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  • 1
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    Molecular bases of the interactions between luteoviruses and aphids * (1996)
    EDP Sciences
    Details
    Publication Date: 1996-01-01
    Print ISSN: 0249-5627
    Electronic ISSN: 1297-9643
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by EDP Sciences
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    PAPER CURRENT
  • 2
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    Diversity of luteoviruses infecting faba bean (Vicia faba L) in Morocco, and their detection by the polymerase chain reaction (1996)
    EDP Sciences
    Details
    Publication Date: 1996-01-01
    Print ISSN: 0249-5627
    Electronic ISSN: 1297-9643
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by EDP Sciences
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  • 3
    Electronic Resource
    Electronic Resource
    Identification and properties of a deviant isolate of the broad bean yellow band serotype of pea early-browning virus from faba bean (Vicia faba) in Algeria (1992)
    Springer
    European journal of plant pathology 98 (1992), S. 237-252 
    Details
    ISSN: 1573-8469
    Keywords: chickpea ; hypersensitivity ; lentil ; Nicotiana rustica ; N. tabacum ; Phaseolus vulgaris ; Pisum sativum ; seed transmission ; soil-borne virus ; strain differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A virus, isolated from faba bean (Vicia faba) obtained from Algeria, was readily recognized as a tobravirus by its particle sizes and morphology. Pea (Pisum sativum) and French bean (Phaseolus vulgaris) characteristically reacted to the isolate like pea early-browning virus (PEBV), but faba bean,Antirrhinum majus, Nicotiana rustica, andN. tabacum reacted with line-pattern symptoms which were unusually brilliant on theNicotiana species. In electronmicroscope decoration tests, the isolate did not react with an antiserum to the Dutch type strain of PEBV, but with one to the broad bean yellow band (BBYB) serotype from Italy. It resembles this serotype in reaction on faba bean, but seems to differ appreciably onN. rustica, N. tabacum, andPetunia hybrida. It is described as a deviant isolate of the BBYB serotype of PEBV. All thirteen faba-bean genotypes tested were found to be susceptible to the Algerian isolate and two Dutch type strain isolates of the virus, and to react with erratic line-pattern symptoms to the Algerian isolate only. All ten genotypes of chickpea (Cicer arietinum) tested reacted hypersensitively, and four out of ten genotypes of lentil (Lens culinaris) were susceptible to the virus but reacted differentially to the three isolates. Seed transmission of PEBV, including the new isolate, in faba bean is confirmed (9% for the Algerian isolate, and over 45% for one of the Dutch type strain isolates), and seed transmission of the virus in a non-legume (N. rustica, 4%) is herewith first reported. This is the first report on the occurrence of the BBYB serotype of PEBV outside Italy, and of PEBV outside Morocco in North Africa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02000091
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  • 4
    Electronic Resource
    Electronic Resource
    A mild strain of broad bean mottle virus from faba bean (Vicia faba L.) in the Sudan (1992)
    Springer
    European journal of plant pathology 98 (1992), S. 253-256 
    Details
    ISSN: 1573-8469
    Keywords: cross-protection ; virus variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new strain of broad bean mottle virus, isolated from faba bean (Vicia faba L.) in the Sudan, is described. It differs considerably from known isolates by its nearly symptomless infection of faba bean in spite of high concentrations of the virus in infected plants. It does not differ from regular isolates in gel-diffusion serology, light and electron microscopy, host range and symptoms in major hosts other than faba bean. It may constitute a potential threat to other food legumes in the region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02000092
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  • 5
    Electronic Resource
    Electronic Resource
    Broad bean mottle virus in Morocco; curculionid vectors, and natural occurrence in food legumes other than faba bean (Vicia faba) (1993)
    Springer
    European journal of plant pathology 99 (1993), S. 219-226 
    Details
    ISSN: 1573-8469
    Keywords: Apion ; Cicer arietinum ; Curculionidae ; Hypera ; Lens culinaris ; Pachytychius ; Phaseolus vulgaris ; Pisum sativum ; Sitona ; Smicronyx ; weevil transmission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Broad bean mottle virus (BBMV) was transmitted from infected to healthy faba-bean plants by the curculionid weevilsApion radiolus Kirby,Hypera variabilis Herbst,Pachytychius strumarius Gyll,Smicronyx cyaneus Gyll, andSitona lineatus L. The latter appeared to be an efficient vector: acquisition and inoculation occurred at the first bite, the rate of transmission was c. 41%, and virus retention lasted for at least seven days.S. lineatus transmitted the virus from faba bean to lentil and pea, but not to the three genotypes of chickpea tested. This is the first report on the generaHypera, Pachytychius, andSmicronyx as virus vectors, and onA. radiolus, H. variabilis, P. strumarius, andS. cyaneus as vectors of BBMV. Out of 351 samples of food legumes with symptoms suggestive of virus infection, 16, 11, 19, and 17% of the samples of chickpea, lentil, pea, and common bean, respectively, were found infected when tested for BBMV in DAS-ELISA. This is the first report on the natural occurrence of BBMV in chickpea, lentil, pea, and common bean. The virus should be regarded as a food-legume virus rather than a faba-bean virus solely, and is considered an actual threat to food legume improvement programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974666
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular evidence for the occurrence of beet western yellows virus on chickpea in Morocco (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 481-484 
    Details
    ISSN: 1573-8469
    Keywords: food legumes ; Cicer arietinum L. ; luteovirus ; PCR ; nucleotide sequence ; coat protein gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A luteovirus isolate infecting chickpea in Morocco was experimentally transmitted by Myzus persicae to Physalis floridana, on which it produced mild symptoms. When tested in western blots against antisera to known legume luteoviruses, this isolate reacted strongly to beet western yellows virus (BWYV) antiserum, moderately to bean leafroll virus antiserum, while no reaction was recorded with the antiserum against subterranean clover red leaf virus. In PCR, a fragment of ca. 950 bp was amplified, comprising the 3' end of the open reading frame (ORF) 3, the complete coat protein gene (ORF 4), and the non-translated region in between these ORFs. The nucleotide sequence of the amplified fragment showed high similarity with BWYV (approximately 96%), and lower (50–60%) with other luteoviruses reported to infect legumes. On the basis of these data, the Moroccan isolate was identified as BWYV. This is the first molecular evidence for the occurrence of BWYV on chickpea in Morocco, and on food legumes in general in North Africa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008687629522
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  • 7
    Electronic Resource
    Electronic Resource
    Broad bean mottle virus in Morocco; variability, interaction with food legume species, and seed transmission in faba bean, pea, and chickpea (1992)
    Springer
    European journal of plant pathology 98 (1992), S. 329-342 
    Details
    ISSN: 1573-8469
    Keywords: sensitivity ; susceptibility ; pathogenicity ; vulnerability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Biological indexing of faba-bean samples collected during an earlier virus survey in Morocco revealed variation in symptom severity among isolates of broad bean mottle virus (BBMV). When seven selected isolates from Morocco and three from Algeria, Sudan, and Tunisia were further compared, they could be divided into mild, severe, and intermediate isolates, according to their pathogenicity on a number of food-legume genotypes tested. The Moroccan isolate SN1 and the Sudanese SuV256 were very mild, and deviant also in their effect onGomphrena globosa, whereas the Tunisian TV75-85 and the Moroccan VN5 were virulent. Representative isolates were indistinguishable, however, in coat-protein molecular weights, and they reacted similarly to the antisera to a Moroccan and a Syrian isolate in electro-blot immunoassay. Promising ICARDA breeding lines and accessions—ten each of pea and lentil, nine of chickpea, and twelve of faba bean-were all found vulnerable (susceptible and sensitive) to all isolates. Within each food-legume species, vulnerability varied from high to moderate, and no immunity was detected. Virus concentrations in faba-bean lines suggest that isolates differ in virulence rather than in aggressiveness, and that the differences in vulnerability among the lines are due to differences in sensitivity rather than in susceptibility. When pooled seed samples were germinated and seedlings were tested for BBMV in DASELISA, the virus was found seed-transmitted in faba bean, chickpea, and pea at transmission rates of ca 1.2, 0.9, and 0.1%, respectively. This is the first report on seed transmission of BBMV in faba bean, when occurring on its own, and the first record of such seed transmission in chickpea and pea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974461
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