ISSN:
1432-2048
Keywords:
Crown gall
;
Cytokinin (polypeptide regulation)
;
Habituation
;
Nicotiana (kinetin and protein synthesis)
;
Protein synthesis (cytokinin regulation)
;
Tissue culture (kinetin effect)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract When cloned pith and leaf tissues of Nicotiana tabacum L. cv. Havana 425 are subcultured for 3 d on auxin-containing medium and labelled for 18 h with [35S]methionine, up to 10% of the labelled, soluble-protein fraction is found in a single band with an apparent molecular weight of approx. 32,000–34,000 dalton on sodium-dodecylsulfate polyacrylamide-gel electrophoretograms. The labelling of this band, designated P33, is dramatically inhibited by the cytokinin, kinetin, in some cell lines at concentrations as low as 1.4·10-8 M. P33 is a major component of the protein fraction obtained from non-habituated clones, cytokinin-habituated clones, and revertant subclones of crown-gall-transformed clones, but cannot be detected in clones habituated for both auxin and cytokinin, or crown-gall-transformed clones. The evidence supports the hypothesis that cytokinin in the presence of auxin regulates the production of a specific, major polypeptide in the soluble-protein fraction of the tissue and that this protein is not produced in tissues autotrophic for both auxin and cytokinin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00397733
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