ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Debrisoquine
;
Urine
;
Phenotype
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary An isocratic HPLC method is described. It allows the simultaneous determination of debrisoquine and 4-hydroxydebrisoquine in urine. Sample preparation involves a rapid and simple (one step) liquid-liquid extraction without derivatization. Separation, followed by UV-detection, is carried out on a nitrile column in reversephase mode with a binary, aqueous mobile phase. This rapid assay uses a new internal standard (guabenxane) and allows the determination of urinary concentrations with good precision and appropriate sensitivity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02327897
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