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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 13 (1990), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 60 (2002), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Five reproductive classes of cobia Rachycentron canadum, caught along the Gulf of Mexico and the south-east Atlantic coast of the U.S.A., are described during the annual reproductive cycle. These are based upon changes in the testicular germinal epithelium and the stages of germ cells that are present: early maturation, mid maturation, late maturation, regression and regressed. During early maturation, the germinal epithelium is continuous from the testicular ducts to the periphery of the testis and active spermatogenesis occurs throughout the testis. In mid maturation, the germinal epithelium near the ducts becomes discontinuous, but it remains continuous distally. In late maturation, a discontinuous germinal epithelium extends all along the lobules to the testicular periphery; lobules are swollen with sperm and there is minimal spermatogenesis. The regression class is characterized by a discontinuous epithelium throughout the testis, sperm storage and widely scattered spermatocysts. Spermatogonial proliferation also occurs along the lobule walls and at the periphery of the testis. In regressed testes, spermatogonia exist only in a continuous or discontinuous germinal epithelium, although residual sperm are nearly always present in the lobules and ducts. The presence or absence of sperm is not an accurate indicator of reproductive classes. At the periphery of the testis in the regression and regressed classes, the distal portions of lobules elongate as cords of cells containing spermatogonia and Sertoli cells. All reproductive classes can be identified in paraffin sections, although plastic sections provide better resolution. Using maturation classes defined by changes in the germinal epithelium to describe testicular development and spermatogenesis gives a more accurate picture than does using the traditional terminology.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 53 (1998), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During the annual reproductive cycle, the lobular testis of Centropomus undecimalis undergoes height, width, and morphological changes which reflect five reproductive classes: regressed; early, mid-, and late maturation; and regression. Histological criteria, particularly differences between continuous and discontinuous germinal epithelia, are used to distinguish these five classes, even though they are only reference points within the annual gonadal cycle. A mechanism for lobular growth during early, mid-, and late maturation is presented; and it is hypothesized that a permanent germinal epithelium first appeared in the fishes. Throughout the year, periodic acid-Schiff-positive macro-melanophage centres and PAS-positive granulocytes are observed in the testis. They are most abundant after the breeding season, and they may be involved in focal tissue degradation.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 35 (2004), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sperm were collected in Florida from wild common snook, Centropomus undecimalis (Bloch), and were shipped to Louisiana State University for analysis and cryopreservation. Threshold activation of sperm (10% motility) occurred at 370 mOsmol kg−1, and complete activation occurred at 680 mOsmol kg−1. These values were significantly different. Sperm samples stored at 1°C in Hanks' balanced salt solution (HBSS) or in 0.6% NaCl solution at 200 mOsmol kg−1 retained motility for as long as 22 days. Mean motility remained above 50% for 9 days for sperm stored in HBSS and for 7 days for sperm stored in NaCl solution. Sperm exposed to 5% dimethyl acetamide (62±10%; mean±SD), 10% dimethyl sulphoxide (DMSO) (39±16%), 5% glycerol (26±5%) or 10% glycerol (6±2%) for 30 min had significantly lower motility than did unexposed sperm (89±9%). When used as a cryoprotectant, samples frozen with 5% or 10% DMSO or 5% methanol had significantly higher post-thaw motility than did samples frozen with other cryoprotectants. Sperm cryopreserved with 10% DMSO (38±12%) had significantly higher post-thaw motility than did sperm cryopreserved with 15% DMSO (19±10%) or 20% DMSO (4±4%). There were no significant differences in hatch rates of eggs fertilized with fresh sperm (54±29%) or cryopreserved sperm (41±35%). Survival to first feeding was not different between fish produced with fresh sperm (37±30%; range, 0–86%) or with thawed sperm (24±29%; 0–77%). Transport of sperm to a cryopreservation laboratory and back to a hatchery for thawing and use enabled collaboration between groups with specific expertise and provides a model for the application of cryopreservation by transport of fresh and frozen samples.
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  • 5
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 53 (1998), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Common snook Centropomus undecimaliswere sampled monthly from the Jupiter–Lake Worth area of Florida's Atlantic coast during 1989 and 1991 (1452 fish) and from Tampa Bay on Florida's Gulf of Mexico coast during 1988 and 1989 (2090 fish). Group-synchronous oocyte development was demonstrated. Ovarian maturation began during March or April on both coasts. Spawning was first detected histologically in April during 1989 and 1991 on the Atlantic coast and during May in 1988 and in April in 1989 on the Gulf coast. In each year, spawning ended during October on the Atlantic coast and during September on the Gulf coast. Ovarian histological evidence suggested that individual females may spawn every 1·1–2·5 days between 1400 and about 2000 hours. Final oocyte maturation occurred independently of either tidal cycle or lunar phase, and some common snook were observed in prespawning or spawning condition on every day sampled. Spawning occurred in or near major inlets to the Atlantic Ocean and the Gulf of Mexico, in secondary passes to larger inland bays and bayous, and around nearshore islands.
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  • 7
    ISSN: 1573-5117
    Keywords: Spermatozoa ; ultrastructure ; fertilization ; phylogeny ; Zenarchopteridae ; Poeciliidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a cladistic analysis, poeciliids and zenarchopterids homoplasically show elongation and flattening of the nucleus at right angles to the plane of the central axonemal singlets; in both the tip of the nucleus appears rounded in the plane of flattening but pointed in the plane at right angles. The two families differ in the distribution of mitochondria in the .elongate midpiece: circumferential in poeciliids but bilateral in zenarchopterids. In poeciliid sperm and independently in Zenarchopterus, the individual mitochondria are considerably more extensive circumferentially than longitudinally; they differ in poeciliids in being C-shaped. In Hemirhamphodon they are moderately elongate. In Dermogenys and Nomorhamphus they have been modified monophyletically as a pair of elongate mitochondrial derivatives. A wide cytoplasmic periaxonemal sheath (not seen in poeciliids) appears to have developed monophyletically in the ancestry of Hemirhamphodon, Dermogenys and Nomorhamphus with acquisition of radial rodlets only in Hemirhamphodon. A distinctive development in poeciliids is the submitochondrial net. Poeciliids have greatly reduced the axonemal fins which are a synapomorphy of the Actinopteri. Exocoetoids have retained well developed fins in Arrhamphus, Dermogenys and Nomorhamphus but reduction has occurred in Zenarchopterus, in which the fins are small, and, apparently independently, in Hemirhamphodon in which fins are absent. A posterior extension of the nucleus over the base of the axoneme is C-shaped and embraces almost the entire circumference of the axoneme in poeciliids but, independently developed, in zenarchopterids is a ‘dorsal’ plate. Its absence in Hemirhamphodon is computed as a loss. These modifications relative to the aquasperm condition are deduced to have been occasioned by the adoption of internal fertilization. To what extent they are constrained by features of the genome peculiar to poeciliids, zenarchopterids or atherinomorphs or are demanded by minute differences in fertilization biology, or by a combination of the two, is not at present determinable.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 156 (1978), S. 419-437 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Testis structure in four species of goodeid teleosts is described. Testicular tubules terminate blindly at the testis periphery where spermatogonia are located. In goodeid teleosts, development of sperm takes place synchronously within cysts whose periphery is made up of a single layer of Sertoli cells. Upon completion of spermiogenesis, spermiation ensues wherein sperm are shed, as spermatozeugmata, into the testis efferent duct system. Subsequently, Sertoli cells, which comprised the cyst periphery, transform into efferent duct cells.Sertoli cells phagocytize residual bodies and are involved in the formation of spermatozeugmata. The structure of the goodeid spermatozeugmatum is quite different from that observed in the related poeciliids. It is concluded, in view of this and other considerations, that the goodeids and poeciliids have independently evolved solutions to the problems of internal fertilization and gestation.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 146 (1975), S. 229-249 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The structure of the testis of Poecilia latipinna is described with particular reference to Sertoli cell-germ cell relationships during development and maturation of the germinal cyst. The cyst develops when primary spermatocytes become surrounded by a single layer of Sertoli cells at the testis periphery. As spermatogenesis and then spermiogenesis proceed, the cyst moves centrally in the testis toward the ducts comprising the vasa efferentia. In addition to being a structural part of the germinal cyst, the Sertoli cells phagocytize residual bodies cast off by developing spermatids and form an association with mature sperm, which resembles that observed in mammals, before the sperm are released into the vasa efferentia as a spermatozeugmata.The results of this investigation are discussed in view of what is known concerning testis structure in other teleosts and similarities between cell functions in teleosts and mammals. It is concluded that teleost Sertoli cells, teleost lobule boundary cells and mammalian Sertoli cells are homologous.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 165 (1975), S. 89-102 
    ISSN: 1432-0878
    Keywords: Spermiogenesis (teleost) ; Microtubules ; Centriolar complex ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary During nuclear elongation in spermatids of Gambusia affinis, a deep fossa is formed at the base of the nucleus in which the centriolar complex and proximal portion of the flagellum reside. To stabilize the positional relationship between the nucleus and centriolar complex, while nuclear morphogenesis is taking place, a series of microtubules develop which emanate from the centriolar complex and extend to the nuclear envelope lining the fossa. Buttressing microtubules also develop within the nuclear fossa which both originate and insert along the nuclear envelope. These appear to stabilize nuclear shape prior to the time when chromatin condensation has proceeded to the stage where it could lend structural stability to nuclear form. Microtubules develop only after specific nuclear morphogenic events have taken place. It is therefore concluded that the spermatid nucleus is capable of “self-assembly” involving microtubules in a supportive role in addition to stabilizing the nuclear-flagellar relationship in G. affinis. The pattern of nuclear fossa-associated microtubules in G. affinis is significantly different from that observed in other poeciliid teleosts indicating a degree of species specificity with regard to both the timing of appearance and total number of microtubules.
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