Publication Date:
2001-02-07
Description:
Long-range regulatory elements are difficult to discover experimentally; however, they tend to be conserved among mammals, suggesting that cross-species sequence comparisons should identify them. To search for regulatory sequences, we examined about 1 megabase of orthologous human and mouse sequences for conserved noncoding elements with greater than or equal to 70% identity over at least 100 base pairs. Ninety noncoding sequences meeting these criteria were discovered, and the analysis of 15 of these elements found that about 70% were conserved across mammals. Characterization of the largest element in yeast artificial chromosome transgenic mice revealed it to be a coordinate regulator of three genes, interleukin-4, interleukin-13, and interleukin-5, spread over 120 kilobases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Loots, G G -- Locksley, R M -- Blankespoor, C M -- Wang, Z E -- Miller, W -- Rubin, E M -- Frazer, K A -- AI30663/AI/NIAID NIH HHS/ -- GM-5748202/GM/NIGMS NIH HHS/ -- HL56385/HL/NHLBI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 2000 Apr 7;288(5463):136-40.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Genome Sciences Department, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10753117" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Chromosomes, Human, Pair 5/genetics
;
Conserved Sequence
;
*DNA-Binding Proteins
;
Fungal Proteins/biosynthesis/genetics
;
Gene Expression Regulation
;
Humans
;
Interleukin-13/biosynthesis/*genetics
;
Interleukin-4/biosynthesis/*genetics
;
Interleukin-5/biosynthesis/*genetics
;
Kinesin/biosynthesis/genetics
;
Mice
;
Mice, Transgenic
;
Physical Chromosome Mapping
;
*Regulatory Sequences, Nucleic Acid
;
*Saccharomyces cerevisiae Proteins
;
Species Specificity
;
Th1 Cells/immunology
;
Th2 Cells/immunology
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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