Publication Date:
2001-02-27
Description:
Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing gamma-tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures-distinct from elements of the microtubule-organizing center-that is required for the somatic cell cycle to progress through G1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hinchcliffe, E H -- Miller, F J -- Cham, M -- Khodjakov, A -- Sluder, G -- R01 GM059363/GM/NIGMS NIH HHS/ -- R01 GM059363-02/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Feb 23;291(5508):1547-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Laboratory of Cell Regulation, Wadsworth Center, Albany, NY 12201, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11222860" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens/metabolism
;
Cell Division/drug effects
;
Cell Line
;
Centrioles/physiology
;
Centrosome/*physiology
;
Cercopithecus aethiops
;
Cytoplasmic Granules/physiology/ultrastructure
;
*G1 Phase
;
Interphase
;
Microscopy, Video
;
Microtubule-Organizing Center/physiology
;
Microtubules/physiology/ultrastructure
;
Mitosis
;
Paclitaxel/pharmacology
;
*S Phase
;
Spindle Apparatus/physiology/ultrastructure
;
Tubulin/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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