ISSN:
1573-5028
Keywords:
Zea mays L.
;
transformation
;
electroporation
;
bar
;
phosphinothricin acetyltransferase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid containing the bar gene, resulted in high-frequency recovery of stably transformed callus lines. Plants were regenerated from thirteen transformed callus lines and transmission of bar to progeny was demonstrated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040573
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