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  • 1
    Electronic Resource
    Electronic Resource
    Structure of eukaryotic 5S ribonucleic acid: a study of Saccharomyces cerevisiae 5S RNA with ribonucleases (1982)
    s.l. : American Chemical Society
    Biochemistry 21 (1982), S. 4823-4830 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00262a047
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  • 2
    Electronic Resource
    Electronic Resource
    Binding sites of ribosomal proteins on prokaryotic 5S RNAs: a study with ribonucleases (1982)
    s.l. : American Chemical Society
    Biochemistry 21 (1982), S. 2313-2320 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00539a007
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  • 3
    Electronic Resource
    Electronic Resource
    Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases (1981)
    s.l. : American Chemical Society
    Biochemistry 20 (1981), S. 7301-7307 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00528a039
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  • 4
    Electronic Resource
    Electronic Resource
    Comparison of eubacterial and eukaryotic 5S RNA structures: a chemical modification study (1985)
    s.l. : American Chemical Society
    Biochemistry 24 (1985), S. 241-250 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00323a002
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  • 5
    Electronic Resource
    Electronic Resource
    Alternative conformers of 5S ribosomal RNA and their biological relevance (1985)
    s.l. : American Chemical Society
    Biochemistry 24 (1985), S. 2284-2291 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00330a024
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  • 6
    Electronic Resource
    Electronic Resource
    Principles of Siphons (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 22 (1991), S. 0 
    Details
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A simple siphon raises water over a crest and discharges it at a lower level. As water flows through a siphon, energy due to pressure and elevation is either lost to pipe friction or converted to velocity energy. For most practical siphons, between 50 and 75% of the elevation energy available to drive flow will be converted to velocity energy.A standpipe covered with a dome will begin to siphon when the standpipe flows full. During siphoning, the flow rate may be several times higher than the flow through an uncovered standpipe of the same size. Furthermore, the covered standpipe can be designed to lower the water level to a point below the top of the standpipe before siphoning is allowed to stop.Flow into the covered standpipe can be drawn from the bottom of the reservoir by extending the skirt of the dome. A vent tube allows easy adjustment of the elevation at which air enters the dome and siphoning stops. Flow rate and water level required to start the siphon depend on the pipe diameter. Some flow through the standpipe will occur before siphoning begins. Flow into the reservoir must be greater than the flow required to start siphoning. Maximum flow through the standpipe during siphoning depends on pipe diameter and the elevation energy driving the flow.Adding a trap to the outlet of the covered standpipe eliminates the leakage flow prior to the start of siphoning. When air bubbles out the trap at the end of the standpipe, pressure inside the dome is released suddenly allowing the water level to jump above the critical level needed to start siphoning. For reliable operation, the crest height of the trap should be about equal to one diameter of the standpipe. The vent tube opening must be below the top of the standpipe to prevent premature overflow of the standpipe. Because a trapped outlet siphon has no minimum flow limit, the standpipe can be sized to carry any maximum flow.While the trapped outlet siphon eliminates low rate leakage flow prior to the onset of siphoning, the height differential between the start and stop of siphoning cannot be as small or as easily adjusted as with a covered standpipe without a trap. The simple siphon is convenient for some applications, but it does not lend itself to automatic cycling operation. The choice depends on the needs of the situation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-7345.1991.tb00710.x
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  • 7
    Electronic Resource
    Electronic Resource
    General vectors for archaeal hyperthermophiles: Strategies based on a mobile intron and a plasmid (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 18 (1996), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Although there are currently no cloning and expression vectors available for archaeal hyperthermophiles, small cryptic plasmids have been characterized for these organisms as well as viruses and introns capable of spreading between cells. Below, we review the recent progress in adapting these genetic elements as vectors for Pyrococcus furiosus and Sulfolobus acidocaldarius. An efficient and reliable transformation procedure is described for both organisms. The potential of the mobile intron from Desulfurococcus mobilis, inserted into the bacterial vector pUC18 to generate a new type of vector, was investigated in S. acidocaldarius. A polylinker was inserted upstream from the open reading frame encoding the homing enzyme I-DmoI. Both the polylinker and a 276 bp fragment of the tetracycline gene from pBR322 could be inserted into the intron-plasmid construct and spreading still occurred in the culture of S. acidocaldarius. Experiments are in progress to test the co-mobility of the alcohol dehydrogenase and β-galactosidase genes from Sulfolobus species with the intron. A shuttle vector pCSV1 was also produced by fusing the pGT5 plasmid from Pyrococcus abyssi and the bacterial vector pUC19 which, on transformation, is stable in both organisms without selection. Growth inhibition studies indicate that both P. furiosus and S. acidocaldarius are sensitive to the antibiotics carbomycin, celesticetin, chloramphenicol and thiostrepton as well as butanol and butylic alcohol. Spontaneous mutants resistant to these drugs have been isolated carrying single site mutations in their 23S rRNA gene; they include mutants of S. acidocaldarius resistant to chloramphenicol, carbomycin and celesticetin with the mutation C2452U and thiostrepton-resistant mutants of P. furiosus carrying the mutation A1067G (both numbers corresponding to Escherichia coli 23S rRNA). These mutated genes are being developed as selective markers. Moreover, two β-galactosidase genes from P. furiosus have been cloned as possible phenotypic markers; one of these exhibits maximum activity at 95°C with O-nitrophenyl β-d-galactopyranoside as substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1996.tb00229.x
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  • 8
    Electronic Resource
    Electronic Resource
    Mobile elements in archaeal genomes (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 206 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The recent availability of several archaeal genome sequences has provided a basis for detailed analyses of the frequency, location and phylogeny of archaeal mobile elements. All the known elements fall into two main types, autonomous insertion sequence (IS) elements and the non-autonomous miniature inverted repeat element (MITE)-like elements. Both classes are considered to be mobilized via transposases that are encoded by the IS elements, although mobility has only been demonstrated experimentally for a few elements. The number, and diversity, of the elements differs greatly between the genomes. At one extreme Sulfolobus solfataricus P2 and Halobacterium NRC-1 are very rich in elements while Methanobacterium thermoautotrophicum contains none. The former also show examples of complex clusters of interwoven elements. An analysis of the genomic distribution in S. solfataricus suggests that the putative oriC and terC regions act as barriers for the mobility of both IS and MITE-like elements. Moreover, the very high level of truncated IS elements in the genomes of S. solfataricus, Sulfolobus tokodaii and Thermoplasma volcanium suggests that there may be a cellular mechanism for selectively inactivating IS elements at a point when they become too numerous and disadvantageous for the cell. Phylogenetically, archaeal IS elements are confined to 11 of the 17 known families of bacterial and eukaryal IS elements where some generate distinct subgroups. Finally, DNA viruses, plasmids and DNA fragments can also be inserted into, and excised from, archaeal genomes by means of an integrase-mediated mechanism that has special archaeal characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb10999.x
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  • 9
    Electronic Resource
    Electronic Resource
    The genetic element pSSVx of the extremely thermophilic crenarchaeon Sulfolobus is a hybrid between a plasmid and a virus (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 34 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A new Sulfolobus islandicus strain, REY15/4, harboured both a novel fusellovirus, SSV2, and a small plasmid, pSSVx. The plasmid spread in S. solfataricus P1 together with the virus after infection with either the supernatant of a culture of REY15/4 or purified virus. Spreading of the plasmid required co-transfection with either SSV2 or the related SSV1 as helpers. Virus purified from REY15/4 constituted a mixture of two sizes of particles, one with the dimensions of a normal fusellovirus and the other smaller. Cloned SSV2 produced only the larger particles and only SSV2 DNA, indicating that the smaller particles contained pSSVx packaged into capsids made up of SSV2 components. The 5.7 kb genome of pSSVx revealed regions of high sequence similarity to the cryptic Sulfolobales plasmids pRN1, pRN2 and pDL10. Thus, pSSVx belongs to the family of pRN plasmids that share a highly conserved region, which probably constitutes the minimal replicon. They also contain a variable region showing no sequence similarity. In pSSVx, this region contains three open reading frames (ORFs), two of which are juxtapositioned and show high sequence similarity to a tandem of ORFs in fusellovirus genomes. Neither pRN1 nor pRN2, which lack this tandem, spread in the presence of the fuselloviruses, which implies that the sequences of these ORFs enable pSSVx to use the packaging system of the viral helpers for spreading.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01573.x
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  • 10
    Electronic Resource
    Electronic Resource
    Identification of novel non-coding RNAs as potential antisense regulators in the archaeon Sulfolobus solfataricus (2005)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 55 (2005), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: By generating a specialized cDNA library from the archaeon Sulfolobus solfataricus, we have identified 57 novel small non-coding RNA (ncRNA) candidates and confirmed their expression by Northern blot analysis. The majority was found to belong to one of two classes, either antisense or antisense-box RNAs, where the latter only exhibit partial complementarity to RNA targets. The most prominent group of antisense RNAs is transcribed in the opposite orientation to the transposase genes, encoded by insertion elements (transposons). Thus, these antisense RNAs may regulate transposition of insertion elements by inhibiting expression of the transposase mRNA. Surprisingly, the class of antisense RNAs also contained RNAs complementary to tRNAs or sRNAs (small-nucleolar-like RNAs). For the antisense-box ncRNAs, the majority could be assigned to the class of C/D sRNAs, which specify 2′-O-methylation sites on rRNAs or tRNAs. Five C/D sRNAs of this group are predicted to target methylation at six sites in 13 different tRNAs, thus pointing to the widespread role of these sRNA species in tRNA modification in Archaea. Another group of antisense-box RNAs, lacking typical C/D sRNA motifs, was predicted to target the 3′-untranslated regions of certain mRNAs. Furthermore, one of the ncRNAs that does not show antisense elements is transcribed from a repeat unit of a cluster of small regularly spaced repeats in S. solfataricus which is potentially involved in replicon partitioning. In conclusion, this is the first report of stably expressed antisense RNAs in an archaeal species and it raises the prospect that antisense-based mechanisms are also used widely in Archaea to regulate gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2004.04428.x
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