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  • 1
    Electronic Resource
    Electronic Resource
    Coprological study of theDicrocoelium dendriticum (Digenea) egg elimination by cattle in highland areas in León Province, Northwest Spain (1993)
    Springer
    Parasitology research 79 (1993), S. 488-491 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Dicrocoelium dendriticum egg output in cattle from five locations of the Porma river basin (León Province, Northwest Spain) was studied at monthly intervals between March 1986 and March 1987. We foundD. dendriticum eggs in 37.64% of the 1251 samples examined, and the number of eggs per gram (epg) ranged from 10 to 1000 (average, 41.65±2.73). The main eggelimination period, for prevalence, was autumn-winter. The average epg values were quite similar during all months except March, when the maximum was detected. The infection prevalence increased in cattle aged up to 2 years and then a slight, gradual decrease was observed with increasing host age. The average epg values ranged from 40.08±3.24 to 48.33±12.42 in all age groups except the oldest animals, for which the figures were lower. The rate of egg elimination was higher in animals that came from locations situated at a greater altitude.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931589
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  • 2
    Electronic Resource
    Electronic Resource
    Diplectanidae (Monogenea) infestations of sea bass,Dicentrarchus labrax (L.), from the Spanish Mediterranean area (1991)
    Springer
    Parasitology research 77 (1991), S. 307-314 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In the present study,Diplectanum laubieri was redescribed from material obtained from wild and cultured fish. The population dynamics ofD. aequans andD. laubieri under culture conditions was studied. The prevalence and mean intensity (average number of worms per parasitized fish) were 80.64% and 112 forD. aequans and 67.74% and 59.61 forD. laubieri, respectively. Both Diplectanidae exhibited seasonal variations, with maximal infection levels occurring in winter, suggesting continuous parasites recruitment, and infection levels tended to increase with host age. Male hosts were more heavily infested than females. The apparent positive association betweenD. aequans andD. laubieri was not confirmed statistically. The Diplectanidae showed definite and different patterns of gill distribution. Histopathological gill damage mainly consisted of hyperplasia of the epithelium, fusion of branchial lamellae and the presence of hemorrhagic and inflammatory foci with leucocytic infiltration. The results demonstrated the pathological importance of both Diplectanidae for the cultivation of sea bass.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00930906
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  • 3
    Electronic Resource
    Electronic Resource
    IgG antibody response to ES or somatic antigens of Dicrocoelium dendriticum (Trematoda) in experimentally infected sheep (2000)
    Springer
    Parasitology research 86 (2000), S. 472-479 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ELISA technique was used to study the kinetics of IgG antibodies against excretory–secretory (ES) and somatic (So) antigens of Dicrocoelium dendriticum in the sera of 32 lambs: 12 experimentally infected with 1,000 metacercariae, 12 with 3,000 and 8 controls. Both antigen types were used at a 2 μg/ml concentration. Dilutions of sera and the anti-sheep IgG peroxidase conjugate were: 1:200 and 1:6,000, respectively. Optical density values for each type of antigen in the two infected groups were higher than the cut-off point from day 30 post infection (p.i.), showing positive infection. Maximum antibody levels were observed 60 days p.i. and remained high until the experiment ended 180 days p.i. This pattern was similar for both ES and So antigens, although with slightly lower figures in the latter. Antibody kinetics were very similar for each of the two doses, except on day 30 p.i. No correlation between the antibody level and parasite burden could be established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050696
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