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  • 1
    Digitale Medien
    Digitale Medien
    Variation among progenies of diploid plants regenerated from haploid, microspore-derived cell-suspension protoplasts of rice (Oryza sativa L.) (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Progenies of 110 diploid R0 plants regenerated from haploid protoplasts derived from microspore callus cell suspension culture of rice (cv. Miara) were evaluated for 6 traits, along with control lines, in a replicated field trial. Complementary post-harvest observations were carried out for four panicle or grain characters. A wide range of variation was observed for all traits under study among the protoclonal lines. The mean of the protoclonal population was significantly different from that of the control for most traits and 64.5% of the lines differed from the control for at least one field-evaluated trait. A unidirectional shift towards later-maturing, taller, and lower-tillering lines was observed. Of all the lines, 39% were taller than the control by at least 10 cm, 31 % had flowering delayed by at least 5 days, and 13% exhibited significantly longer panicles (P = 0.05). As to the agronomical value of the lines, the variation resulted in a global negative drift although favourable variations were found in a few lines. All but two lines were homogeneous, suggesting that most variations existed in a homozygous form in the R0 plants. R2 lines grown from seeds of 17 R1 protoclonal lines stably inherited the changes and did not segregate, thereby confirming that variations are fixed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00780.x
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Flow cytometric determination of ploidy of plants regenerated from rice (Oryza sativa L. cv. miara) haploid tissue and protplast cultures
    Amsterdam : Elsevier
    Biology of the Cell 76 (1992), S. 275 
    Details
    ISSN: 0248-4900
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0248-4900(92)90394-G
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Transformation of haploid, microspore-derived cell suspension protoplasts of rice (Oryza sativa L.) (1996)
    Springer
    Plant cell reports 15 (1996), S. 766-770 
    Details
    ISSN: 1432-203X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We compared the transient activity of three cereal gene-derived promoter-gus fusions and the efficiency of selection mediated by three different selectable genes in a polyethylene glycol transformation system with haploid cell suspension protoplasts of rice. The maize ubiquitin promoter was found to be the most active in transformed protoplasts, and selection on ammonium glufosinate mediated by the bar gene was the most efficient for producing resistant calluses. Cotransformation of protoplasts with two separate plasmids carrying the gus and the bar genes, at either a 2∶1 or 1∶1 ratio, led to 0.8 × 10−5 and 1.6 × 10−5 resistant callus recovery frequencies and 59.7 and 37.9 cotransformation efficiencies respectively. No escapes were detected in dot blot analyses of 100 resistant calluses with a probe consisting of the bar coding region. Cotransformation efficiency, based on resistance to basta and β-glucuronidase staining of the leaf tissue of 115 regenerated plants, was 47%. Resistance tests and Southern analysis of seed progenies of three diploid transgenic plants demonstrated homozygous integration of multiple copies of the transgene at one locus at least in the first plant, heterozygous integration at one locus in the second plant and heterozygous integration at two loci in the third plant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00232225
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Plant regeneration from leaf and seed-derived calli and suspension cultures of the African perennial wild rice, Oryza longistaminata (1990)
    Springer
    Plant cell reports 9 (1990), S. 447-450 
    Details
    ISSN: 1432-203X
    Schlagwort(e): African wild rice ; Oryza longistaminata plant regeneration ; tissue culture ; cell suspension ; histology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Plant regeneration from 2-month-old callus cultures derived from immature leaves of 7-day-old aseptic seedlings and mature embryos of the African wild rice Oryza longistaminata was achieved at 20% and 100% frequency, respectively. The morphogenic potential of the embryo-derived calluses dropped from 100% at the third subculture to 12.5 % at the 12th subculture. Five-month-old morphogenic calluses were used to establish a fast-growing suspension culture which, when plated onto semisolid medium, still retained its ability to regenerate plantlets 9 months after initiation. Histological analyses demonstrated that late plant regeneration from established callus and suspension cultures occured through organogenesis, although some embryogenesis events may have taken place during initiation of these cultures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00232270
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Transformation of haploid, microspore-derived cell suspension protoplasts of rice (Oryza sativa L.) (1996)
    Springer
    Plant cell reports 15 (1996), S. 766-770 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Abbreviations: PEG = polyethylene glycol, T0 = regenerated transgenic plant, GUS = βglucuronidase, CaMV = cauliflower mosaic virus, ARE = anaerobic responsive element, OCS = octopine synthese, T1 = first generation progeny of transgenic plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We compared the transient activity of three cereal gene-derived promoter-gus fusions and the efficiency of selection mediated by three different selectable genes in a polyethylene glycol transformation system with haploid cell suspension protoplasts of rice. The maize ubiquitin promoter was found to be the most active in transformed protoplasts, and selection on ammonium glufosinate mediated by the bar gene was the most efficient for producing resistant calluses. Cotransformation of protoplasts with two separate plasmids carrying the gus and the bar genes, at either a 2:1 or 1:1 ratio, led to 0.8×10–5 and 1.6×10–5 resistant callus recovery frequencies and 59.7 and 37.9 cotransformation efficiencies respectively. No escapes were detected in dot blot analyses of 100 resistant calluses with a probe consisting of the bar coding region. Cotransformation efficiency, based on resistance to basta and β-glucuronidase staining of the leaf tissue of 115 regenerated plants, was 47%. Resistance tests and Southern analysis of seed progenies of three diploid transgenic plants demonstrated homozygous integration of multiple copies of the transgene at one locus at least in the first plant, heterozygous integration at one locus in the second plant and heterozygous integration at two loci in the third plant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050117
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Increased doubled haploid plant regeneration from rice (Oryza sativa L.) anthers cultured on colchicine-supplemented media (1994)
    Springer
    Plant cell reports 13 (1994), S. 432-436 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Rice ; Oryza sativa L ; chromosome doubling ; anther culture ; colchicine
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anther cultures. The addition of colchicine had no detrimental effects on the different anther culture efficiency parameters, but in some treatments led to significant enhancement of anther callusing frequency or callus green plant regenerating ability. The most efficient treatment raised doubled haploid plant recovery from 31% to 65.5%. These results suggest that post-plating colchicine treatment of anthers, since it was found to improve both anther culture efficiency and doubled haploid plant recovery frequency, could be integrated into rice doubled haploid plant production programmes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00231961
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Plant regeneration from haploid cell suspension-derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) (1992)
    Springer
    Plant cell reports 11 (1992), S. 618-622 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Mediterranean Rice ; Oryza sativa L. ; haploid protoplasts ; regeneration ; protoclonal variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract More than 750 plants were regenerated from protoplasts isolated from microspore callus-derived cell suspensions of the Mediterranean japonica rice Miara, using a nurse-feeder technique and N6-based culture medium. The mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. Flow cytometric evaluation of the DNA contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. Contrastingly, the DNA contents of leaf cell nuclei of the regenerated protoclones ranged from 1C to 5C including 60% 2C plants. This was consistent with the morphological type and the fertility of the mature plants. These results and the absence of chimeric plants suggest that polyploidization occurred during the early phase of protoplast culture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00236385
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Expression of a Bacillus thuringiensis cry1B synthetic gene protects Mediterranean rice against the striped stem borer (2000)
    Springer
    Plant cell reports 19 (2000), S. 1195-1202 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Bacillus thuringiensis ; cry1B ; Insect resistance ; Oryza sativa L. ; Synthetic gene ; Transgenic plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Bacillus thuringiensis Cry1Ba endotoxin, which was shown to exhibit a tenfold lower lethal concentration 50 (LC50) than Cry1Ac in a Striped Stem Borer (SSB) diet incorporation assay. The 1.950-bp synthetic cry1B gene, possessing an overall GC content of 58 %, was cloned under the control of the maize ubiquitin promoter first intron and first exon regions. The resulting vector, designated as pUbi-cry1B, was transferred to two commercial Mediterranean cultivars of rice, Ariete and Senia, using microprojectile acceleration-mediated transformation. Thirty-two and 47 T0 events were generated in cvs. Ariete and Senia, respectively. Southern blot and immunoblot analyses allowed the identification of 7 Senia and 1 Ariete events harbouring both an intact gene cassette and expressing Cry1B at a level ranging from 0.01% to 0.4% of the total soluble proteins. Three Senia and 1 Ariete events were found to be protected against second instar SSB larvae in whole plant feeding assays, exhibiting 90–100% mortality 7 days after infestation. Spatial and temporal variation in transgene expression was further examined in resistant event 64 of cv. Ariete. Stable accumulation of Cry1B, representing 0.4% of the total soluble proteins, was observed over the T2 to T4 generations in leaf tissue 20, 40, 70 and 90 days after germination in both young and old leaves and in internodes. Ariete event 64 was found to be fully protected from attacks of third and fourth instar SSB larvae over subsequent generations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990000247
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Gametic selection in anther culture of rice (Oryza sauva L.) (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 406-412 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Rice ; Japonica/indica ; Anther culture ; Isozyme markers segregation ; Linkage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The segregation and recombination of heterozygous isozyme markers have been monitored in anther culture derivatives (i.e., six nonmorphogenic microspore-derived callus [NMC] populations and two anther culture plant [ACP] populations) and F2 plants generated from six F1 hybrids of rice, including five japonica upland/improved indica tropical hybrids. The alleles in excess at some loci displaying skewed segregations in the F2s were consistently overrepresented in the NMC populations. These alleles were also generally found to be overabundant in the two ACP populations except for certain loci that contrastingly segregated in a 1∶1 ratio. Additional distortions were found to be specific to AC derivatives indicating the existence of in vitro gametic selection. Overall, however, the gametic selection in the ACP materials was neutral with regard to the indica and japonica differentiation. Estimates of linkages between markers borne by chromosome 6 using AC-derivative data were consistent with those noted in the F2s and with current knowledge of the isozyme locus linkage map. Given the average neutrality of gametic selection and the consistency of linkage relationships in the ACPs, their further use as rice molecular mapping and gene tagging populations can be investigated with confidence.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228684
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Molecular mapping of genes for resistance to rice blast (Pyricularia grisea Sacc.) (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 859-863 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Disease resistance ; Rice (Oryza sativa L.) ; Blast (Pyricularia grisea Sacc.) ; Restriction fragment length polymorphism (RFLP) ; Gene mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two dominant genes conferring complete resistance to specific isolates of the rice blast fungus, Pyricularia grisea Sacc., were located on the molecular map of rice in this study. Pi-l(t) is a blast resistance gene derived from the cultivar ‘LAC23’. Its map location was determined using a pair of nearly isogenic lines (NILs) and a B6F3 segregating population from which the isoline was derived. RFLP analysis showed that Pi-l(t) is located near the end of chromosome 11, linked to RZ536 at a distance of 14.0±4.5 centiMorgans (cM). A second gene, derived from the cultivar ‘Apura’, was mapped using a rice doubled-haploid (DH) population. This gene was located on chromosome 12, flanked by RG457 and RG869, at a distance of 13.5+-4.3 cM and 17.7+-4.5 cM, respectively. The newly mapped gene on chromosome 12 may be allelic or closely linked toPi-ta. (=Pi-4(t)), a gene derived from Tetep that was previously reported to be linked to RG869 at a distance of 15.4±4.7 cM. The usefulness of markers linked to blast resistance genes will be discussed in the context of breeding for durable blast resistance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224086
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