Publication Date:
2015-05-08
Description:
Miscanthus is a perennial C 4 grass that has recently become an important bioenergy crop. The efficiency of breeding improved Miscanthus biomass cultivars could be greatly increased by marker-assisted selection. Thus, a high density genetic map is critical to Miscanthus improvement. In this study, a mapping population of 261 F 1 progeny was developed from a cross between two diploid M. sinensis cultivars, ‘Strictus’ and ‘Kaskasde’. High density genetic maps for the two parents were produced with 3,044 newly developed single nucleotide polymorphisms (SNPs) obtained from restriction-site associated DNA sequencing, and 138 previously mapped GoldenGate SNPs. The female parent (‘Strictus’) map spanned 1,599 cM, with 1,989 SNPs on 19 linkage groups, and an average inter-marker spacing of 0.8 cM. The length of the male parent (‘Kaskade’) map was 1,612 cM, with 1,821 SNPs, and an average inter-marker spacing of 0.9 cM. The utility of the map was confirmed by locating quantitative trait loci (QTL) for the zebra stripe trait, which was segregating in this population. Three QTL for zebra stripe presence/absence ( zb1 , zb2 on LG 7, and zb3 on LG 10) and three for zebra stripe intensity ( zbi1 , zbi2 , zbi3 on LGs 7, 10, 3) were identified. Each allele that caused striping was recessive. Incomplete penetrance was observed for each zb QTL, but penetrance was greatest when two or more zb QTL were homozygous for the causative alleles. Similarly, the intensity of striping was greatest when two or more zbi QTL were homozygous for alleles that conferred the trait. Comparative mapping indicated putative correspondence between zb3 and/or zbi2 on LG 10 to previously sequenced genes conferring zebra stripe in maize and rice. These results demonstrate that the new map is useful for identifying marker-trait associations. The mapped markers will become a valuable community resource, facilitating comparisons among studies and the breeding of Miscanthus . This article is protected by copyright. All rights reserved.
Print ISSN:
1757-1693
Electronic ISSN:
1757-1707
Topics:
Energy, Environment Protection, Nuclear Power Engineering
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