ISSN:
1365-3040
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract. PEP-carboxylase was extracted and partially purified from nine species of the genus Sedum and three species of the genus Kalanchoe, all performing CAM. Immunological and molecular properties of these enzymes were compared. Molecular weight estimation with gradient slab gels showed identical molecular weights of about 232,000 for all PEP-carboxylases. Ouchlerlony double-diffusion analysis, immunotitralion and SDS polyacrylamide clectrophoresis indicated the presence of PEP-c dimers consisting of monomers of MW 105,000 and 115,000. A model of PEP-c substructure is proposed. The results are discussed in the context of CAM performance in the genus Sedum
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/1365-3040.ep11572356
Permalink