ISSN:
1365-2761
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Abstract. Two new viruses, goldfish virus-1 (GFV-1) and goldfish virus-2 (GFV-2), were isolated from swimbladder cultures prepared from healthy Carassius auratus (L.). The isolates were sensitive to pH, heat, and chloroform but stable to freezing and thawing. Treatment with IUDR, Feulgen staining and acridine orange staining indicated that the viruses contain double-stranded DNA. Electron microscopy revealed enveloped virions of 180 nm enclosing icosahedral capsids of 120 nm. Viral growth was studied by light and electron microscopy and infectivity assays. Virions were assembled in cytoplasmic inclusion bodies and apparently released through cell degeneration. Infectious virus was first produced at 24 h post-infection. Progressive cytopathology was observed leading to cell ghosts by 96 h post-infection. The results suggest tentative placement of the viruses in the family Iridoviridae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2761.1983.tb00104.x
Permalink