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  • 1
    Electronic Resource
    Electronic Resource
    The Plastid System of Normal and Bleached Euglena gracilis (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The plastid system of “normal”Euglena gracilis strain Z was found to be extremely unstable. Under normal culture conditions in the light about 1 or 2% of the cells were found to have lost spontaneously the capacity to green on further culturing. Cells treated with streptomycin, heat or U.V. all lost the capacity to green on further culturing. Bleached cells whether appearing spontaneously or by induction with streptomycin, heat, or U.V. light, were all found to possess within them organelles which were identified as proplastids.The proplastids of some “bleached” strains were capable of synthesizing porphyrins when grown in a standard culture medium. Others synthesized porphyrins only after the addition of delta-aminolevulinic acid to their medium, while proplastids of still other strains could not synthesize porphyrins from this precursor. Normal cells when grown in total darkness were found to possess proplastids morphologically identical with those of the bleached strains. Upon exposure to light the proplastids enlarged and greened. In cells which grew under continuous light the plastid system appeared as an interconnected system of tubules. Cells maintained on a schedule of 12-hour light and 12-hour dark had plastids which were detached from each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02628.x
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  • 2
    Electronic Resource
    Electronic Resource
    Life and death (1985)
    [s.l.] : Nature Publishing Group
    Nature 315 (1985), S. 626-626 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR - Rapid innovations in the biological sciences and technology are creating new and difficult ethical problems, as is evident to readers of Nature from numerous articles and letters. Most prominent are problems concerned with the early phase of the human life cycle. (See for example recent ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/315626b0
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  • 3
    Electronic Resource
    Electronic Resource
    Evidence for role of flagella as sensory transducers in mating of Chlamydomonas reinhardi (1977)
    [s.l.] : Nature Publishing Group
    Nature 265 (1977), S. 444-445 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The mating process commences following the mixing of ( + ) and (-) gametes of Chlamydomonas reinhardi. Several physiological changes have been observed. These include cell wall lysis4-6 and fertilisation tubule formation in the ( + ) gametes5,6. We have previously reported the accumulation of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/265444a0
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  • 4
    Electronic Resource
    Electronic Resource
    The relationship between tonicity and flagellar length (1978)
    [s.l.] : Nature Publishing Group
    Nature 275 (1978), S. 651-652 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To determine what effect osmotic pressure has on flagellar length, vegetative cells of Chlamydomonas reinhardi were incubated in two salt solutions: sodium chloride and sodum pyrophosphate, and two sugar solutions: mannitol and sucrose, all at a tonicity approximately three times that of normal ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/275651a0
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  • 5
    Electronic Resource
    Electronic Resource
    Biotransformation of alkanes and haloalkanes by a marine amoeba (1991)
    Springer
    Applied microbiology and biotechnology 34 (1991), S. 814-817 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A marine amoeba, Trichosphaerium I-7, originally found feeding on macroalgae in a region of natural oil seepage, was maintained in the laboratory for prolonged periods on hexadecane, octadecane, 1-chlorooctadecane, or 1-bromooctadecane as a carbon source. The cells attached readily and eroded holes in thin layers of these compounds. Crystalline and clear spherical inclusions appeared in the cytoplasm of cells fed these xenobiotics followed by a marked cell darkening. Thin layer chromatographic analyses of acetone extracts from amoebae grown for 12 days on [1-14C]octadecane demonstrated the formation of labelled substances of higher polarity than the original alkane. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography revealed that 14C derived from [1-14C]octadecane was incorporated into acetone-insoluble macromolecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169356
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  • 6
    Electronic Resource
    Electronic Resource
    Calluses and callus-like growth in seaweeds: Induction and culture (1987)
    Springer
    Hydrobiologia 151-152 (1987), S. 131-138 
    Details
    ISSN: 1573-5117
    Keywords: seaweed ; calluses ; induction ; culture ; differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00046118
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  • 7
    Electronic Resource
    Electronic Resource
    Microorganisms as digestors of seaweed cell walls (1987)
    Springer
    Hydrobiologia 151-152 (1987), S. 405-409 
    Details
    ISSN: 1573-5117
    Keywords: seaweed ; seaweed pathogens ; cell wall digestion ; seaweed-digesting amoeba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00046160
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of viability and cell types of macroalgal protoplasts using flow cytometry (1995)
    Springer
    Journal of applied phycology 7 (1995), S. 413-420 
    Details
    ISSN: 1573-5176
    Keywords: protoplasts ; flow cytometry ; seaweed ; biotechnology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ability to rapidly distinguish viable sub-populations of cells within populations of macroalgal protoplast isolations was demonstrated using flow cytometry. Viable protoplasts from Ulva sp. and Porphyra perforata J. Ag. were distinguished from non-viable protoplasts based on differential fluorescein accumulation. The identities of cortical and epidermal protoplasts from Macrocystis pyrifera (L.) C. Ag. were inferred based on light-scattering and chlorophyll a autofluorescence. Three cell types could be distinguished among protoplasts released from thalli of P. perforata based on chlorophyll a and phycoerythrin autofluorescence. Mixed protoplast populations of Ulva sp. and P. perforata were also discernable based on relative chlorophyll a and phycoerythrin autofluorescence. The ability to screen heterogenous protoplast populations rapidly, combined with the cell sorting capabilities of many flow cytometers, should prove valuable for seaweed biotechnology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00003799
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of LiCl on compression and tension properties ofPophyra perforata tissue (1996)
    Springer
    Journal of applied phycology 8 (1996), S. 247-252 
    Details
    ISSN: 1573-5176
    Keywords: compression ; LiCl ; Porphyra ; seaweed ; tension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mechanical properties of various differentiated regions of thePorphyra perforata thallus and the effect of LiCl were studied by performing compression and tension tests. Among the various differentiated tissues, the holdfast area was high in its ‘compressive modulus of elasticity’ and ‘tensile modulus of elasticity’, possibly related to its thick matrix. Vegetative non-dividing tissue and vegetative dividing tissue were the most flexible and strong, showing the highest ‘percentage elongation at break’ and ‘tensile strength’. The patch area, which is a transition zone leading to sexually mature tissue, had moderate values of tensile properties. Meanwhile, sexually differentiated male and female tissues had the highest ‘compressive modulus of elasticity’ and lowest tensile properties. Thes tisues tended to crumble easily. Treatments in LiCl, as used for DNA extraction, resulted in a decrease in both ‘compressive modulus of elasticity’ (87%) and ‘tensile modulus of elasticity’ (54%). After treatment of tissue for chromosome staining in a method using LiCl, there was a marked decrease in ‘tensile modulus of elasticity’ (49%), while the ‘compressive modulus of elasticity’ remained unchanged. Such mechanical changes verify the softening effect of LiCl on the seaweedP. perforata tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184978
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  • 10
    Electronic Resource
    Electronic Resource
    DNA extraction conditions fromPorphyra perforata using LiCl (1995)
    Springer
    Journal of applied phycology 7 (1995), S. 101-107 
    Details
    ISSN: 1573-5176
    Keywords: DNA extraction ; LiCl ; PCR ; Porphyra perforata ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rapid and economical method of DNA extraction from a red seaweedPorphyra perforata J. Agardh has been developed by the use of lithium chloride. This paper describes the optimization of extraction conditions. Heat treatment of tissues in a solution (0.8 M LiCl, 0.6% Sarkosyl, 10 mM EDTA, 0.2% PVPP, 5% ß-mercaptoethanol, pH 9.0) at 55 °C for 10 min extracts DNA that is of sufficient quality to be used as a template for PCR amplification. Total DNA yield was approximately 30 to 50μg g−1 t of partially dried tissue. Total RNA yield was approximately 400μg g−1 of partially dried tissue. Carbohydrate was contained as approximately 40 to 90 mM (expressed as glucose equivalents) from 1 g tissue, and protein contamination calculated as the O.D. 260/280 ratio was in the range of 1.4 to 1.7. The DNA was characterized by high molecular weight larger than 50 kb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00693055
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