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  • 1
    Electronic Resource
    Electronic Resource
    Plant protein secretion on tap (1999)
    [s.l.] : Nature America Inc.
    Nature biotechnology 17 (1999), S. 427-427 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Transgenic plants have shown tremendous potential as photosynthetic bioreactors for the production of foreign proteins such as antibodies and streptavidin. But harvesting these recombinant molecules can be problematic because they typically are sequestered inside plant cells and require tissue ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/8594
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  • 2
    Electronic Resource
    Electronic Resource
    Initiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.) (1989)
    Springer
    Plant cell reports 8 (1989), S. 203-206 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic callus and suspension cultures of eastern white pine (Pinus strobus) have been obtained. The whole female gametophyte was plated on a medium containing 50 mg/l glutamine, 500 mg/l casein hydrolysate, 3% sucrose, 2 mg/1 2,4-D, 1 mg/1 BA and 0.2% Gelrite as a solidifying agent. Embryogenic calli could be seen as early as 5 days following culture. Histological studies indicate proliferation of pre-existing embryogenic tissue in the corrosion cavity followed by extrusion of embryogenic callus through the micropylar end of the gametophyte. Embryogenic suspension cultures were obtained by placing embryogenic callus into liquid medium. Embryogenic suspension cultures were subcultured weekly and proliferated as early-stage embryos with attached suspensors. Embryo development was obtained following transfer of the embryogenic tissue to an auxin-free medium containing 50 mM glutamine, 38 μM abscisic acid, and 6% sucrose. Although embryo development could be consistently obtained, whole plants have not yet been recovered from these somatic embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00778532
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  • 3
    Electronic Resource
    Electronic Resource
    Initiation of callus and somatic embryos from explants of mature cotton (Gossypium klotzschianum Anderss) (1984)
    Springer
    Plant cell reports 3 (1984), S. 41-43 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Stem and petiole sections from a diploid species of cotton (Gossypium klotzschianum Anderss.) were longitudinally sliced and placed on a callus induction medium. Explants with the cut surface in contact with the medium either browned or produced a slow growing red callus which could not be subcultured. Explants oriented with the epidermis in contact with the medium produced a rapidly growing, friable, green callus which could be subcultured biweekly and has been maintained for 12 months. Globular and torpedo embryos were obtained 2 weeks after transfer of the callus to a liquid medium containing the auxin, 2,4-dichlorophenoxyacetic acid. Following subculture to an auxin-free medium, the embryos underwent additional development but plantlets were not obtained. Attempts to consistently regenerate plants from embryos are currently under way.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270228
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  • 4
    Electronic Resource
    Electronic Resource
    Growth and disinfestation of 6 different bacteria in embryogenic suspension cultures of cotton (1991)
    Springer
    Plant cell reports 10 (1991), S. 380-383 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Growth of 6 different common laboratory bacteria (Escherichia coli, Flavobacterium balustrum, Xanthomonas maltophilia, Enterobacter cloacae, Pseudomonas fluorescens, and Agrobacterium tumefaciens) in a bacterial medium, fresh plant medium, and “spent” plant media was initially measured. In all cases, bacteria grew best in the bacterial medium followed by the fresh plant medium. The spent plant medium did not support growth of the bacteria and apparently was actively toxic to bacterial cells. Proliferating, embryogenic suspension cultures of cotton (Gossypium hirsutum) were then inoculated with these 6 different bacteria. Two to three d following bacterial inoculation, embryogenic tissues were placed in various concentrations of bleach for various amounts of time, rinsed with sterile water, and placed on a bacterial culture medium. Clumps of embryogenic tissue which showed no visible bacterial growth after 3 d of culture were then transferred to an agar-solidified plant tissue culture medium to determine viability of bleachdisinfested tissues. Viable, single pieces of the disinfested embryogenic tissue were then used to reinitiate embryogenic suspension cultures. Treatment of contaminated tissue with a 1% bleach solution for 1–5 min resulted in the highest recovery of viable, disinfested tissues using 5 of the 6 bacteria. It was not possible to remove F. balustrum from clumps of embryogenic tissue without also killing the plant tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232605
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  • 5
    Electronic Resource
    Electronic Resource
    Transformation of 12 different plasmids into soybean via particle bombardment (1996)
    Springer
    Plant cell reports 15 (1996), S. 500-505 
    Details
    ISSN: 1432-203X
    Keywords: Glycine max ; Recombination ; Soybean ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Particle bombardment offers a simple method for the introduction of DNA into plant cells. Multiple DNA fragments may be introduced on a single plasmid or on separate plasmids (co-transformation). To investigate some of the properties and limits of co-transformation, 12 different plasmids were introduced into embryogenic suspension culture tissue of soybean [Glycine max (L.) Merrill] via particle bombardment. The DNAs used for co-transformation included 10 plasmids containing KFLP markers for maize and 2 plasmids separately encoding hygromycin-resistance and ß-glucuronidase. Two weeks following bombardment with the 12 different plasmids, suspension culture tissue was placed under hygromycin selection. Hygromycin-resistant clones were isolated after an additional 5 to 6 weeks. Southern hybridization analysis of 26 hygromycin-resistant embryogenic clones verified the presence of introduced plasmid DNAs. All of the co-transforming plasmids were present in most of the transgenic soybean clones and there was no preferential uptake and integration of any of the plasmids. The copy number of individual plasmids was approximately equal within clones but highly variable between clones. While some clones contained as few as zero to three copies of each plasmid, others clones contained as many as 10 to 15 copies of each of the 12 different plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232982
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  • 6
    Electronic Resource
    Electronic Resource
    Development of the particle inflow gun for DNA delivery to plant cells (1992)
    Springer
    Plant cell reports 11 (1992), S. 323-328 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple and inexpensive particle bombardment device was constructed for delivery of DNA to plant cells. The Particle Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles using pressurized helium in combination with a partial vacuum. The particles are accelerated directly in a helium stream rather than being supported by a macrocarrier. Bombardment parameters were partially optimized using transient expression assays of a ß-glucuronidase gene in maize embryogenic suspension culture and cowpea leaf tissues. High levels of transient expression of the ß-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of corn and soybean, and leaf tissue of cowpea. Stable transformation of embryogenic tissue of soybean has also been obtained using this bombardment apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233358
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  • 7
    Electronic Resource
    Electronic Resource
    Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize (1993)
    Springer
    Plant cell reports 12 (1993), S. 84-88 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of osmotic conditioning on both transient expression and stable transformation were evaluated by introducing plasmid DNAs via particle bombardment into embryogenic suspension culture cells of Zea mays (A188 × B73). Placement of cells on an osmoticum-containing medium (0.2 M sorbitol and 0.2 M mannitol) 4 h prior to and 16 h after bombardment resulted in a statistically significant 2.7-fold increase in transient ß-glucuronidase expression. Under these conditions, an average of approximately 9,000 blue foci were obtained from 100 μl packed cell volume of bombarded embryogenic tissue. Osmotic conditioning of the target cells resulted in a 6.8-fold increase in recovery of stably transformed maize clones. Transformed fertile plants and progeny were obtained from several transformed cell lines. We believe the basis of osmotic enhancement of transient expression and stable transformation resulted from plasmolysis of the cells which may have reduced cell damage by preventing extrusion of the protoplasm from bombarded cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00241940
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  • 8
    Electronic Resource
    Electronic Resource
    Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.) (1996)
    Springer
    Plant cell reports 15 (1996), S. 489-494 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report a strength comparison of a large variety of monocot and dicot intron-containing fragments inserted in the 5′ untranslated leader, between the CaMV 35S promoter and the uidA gene (coding for the ß-glucuronidase: GUS). Relative strengths of the intron-containing fragments were evaluated by comparing transient GUS expression after particle bombardment in embryogenic maize and bluegrass suspension cultures. Our results confirm a dramatic dependence on the presence of an intron for chimeric gene expression in both species. On average, the maize first intron of ubi1 provided the highest enhancement of gene expression in maize and bluegrass (71- and 26-fold enhancement, respectively). Half of the introns tested affected gene expression differently in bluegrass and maize. This suggests that the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots. We also report enhancement of gene expression (92-fold) in a monocot species by a dicot intron (chsA intron).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232980
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  • 9
    Electronic Resource
    Electronic Resource
    Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.) (1996)
    Springer
    Plant cell reports 15 (1996), S. 489-494 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We report a strength comparison of a large variety of monocot and dicot intron-containing fragments inserted in the 5' untranslated leader, between the CaMV 35S promoter and the uidA gene (coding for the β-glucuronidase: GUS). Relative strengths of the intron-containing fragments were evaluated by comparing transient GUS expression after particle bombardment in embryogenic maize and bluegrass suspension cultures. Our results confirm a dramatic dependence on the presence of an intron for chimeric gene expression in both species. On average, the maize first intron of ubil provided the highest enhancement of gene expression in maize and bluegrass (71- and 26-fold enhancement, respectively). Half of the introns tested affected gene expression differently in bluegrass and maize. This suggests that the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots. We also report enhancement of gene expression (92-fold) in a monocot species by a dicot intron (chsA intron).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050060
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  • 10
    Electronic Resource
    Electronic Resource
    Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill] (1988)
    Springer
    Plant cell reports 7 (1988), S. 238-241 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryos and embryogenic tissues were initiated from immature zygotic embryos of soybean [Glycine max (L.) Merrill cv. ‘Fayette’]. Zygotic embryos were placed on a medium containing 40 mg/l of 2,4-dichlorophenoxyacetic acid and 6% sucrose. Somatic embryos were first seen 4 weeks after cultures were initiated. Following transfer, secondary somatic embryos proliferated directly from the apical or terminal portions of the older primary somatic embryos. Single somatic embryos or clusters of embryos were seen growing directly from the top of older somatic embryos. Light microscopy revealed that these embryos were of surface or subsurface origin. The apical soybean somatic embryo tissue may represent cotyledonary tissue (which has been shown to be most responsive) at a very young and manipulatable state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272532
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