ISSN:
1433-4909
Keywords:
Key words Moderately halophilic bacteria
;
Halomonas
;
Chromohalobacter
;
Genome analysis
;
Pulsed-field gel electrophoresis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The genomes of 11 moderately halophilic bacteria belonging to the genera Halomonas and Chromohalobacter have been analyzed by restriction endonuclease digestion and pulsed-field gel electrophoresis (PFGE). By using the infrequently cutting restriction endonucleases SpeI and SwaI, highly characteristic fingerprintings were obtained for each of the isolates studied. On the basis of the lengths of the SpeI and SwaI fragments, separated by PFGE, the genome size of the 11 strains studied was estimated. The genome size for 8 Halomonas strains ranged from 1450 to 2830 kb, whereas for the 3 Chromohalobacter strains studied it ranged from 1770 to 2295 kb. Finally, we show that macrorestriction fingerprints could be a useful tool to elucidate the taxonomic position of bacteria belonging to the Halomonas–Deleya complex.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007920050089
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