Publication Date:
2004-05-01
Description:
Members of the DExH/D superfamily of nucleic acid-activated nucleotide triphosphatases are essential for virtually all aspects of RNA metabolism, including pre-messenger RNA splicing, RNA interference, translation, and nucleocytoplasmic trafficking. Physiological substrates for these enzymes are thought to be regions of double-stranded RNA, because several DExH/D proteins catalyze strand separation in vitro. These "RNA helicases" can also disrupt RNA-protein interactions, but it is unclear whether this activity is coupled to duplex unwinding. Here we demonstrate that two unrelated DExH/D proteins catalyze protein displacement independently of duplex unwinding. Therefore, the essential functions of DExH/D proteins are not confined to RNA duplexes but can be exerted on a wide range of ribonucleoprotein substrates.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fairman, Margaret E -- Maroney, Patricia A -- Wang, Wen -- Bowers, Heath A -- Gollnick, Paul -- Nilsen, Timothy W -- Jankowsky, Eckhard -- R01 GM067700/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Apr 30;304(5671):730-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15118161" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/metabolism
;
Bacterial Proteins/metabolism
;
Binding Sites
;
Cell Cycle Proteins/*metabolism
;
DEAD-box RNA Helicases
;
Exons
;
Fungal Proteins/*metabolism
;
Nucleic Acid Conformation
;
Protein Binding
;
RNA/chemistry/metabolism
;
RNA Helicases/*metabolism
;
RNA Precursors/chemistry/*metabolism
;
RNA Splicing
;
RNA, Double-Stranded/chemistry/*metabolism
;
RNA, Messenger/chemistry/*metabolism
;
RNA-Binding Proteins/metabolism
;
Ribonucleoproteins/*metabolism
;
Transcription Factors/metabolism
;
Vaccinia virus/enzymology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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