterial hydrolysis via steric interference with bacterial sialidases. This may be of evolutionary significance since taxa ancestral to monotremes and other mammals are thought to have secreted milk, or a milk-like fluid containing oligosaccharides, onto skin surfaces.
    Print ISSN: 0959-6658
    Electronic ISSN: 1460-2423
    Topics: Biology , Medicine
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  • 2
    Publication Date: 2015-06-10
    Description: The supply of inorganic carbon (Ci; CO2 and HCO3–) is an environmental rate-limiting factor in aquatic photosynthetic organisms. To overcome the difficulty in acquiring Ci in limiting-CO2 conditions, an active Ci uptake system called the CO2-concentrating mechanism (CCM) is induced to increase CO2 concentrations in the chloroplast stroma. An ATP-binding...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 3
    Publication Date: 2015-12-19
    Description: In order to achieve a wide refrigerating temperature range in magnetic refrigeration, it is effective to layer multiple materials with different Curie temperatures. It is crucial to have a detailed understanding of physical properties of materials to optimize the material selection and the layered structure. In the present study, we discuss methods for estimating a change in physical properties, particularly the Curie temperature when some of the Gd atoms are substituted for non-magnetic elements for material design, based on Gd as a ferromagnetic material which is a typical magnetocaloric material. For this purpose, whilst making calculations using the S =7/2 Ising model and the Monte Carlo method, we made a specific heat measurement and a magnetization measurement of Gd-R alloy (R = Y, Zr) to compare experimental values and calculated ones. The results showed that the magnetic entropy change, specific heat, and Curie temperature can be estimated with good accuracy using t...
    Print ISSN: 1757-8981
    Electronic ISSN: 1757-899X
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 4
    Publication Date: 2015-12-19
    Description: Currently, many space missions using cryogenic temperatures are being planned. In particular, high resolution sensors such as Transition Edge Sensors need very low temperatures, below 100 mK. It is well known that the adiabatic demagnetization refrigerator (ADR) is one of most useful tools for producing ultra-low temperatures in space because it is gravity independent. We studied a continuous ADR system consisting of 4 stages and demonstrated it could provide continuous temperatures around 100 mK. However, there was some heat leakage from the power leads which resulted in reduced cooling power. Our efforts to upgrade our ADR system are presented. We show the effect of using the HTS power leads and discuss a cascaded Carnot cycle consisting of 2 ADR units.
    Print ISSN: 1757-8981
    Electronic ISSN: 1757-899X
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 5
    Publication Date: 2014-10-14
    Description: Author(s): Lei Shu, D. E. MacLaughlin, C. M. Varma, O. O. Bernal, P.-C. Ho, R. H. Fukuda, X. P. Shen, and M. B. Maple The formation of heavy-fermion bands can occur by means of the conversion of a periodic array of local moments into itinerant electrons via the Kondo effect and the huge consequent Fermi-liquid renormalizations. Leggett predicted for liquid He3 that Fermi-liquid renormalizations change in the superc... [Phys. Rev. Lett. 113, 166401] Published Mon Oct 13, 2014
    Keywords: Condensed Matter: Electronic Properties, etc.
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
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  • 6
    Publication Date: 2014-06-04
    Description: Identification of carbohydrate sequences that determine affinity to specific chemokines is a critical step for strategies to interfere with chemokine-mediated leukocyte trafficking. Here, we first characterized the development of allergic asthma in Tie2-dependent and inducible Ext1-knockout (Tie2-Ext1iKO) mice. We showed that heparan sulfate is essential for leukocyte recruitment in the...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 7
    Publication Date: 2014-04-22
    Description: Author(s): D. E. MacLaughlin, P.-C. Ho, Lei Shu, O. O. Bernal, Songrui Zhao, A. A. Dooraghi, T. Yanagisawa, M. B. Maple, and R. H. Fukuda Muon spin rotation and relaxation (μSR) experiments have been carried out to characterize magnetic and superconducting ground states in the Pr1−xNdxOs4Sb12 alloy series. In the ferromagnetic end compound NdOs4Sb12 the spontaneous local field at positive-muon (μ+) sites below the ordering temperature... [Phys. Rev. B 89, 144419] Published Mon Apr 21, 2014
    Keywords: Magnetism
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
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  • 8
    Publication Date: 2006-08-12
    Description: The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell-specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12-amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3. These results suggest that the functional peptide of CLV3 is MCLV3.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kondo, Tatsuhiko -- Sawa, Shinichiro -- Kinoshita, Atsuko -- Mizuno, Satoko -- Kakimoto, Tatsuo -- Fukuda, Hiroo -- Sakagami, Youji -- New York, N.Y. -- Science. 2006 Aug 11;313(5788):845-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16902141" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Motifs ; Amino Acid Sequence ; Arabidopsis/*cytology/genetics/metabolism ; Arabidopsis Proteins/*chemistry/genetics/*metabolism/pharmacology ; Cell Differentiation ; Cells, Cultured ; Hydroxyproline/chemistry ; Meristem/*cytology/drug effects/metabolism ; Molecular Sequence Data ; Oligopeptides/chemistry/*metabolism/pharmacology ; Plant Roots/growth & development ; Plants, Genetically Modified ; Signal Transduction ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Stem Cells/cytology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
    Publication Date: 2006-08-12
    Description: In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay of single-cell transdifferentation demonstrates that a dodecapeptide with two hydroxyproline residues is the functional product of genes from the CLE family, which includes CLAVATA3 in Arabidopsis. The dodecapeptide suppresses xylem cell development at a concentration of 10(-11) M and promotes cell division. An application, corresponding to all 26 Arabidopsis CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ito, Yasuko -- Nakanomyo, Ikuko -- Motose, Hiroyasu -- Iwamoto, Kuninori -- Sawa, Shinichiro -- Dohmae, Naoshi -- Fukuda, Hiroo -- New York, N.Y. -- Science. 2006 Aug 11;313(5788):842-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16902140" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Arabidopsis/cytology ; Arabidopsis Proteins/chemistry/metabolism ; Asteraceae/*cytology ; Base Sequence ; Biological Assay ; Cell Communication ; *Cell Differentiation ; Cells, Cultured ; Ligands ; Meristem/cytology ; Molecular Sequence Data ; Oligopeptides/chemistry/isolation & purification/*metabolism/pharmacology ; Plant Proteins/chemistry/*metabolism ; Plant Roots/cytology/growth & development ; Plant Structures/*cytology ; *Signal Transduction ; Stem Cells/*cytology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
    Publication Date: 2012-09-18
    Description: A specifically patterned cell wall is a determinant of plant cell shape. Yet, the precise mechanisms that underlie initiation of cell wall patterning remain elusive. By using a reconstitution assay, we revealed that ROPGEF4 (Rho of plant guanine nucleotide exchange factor 4) and ROPGAP3 [ROP guanosine triphosphatase (GTPase)-activating protein 3] mediate local activation of the plant Rho GTPase ROP11 to initiate distinct pattern of secondary cell walls in xylem cells. The activated ROP11 recruits MIDD1 to induce local disassembly of cortical microtubules. Conversely, cortical microtubules eliminate active ROP11 from the plasma membrane through MIDD1. Such a mutual inhibitory interaction between active ROP domains and cortical microtubules establishes the distinct pattern of secondary cell walls. This Rho-based regulatory mechanism shows how plant cells initiate and control cell wall patterns to form various cell shapes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Oda, Yoshihisa -- Fukuda, Hiroo -- New York, N.Y. -- Science. 2012 Sep 14;337(6100):1333-6. doi: 10.1126/science.1222597.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. oda@biol.s.u-tokyo.ac.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22984069" target="_blank"〉PubMed〈/a〉
    Keywords: Arabidopsis/chemistry/*cytology/enzymology ; Arabidopsis Proteins/chemistry/genetics/*metabolism ; *Cell Shape ; Cell Wall/*chemistry/enzymology ; GTPase-Activating Proteins/chemistry/genetics/*metabolism ; Guanine Nucleotide Exchange Factors/chemistry/genetics/*metabolism ; Microtubule-Associated Proteins/chemistry/genetics/*metabolism ; Microtubules/chemistry/*enzymology ; Protein Structure, Tertiary ; Xylem/chemistry/*cytology/enzymology ; rho GTP-Binding Proteins/chemistry/genetics/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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