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Evaluation of Ascorbic Acid as a Quorum-sensing Analogue to Control Growth, Sporulation, and Enterotoxin Production in Clostridium perfringens (2004)

NOVAK, J. S. ; FRATAMICO, P. M.

Oxford, UK : Blackwell Publishing Ltd

Journal of food science 69 (2004), S. 0

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ISSN: 1750-3841

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology

Notes: : Inhibition of quorum sensing measured by autoinducer-2 (AI-2) activity was investigated in the presence and absence of ascorbic acid, an AI-2 analogue. Subsequent effects on AI-2 production, as well as growth, sporulation, and enterotoxin (C. perfringens enterotoxin [CPE]) production in Clostridium perfringens were examined. The addition of ascorbic acid to supernatants from ground beef resulted in a 100-fold decrease in AI-2 activity. The addition of sodium ascorbate, a nonacidic salt of ascorbic acid, also resulted in AI-2 assay inhibition. Spore production decreased in the presence of ascorbic acid. Western immunoblot analyses showed that CPE levels were highest after 24 h without ascorbic acid. This study explored the unique concept of signal inhibition to control pathogens in food.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb13374.x

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Simultaneous detection of Salmonella spp and Escherichia coli O157:H7 by multiplex PCR (1998)

Fratamico, P M ; Strobaugh, T P

Springer

Journal of industrial microbiology and biotechnology 21 (1998), S. 92-98

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ISSN: 1476-5535

Keywords: Keywords: immunomagnetic separation; bovine feces; carcass wash water; apple cider; ground beef

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Contamination of foods with pathogens such as Escherichia coli O157:H7 and Salmonella is a major concern worldwide and rapid, sensitive, and reliable methods are needed for detection of these organisms. Since these pathogens can contaminate similar foods and other types of samples, a multiplex polymerase chain reduction (PCR) was designed to allow simultaneous detection of both E. coli O157:H7 and Salmonella spp directly from enrichment cultures. Samples of apple cider, beef carcass wash water, ground beef, and bovine feces were inoculated with both E. coli O157:H7 and S. typhimurium at various bacterial levels. Following enrichment culturing for 20–24 h at 37°C in modified EC broth or buffered peptone water both containing novobiocin, the samples were subjected to a DNA extraction technique or to immunomagnetic separation then tested by the multiplex PCR assay. Four pairs of primers were employed in the PCR: primers for amplification of E. coli O157:H7 eaeA, stx 1/2 and plasmid sequences and for amplification of a portion of the Salmonella invA gene. Four fragments of the expected sizes were amplified in a single reaction and visualized following agarose gel electrophoresis in all the samples inoculated with ≤ 1 CFU g−1 or ml−1. Results can be obtained in approximately 30 h. The multiplex PCR is a potentially powerful technique for rapid and sensitive co-detection of both pathogens in foods and other types of samples.