ISSN:
0006-3592
Keywords:
enzyme thermistor
;
immobilized invertase
;
kinetic properties
;
screening of Con A conjugates
;
biospecific adsorption
;
bead cellulose
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Screening and design of immobilized biocatalysts (IMBs) is a time-consuming process. An ideal process should be universal, fast, convenient, precise, and reproducible. Many of these requirements are met by enzymic flow microcalorimeters, also known as enzyme thermistors (ETs) or thermal assay probes (TAPs). Adaptation of ETs to real measurements of reaction rates requires coupling of the mathematical description of the reaction-diffusion phenomena in the ET column with heat balance and, subsequently, experimental verification of the mathematical model. This article presents such a process developed as an adaptation of ETs for the characterization of the microkinetic properties of IMBs and their further application for screening of IMBs. The IMBs characterized were the preparations of invertase, biospecificaly adsorbed on concanavalin A conjugated to activated bead cellulose. © 1994 John Wiley & Sons, Inc.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260430404
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