ISSN:
1574-6941
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
The particulate methane monooxygenase genes (pMMO) were amplified from methane-biostimulated aquifer samples using two sets of primers, A189 plus A682 and pmof2 plus pmor, and the products were cloned and sequenced. The analysis of the sequences revealed a high diversity of pMMO genes in the aquifers. Some of the pMMO gene sequences (LP20 and LP21) isolated in this study were unique and unrelated to methanotrophs previously isolated from this site. Throughout all the samples collected from the different methane biostimulation trials, competitive PCR amplification using specific pMMO primers revealed that type I methanotrophs predominated over type II methanotrophs. The partial soluble methane monooxygenase genes were also amplified from the aquifer samples using specially designed primers but sequencing revealed a lower diversity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6941.2001.tb00894.x
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