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  • 1
    ISSN: 1573-5117
    Keywords: Great Lakes ; Herring Gull ; diet ; winter ; spring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the Great Lakes, the Herring Gull (Larus argentatus) is a prominent member of the aquatic bird community, and has been used to monitor spatial and temporal trends in contaminant levels. To understand more fully contaminant loading outside the breeding season, we analysed the contents of 1298 freshly regurgitated pellets and 179 fresh faeces, collected in March and early April 1978–83, and between late December and late February 1990–91, from the vicinity of breeding colonies in Lakes Ontario and Erie, the Niagara River, Detroit River, and south-eastern parts of Lake Huron. Most adult Herring Gulls from the Great Lakes population winter in these areas, but there is no published account of their food habits other than during the breeding season. Most pellets from colonies close to large urban centres contained remains of garbage, as well as various fish species. Small mammals, notably Deer Mice (Peromyscus maniculatus) dominated the early spring diet at Lake Huron colonies near agricultural areas. At all other sites fish predominated in pellets and faeces, but garbage items were also recorded regularly. The species of fish consumed varied regionally, probably reflecting local availability. In Lake Ontario, Rainbow Smelt (Osmerus mordax) and Alewife (Alosa pseudoharengus) occurred most frequently in samples, whereas Freshwater Drum (Aplodinotus grunniens) was the main fish prey in Lake Erie and the Detroit River. Dietary differences were apparent between years, within seasons, and amongst areas. While these may have reflected some real differences in food availability, interpretation of these results was confounded by various biases inherent in the sampling of pellets and faeces to determine diet in such an opportunistic species. Therefore, it would be unwise to draw rigid conclusions as to regional or seasonal differences in the diets of piscivorous birds, based upon analyses of diet from only a small sample of sites or years. Herring Gulls appear to feed mainly on fish and garbage in winter and early spring on the lower Great Lakes (much as during the breeding season), but any locally abundant food source is probably exploited opportunistically.
    Type of Medium: Electronic Resource
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  • 2
  • 3
    Publication Date: 2015-12-02
    Description: Restriction-modification (R-M) systems pose a major barrier to DNA transformation and genetic engineering of bacterial species. Systematic identification of DNA methylation in R-M systems, including N 6 -methyladenine (6mA), 5-methylcytosine (5mC) and N 4 -methylcytosine (4mC), will enable strategies to make these species genetically tractable. Although single-molecule, real time (SMRT) sequencing technology is capable of detecting 4mC directly for any bacterial species regardless of whether an assembled genome exists or not, it is not as scalable to profiling hundreds to thousands of samples compared with the commonly used next-generation sequencing technologies. Here, we present 4mC-Tet-assisted bisulfite-sequencing (4mC-TAB-seq), a next-generation sequencing method that rapidly and cost efficiently reveals the genome-wide locations of 4mC for bacterial species with an available assembled reference genome. In 4mC-TAB-seq, both cytosines and 5mCs are read out as thymines, whereas only 4mCs are read out as cytosines, revealing their specific positions throughout the genome. We applied 4mC-TAB-seq to study the methylation of a member of the hyperthermophilc genus, Caldicellulosiruptor , in which 4mC-related restriction is a major barrier to DNA transformation from other species. In combination with MethylC-seq, both 4mC- and 5mC-containing motifs are identified which can assist in rapid and efficient genetic engineering of these bacteria in the future.
    Keywords: Nucleic acid modification, Phsyical and Biochemical Characterisation of DNA
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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  • 4
    Publication Date: 1994-04-01
    Print ISSN: 0018-8158
    Electronic ISSN: 1573-5117
    Topics: Biology
    Published by Springer
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  • 5
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    In:  XXVIII General Assembly of the International Union of Geodesy and Geophysics (IUGG)
    Publication Date: 2023-08-09
    Description: Aotearoa New Zealand's GeoNet programme was launched with an open data framework to support research, monitoring, and geohazard response at its core. Open data has proven to be hugely successful for GeoNet funders and user community and continues to be at the heart of GeoNet. GeoNet is now evolving to develop new services, storage, processing, and dissemination mechanisms to meet emerging data usage requirements (for example: requests to access large data volumes). Over the last few years, GeoNet has taken some major steps forward in supporting data access, usability, and resilience in data operations: GeoNet moved its diverse data collection, processing and storage to the cloud (Amazon – Web Services). This includes the development of a data lake strategy; GeoNet released TILDE, a system to disseminate low to medium sample rate datasets in a common format, that supports access via an Application Programming and a Graphical User Interfaces; GeoNet partnered with AWS Open Data Sponsorship Programme to make a copy of its data freely available for large data query access. GeoNet continues to face other challenges: understanding and tracking impact and data use (user registration and authentication for data access?), quality monitoring, adoption and impact of new initiatives (e.g. citizen science), improving integration of Te Ao Māori, and significant expansion of data volumes and variety. But with challenges come opportunities and GeoNet looks forward to working with the broader international community and fostering discussions to how best to balance data funders/providers/users needs whilst keeping open data as a core principle.
    Language: English
    Type: info:eu-repo/semantics/conferenceObject
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