Publication Date:
2019
Description:
〈sec〉〈st〉Synopsis〈/st〉〈p〉〈textbox textbox-type="graphic"〉〈p〉〈inline-fig〉〈/inline-fig〉〈/p〉〈/textbox〉〈/p〉
〈p〉Centriolar satellites are implicated in cellular functions such as centriole duplication, centrosome maturation and ciliogenesis, but their precise composition and assembly properties are still poorly defined. Spatial and proteomic profiling now reveals their interactome and centrosome-independent features.〈/p〉
〈p〉 〈l type="unord"〉〈li〉〈p〉BioID of 22 centriolar satellite proteins identifies 2113 proximity interactions amongst 660 proteins.〈/p〉〈/li〉
〈li〉〈p〉Six additional centriolar satellite proteins were identified.〈/p〉〈/li〉
〈li〉〈p〉There are multiple microscopically-resolvable satellite subpopulations.〈/p〉〈/li〉
〈li〉〈p〉Satellite composition is largely unaffected by centriole depletion or disruption of the microtubule cytoskeleton.〈/p〉〈/li〉
〈li〉〈p〉PCM1 depletion results in a dramatic change in the proximity interaction landscape of centriolar satellite components.〈/p〉〈/li〉〈/l〉 〈/p〉〈/sec〉
Print ISSN:
0261-4189
Electronic ISSN:
1460-2075
Topics:
Biology
,
Medicine
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