ISSN:
0749-503X
Keywords:
DNA sequence
;
ras related
;
membrane localization
;
palmitoylation
;
C-terminal modification
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The ras protein represent a unique example of membrane proteins which apparently do not utilize the secretory pathway for their membrane localization. Instead, it is belived that palmaitic acid, covalently attached to the protein, acts as an anchor to the membranes. Recent identification of yeast mutants defective in the processing of the ras proteins has provideda novel approach for defining these biosynthetic process. We report here the charcterization of yeast DPR1, a gene essential for the processing of the ras proteins. The sequence of the gene indicates that it encodes a protein of 431 amino acids which contains no significant homology with any known proteins. It is a relatively hydrophilic protein of cysteine. The DPR1 gene product product has been identified in a cell-free translation system as a proteinhaving an apparent molecular weight of 43 hd. This represents the first step in the translation system as a protein having an apparent molecular weight of 43 kd. This represents the first step in the investigation of a novel protein-processing pathway, one that id distinct from the secretory pathway.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/yea.320040405
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