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  • 1
    Electronic Resource
    Electronic Resource
    Modification of competence for in vitro response to Fusarium oxysporum in tomato cells. II. Effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 89-96 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; Fusarium oxysporum f. sp. ; lycopersici ; Agrobacterium hormone synthetic genes ; Defense response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (Lycopersicon esculentum) cells to defend themselves against the fungal pathogen Fusarium oxysporum f. sp. lycopersici. Calluses from cvs ‘Davis’ and ‘Red River’, respectively resistant and susceptible to Fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from Agrobacterium tumefaciens, were used. The integration of Agrobacterium hormone-related genes into susceptible cv ‘Red River’ can bring the activation of defense processes to a stable competence as assessed by the inhibition of mycelial growth in dual culture and gem-tube elongation of Fusarium conidia, the determination of callose contents, peroxidase induction and ion leakage in the presence of fusaric acid. This is particularly true when the transformation results in a change of phytohormone equilibria towards an higher cytokin in concentration. On the contrary, in resistant cv ‘Davis’ the inhibition of both fungal growth in dual culture and conidia germination is higher when the hormone balance is modified in favour of the auxins. No significant effect was observed for ion leakage and peroxidase induction, probably because of a constitutive overproduction of cytokinins in ‘Davis’ cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222399
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  • 2
    Electronic Resource
    Electronic Resource
    Modification of competence for in vitro response to Fusarium oxysporum in tomato cells. I. Selection from a susceptible cultivar for high and low polysaccharide content (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 988-995 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; Fusarium oxysporum f.sp. lycopersici ; Polysaccharides ; Active defence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant cell walls play a major role in the outcome of host-parasite interactions. Wall fragments released from the plant, and/or the fungal pathogen, can act respectively as endogenous and exogenous elicitors of the defence response, and other wall components, such as callose, lignin, or hydroxyproline-rich glycoproteins, can inhibit pathogen penetration and/or spreading. We have previously demonstrated that calli from tomato cultivars resistant in vivo to Fusarium oxysporum f.sp. lycopersici show a high amount of polysaccharides in vitro. The aim of the present work was to assess the possible role of polysaccharide content and/or synthetic capacity in determining the competence of plant cells for active defence. For this purpose, tomato cell clones with increased and decreased polysaccharide (FL+, FL-) and callose (A+, A-) content have been selected by means of specific stains as visual markers and tested for the effect of these changes on the extent of response to Fusarium. The analysis of several parameters known to be indicative of active defence (cell browning after elicitor treatment, peroxidase and β-glucanase induction and inhibition of fungal growth in dual culture) clearly shows that FL+ and A+ clones have acquired an increased competence for the activation of defence response. The results are thoroughly discussed in terms of an evaluation of the relative importance of constitutive and/or inducible polysaccharide synthetic capacity for plant response to pathogens, and their possible regulation by plant physiological backgrounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225793
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    Paper (German National Licenses)
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