ISSN:
1573-5036
Keywords:
autoradiography
;
freeze-cutting
;
maize
;
P-accumulation
;
P-depletion
;
phytate-P
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Autoradiographs of soil slices mapping the distribution of phytate-derived 33P around the primary root of 6-day-old maize seedlings were used to investigate the uptake of phytate by the root. Analysis of the autoradiographs with a laser densitometer and processing of the data with image analysing software resulted in a resolution of 40 μm. The effect of 33P-crossfire was corrected by analysis of the apparent 33P-gradient around a phosphate-impermeable teflon tube that was inserted into the labeled soil as a standard. In spite of the high resolution achieved, a significant depletion zone could not be detected when the soil was equilibrated with 33P-phytate. However, with 33P-inorganic phosphate, 2 concentric zones were obvious. Within the inner zone, P was accumulated by about 20%, while in the outer zone a corresponding depletion of P could be detected. The accumulation zone coincided with the extension of the root hair cylinder, whereas the depleted area was clearly beyond the range of the root hairs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00038742
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