ISSN:
1573-4838
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
Abstract A qualitative in vitro evaluation of poly(caprolactone) (PCL), poly(hydroxybutyrate) (PHB) and a poly(hydroxybutyrate)-(hydroxyvalerate) (PHB-PHV) copolymer was carried out using primary human osteoblasts (HOB) and a human osteosarcoma (HOS) cell line. The cells were grown on films of these polymers and cultured for 2 and 4 days with cells grown on Thermanox as a control. The cells on each of the polymers exhibited different cellular morphologies with different rates of cell proliferation. Results from a preliminary degradation study demonstrated that biodegradable materials can be partially degraded using enzymes such as papain and trypsin. Of the solutions tested, papain caused the greatest degradation, with phosphate-buffered saline (PBS) a physiological buffer having very little effect over a six week period. The bone cells were grown on partially degraded polymers and no differences in the performance of HOS and HOB cells on the materials were observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00213135
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