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  • 1
    Electronic Resource
    Electronic Resource
    Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 16 (1995), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In the presence of toluene, xylenes and other structural analogues, the regulatory protein XylR, of the family of transcriptional regulators which act in concert with the σ54 factor, activate the promoter Pu of the TOL (toluene degradation) plasmid pWW0 of Pseudomonas putida. Amino acid changes Val-219-Asp and Ala-220-Pro, introducing a proline kink at the hinge region between the N-terminal A domain and the central portion of XylR, resulted in a semi-constitutive phenotype which mimicked the activating effect of aromatic inducers. This phenotype was further exacerbated by inserting extra amino acid residues within the same inter-domain region. A truncated XylR protein devoid of the signal-receiving, amino-terminal portion of the protein stimulated the cognate promoter Pu at high levels independently of inducer addition, both in Escherichia coli and in Pseudomonas putida. Replacement of the amino-terminal domain by a heterologous peptide derived from the MS2 virus polymerase resulted in a hybrid protein still able to bind DNA to the same extent in vivo as XylR, but unable to stimulate transcription. These data indicate that a key event in the activation of XyIR by toluene/xylenes is the release of the repression caused by the A domain of the protein on surfaces located at the central domain of the regulator.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1995.tb02293.x
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  • 2
    Electronic Resource
    Electronic Resource
    The Bacillus subtilis chromatin-associated protein Hbsu is involved in DNA repair and recombination (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 23 (1997), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The Bacillus subtilis hbs gene encodes an essential chromatin-associated protein termed Hbsu. Hbsu, the counterpart of the Escherichia coli HU protein, binds DNA in a non-specific way but has a clear preference for bent, kinked or altered DNA sequences. To investigate the role of Hbsu in DNA repair and DNA recombination we have constructed a series of site-directed mutants in the hbs gene and used these mutant genes to substitute the wild-type chromosomal hbs gene. The hbs47 mutation, which codes for a mutant protein in which residue Phe-47 has been replaced by Trp, does not cause any discernible phenotype. Additional substitution of residue Arg-55 by Ala (hbs4755 mutation) rendered cells deficient in DNA repair, homologous recombination and (i protein-mediated site-specific recombination. We have also tested the effect on DNA repair of the hbs4755 mutation in combination with mutations in different functions of homologous DNA recombination (recA, recF, recG, recti and addAB). The hbs4755 mutation did not modify the sensitivity of recH and addAB cells to the DNA-damaging agents methylmethane sulphonate (MMS) or 4-nitroquinoline-1-oxide (4NQO), and it only marginally affected recF and recG cells. The hbs4755 mutation blocked intermolecular recombination in recH cells and markedly reduced it (20- to 50-fold) in recF and recG cells, but had no effect on addAB cells. Taken together, these data indicate that the Hbsu protein is required for DNA repair and for homologous DNA recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1997.3061670.x
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  • 3
    Electronic Resource
    Electronic Resource
    On the instability region around the 5 : 3 Jovian resonance (1988)
    Springer
    Earth, moon and planets 43 (1988), S. 227-232 
    Details
    ISSN: 1573-0794
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract From the classical Fourier expansion of the disturbing function for an asteroid under the effects of the 5 : 3 Jovian resonance (in the planar elliptic problem), a system of differential equations in (h, k) variables with periodic coefficients was constructed. This system was solved analytically using Baker's matricial method, and the characteristic exponents calculated. Results show the existence of an instability region (in the semi-major axis domain) around this resonance, even though it is smaller than the observed extension of the gap.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00117095
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  • 4
    Electronic Resource
    Electronic Resource
    Phase Diagram in Asteroidal Motion (1997)
    Springer
    Earth, moon and planets 77 (1997), S. 73-81 
    Details
    ISSN: 1573-0794
    Keywords: Asteroids ; resonance ; Kirkwood gaps ; celestial mechanics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In order to study the dynamical behaviour of asteroids in commensurability with a planet, we propose a phase diagram obtained by short computer time. We test this numerical procedure by analyzing the behaviour of real and fictitious asteroids in first order commensurabilities with Jupiter. We have also studied the evolution time of the orbital elements and other variables to compare these results with those obtained in the phase diagram. The results obtained with our numerical technique were compared to similar results previously obtained by other authors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006136826250
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization and properties of steroid binding protein in Bufo arenarum serum (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 38 (1994), S. 364-372 
    Details
    ISSN: 1040-452X
    Keywords: Steroids ; Binding ; Amphibian serum proteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Serum steroid binding properties of mature Bufo arenarum females were studied. Binding data obtained using charcoal adsorption assay and equilibrium dialysis methods indicates a single protein, named Bufo arenarum sex binding protein (Ba SBP), which binds 5 α-dihydrotestosterone (DHT), testosterone (T), and estradiol-17β (E2) with high affinity (107 M-1 - 108 M-1) and fair capacity (10-6 M). Scatchard plot analysis demonstrated the coexistence of two binding sites. Ba SBP has a sedimentation coefficient of 5.2 S in sucrose gradient centrifugation in low salt and under steady-state conditions. The specificity of this protein, determined by competitive binding experiments, is comparable to human SBP. DHT and T bind with higher affinity than E2. Estriol and estrone competed poorly, while diethylstilbestrol and C21 steroids did not compete. The binding capacity of this protein is under estrogenic control. © 1994 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080380403
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  • 6
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    The Ebro river in the 20th century or the ecomorphological transformation of a large and dynamic Mediterranean channel (2011)
    Wiley
    Details
    Publication Date: 2011-11-10
    Description: In the first decades of the 20th century, the Ebro River was the Iberian channel with the most active fluvial dynamics and the most remarkable spatial-temporal evolution. Its meandering typology, the dimensions of its floodplain, and the singularities of its flow regime produced an especially interesting set of river functions. The largest dynamics of the Ebro River are concentrated along the meandering profile of the central sector. During the 20th century, this sector experienced a large alteration of its geomorphological structure. We present here an analysis of this evolution through the cartographic study of a long segment of the river (~250 km) in 1927, 1956 and 2003. The results show a large reduction in bank sinuosity, a progressive loss of fluvial territory, and a large decrease in channel width. These changes are especially clear in the areas previously most ecologically connected with the active channel. The fluvial territory of the river in 2003 was approximately half that found during the first decades of the 20th century. Forest plantations, which were non-existent in 1927, occupied more than 1,500 ha of the study area in the last decade. This intense geomorphological transformation becomes ecologically visible in (i) a 35% reduction of the area occupied by riparian vegetation; (ii) a loss of the heterogeneity of riparian forest spots, which were formerly structured in an irregular mosaic far from the river thalweg; and (iii) a modification of the riparian forest structure, which is currently linear, uniform, thin and very close to the river axis. The ecomorphological alteration was intensified by the remarkable reduction in bank length (13%) and the reduced dynamism of the present river system, indicated by an increase in the percentage of fluvial territory occupied by riparian forests and a reduction in the area occupied by the active channel. Copyright © 2011 John Wiley & Sons, Ltd.
    Print ISSN: 0197-9337
    Electronic ISSN: 1096-9837
    Topics: Geography , Geosciences
    Published by Wiley
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  • 7
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    The Ebro River in the 20th century or the ecomorphological transformation of a large and dynamic Mediterranean channel (2012)
    Wiley
    Details
    Publication Date: 2012-04-15
    Description: ABSTRACT In the first decades of the 20th century, the Ebro River was the Iberian channel with the most active fluvial dynamics and the most remarkable spatial-temporal evolution. Its meandering typology, the dimensions of its floodplain, and the singularities of its flow regime produced an especially interesting set of river functions. The largest dynamics of the Ebro River are concentrated along the meandering profile of the central sector. During the 20th century, this sector experienced a large alteration of its geomorphological structure. We present here an analysis of this evolution through the cartographic study of a long segment of the river (~250 km) in 1927, 1956 and 2003. The results show a large reduction in bank sinuosity, a progressive loss of fluvial territory, and a large decrease in channel width. These changes are especially clear in the areas previously most ecologically connected with the active channel. The fluvial territory of the river in 2003 was approximately half that found during the first decades of the 20th century. Forest plantations, which were non-existent in 1927, occupied more than 1500 ha of the study area in the last decade. This intense geomorphological transformation becomes ecologically visible in (i) a 35% reduction of the area occupied by riparian vegetation; (ii) a loss of the heterogeneity of riparian forest spots, which were formerly structured in an irregular mosaic far from the river thalweg; and (iii) a modification of the riparian forest structure, which is currently linear, uniform, thin and very close to the river axis. The ecomorphological alteration was intensified by the remarkable reduction in bank length (13%) and the reduced dynamism of the present river system, indicated by an increase in the percentage of fluvial territory occupied by riparian forests and a reduction in the area occupied by the active channel. Copyright © 2002 John Wiley & Sons, Ltd.
    Print ISSN: 0197-9337
    Electronic ISSN: 1096-9837
    Topics: Geography , Geosciences
    Published by Wiley
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  • 8
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    Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica (2013)
    Springer Nature
    Details
    Publication Date: 2013-07-25
    Description: Article Oleispira antarctica is an oil-degrading enzyme found in the cold and deep sea. Here Kube et al. report the genome sequence of O. antarctica and provide a comprehensive functional genetic and protein structural analysis, revealing insights into how this organism has adapted to its cold environment. Nature Communications doi: 10.1038/ncomms3156 Authors: Michael Kube, Tatyana N. Chernikova, Yamal Al-Ramahi, Ana Beloqui, Nieves Lopez-Cortez, María-Eugenia Guazzaroni, Hermann J. Heipieper, Sven Klages, Oleg R. Kotsyurbenko, Ines Langer, Taras Y. Nechitaylo, Heinrich Lünsdorf, Marisol Fernández, Silvia Juárez, Sergio Ciordia, Alexander Singer, Olga Kagan, Olga Egorova, Pierre Alain Petit, Peter Stogios, Youngchang Kim, Anatoli Tchigvintsev, Robert Flick, Renata Denaro, Maria Genovese, Juan P. Albar, Oleg N. Reva, Montserrat Martínez-Gomariz, Hai Tran, Manuel Ferrer, Alexei Savchenko, Alexander F. Yakunin, Michail M. Yakimov, Olga V. Golyshina, Richard Reinhardt, Peter N. Golyshin
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
    Published by Springer Nature
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  • 9
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    Feathers as a Biomonitoring Tool of Polyhalogenated Compounds: A Review. (2013)
    American Chemical Society (ACS)
    Details
    Publication Date: 2013-03-12
    Description: Environmental Science & Technology DOI: 10.1021/es302758x
    Print ISSN: 0013-936X
    Electronic ISSN: 1520-5851
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Published by American Chemical Society (ACS)
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  • 10
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    Correction to Feathers as a Biomonitoring Tool of Polyhalogenated Compounds: A Review (2013)
    American Chemical Society (ACS)
    Details
    Publication Date: 2013-08-09
    Description: Environmental Science & Technology DOI: 10.1021/es4033869
    Print ISSN: 0013-936X
    Electronic ISSN: 1520-5851
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Published by American Chemical Society (ACS)
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