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  • 1
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Nodulated bean plants were exposed to mild salt stress or water deficit in such a way that the nodule's nitrogen-fixing activity was reduced to about 25–30% that of controls. Water-deprived plants showed a slight decrease in the weight of the aerial part, whereas the photosynthetic parameters were not significantly affected. In contrast, salt-stressed plants displayed a reversible decrease in the quantum yield of photosystem II photochemistry. Five water-deficit responsive cDNA clones encoding one lipid transfer protein, two late-embryogenesis abundant (LEA) proteins and two proline-rich proteins (PRPs) showed different organ-specific expression patterns depending on the kind of stress applied. PRPs and one LEA protein, PvLEA-18, exhibited the highest expression in nodules. Anti-PvLEA-18 antibodies were used to immunolocalize the protein in the nodule. PvLEA-18 was localized in the cytoplasm and nucleus of nodule cortex cells, and preferentially in cells of the vascular bundles, showing enhanced accumulation under water deficit. To our knowledge, this is the first time that a LEA protein has been identified in legume nodules.
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  • 2
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary. The effects of five different chemical groups of herbicides: dinitroanilines, 1, 3, 5-triazines, 1, 2, 4-triazinones, α-chloroacetamides and carbamates on N2-fixation & yield in Lupinus were compared. Cyanazine, simazine and trifluralin caused a significant increase in acetylene reduction activity (ARA), whereas the rest had no effect on ARA. Cyanazine and simazine significantly increased the grain yield and the weight of lupin seeds. The percentage of nitrogen in the seeds was significantly increased by cyanazine. An inventory of the weeds resistant to the herbicides in the treated subplots was also carried out. The dinitroaniline group mainly controlled Galium tricorne, Lolium multiflorum, Papaver rhoeas, and Avena sterilis whereas the 1, 3, 5-triazine and dinitroaniline herbicides. Carbamates and α-chloroacetamides were less effective Papaver rhoeas, Scandix pecten-veneris and Avena sterilis. Avena sterilis was controlled by both triazine and dinitro-aniline herbicides. Carbamates and α-chloroacetamides were less effective on the weeds growing with the lupinus plants.
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  • 3
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of isoproturon on the ultrastructure of the photosynthetic apparatus, ribulose bisphosphate carboxylase activity, protein and chlorophyll content, and the grain yield was investigated in two wheat cultivars (Triticum sativum L. cvs Castan and Esquilache) and a weed (Lolium rigidum Gaud.). Field experiments used applications of 1–65 and 2–5 kg a.i. ha−1 isoproturon post-emergence, and growth chamber experiments used nutrient solution with the addition of isoproturon (1·7 × 10−4 M). The ultrastructure of the photosynthetic apparatus of the cv. Esquilache was much affected by the herbicide. In the case of cv. Castan, slight disorganization of the grana and intergrana was observed. Isoproturon decreased the activity of ribulose bisphosphate carboxylase. A decrease in protein and chlorophyll content was also observed in the cv. Esquilache and in L. rigidum. These alterations were much less evident in the cv. Castan, where, moreover, no loss of protein occurred. The yield of the treated cv. Castan plants was slightly greater than that of the control plants in two consecutive years. However, the yields of the cv. Esquilache were significantly less when the herbicide was applied in the first year at commencement of tillering in a dry season but not when applied at an advanced stage as in the second year in a wet season.
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  • 4
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The genome of Phanerochaete chrysosporium strain ME446 contains multiple, non-allelic, cellobiohydrolase I (CSHI)-like sequences, at least two of which are expressed in a cellulose-dependent manner. Each of the expressed genes contains two identically positioned introns within its coding region. The lengths and sequences of these introns are different and one is not excised from all transcripts, raising the possibility that subtly different protein products may be expressed from a common gene. Introns are also present upstream of both genes but these differ in number and position, as well as sequence and length. Endoglucanase-like sequences could not be identified and it is suggested that variant CBHI-like proteins may provide endoglucanase activity in this fungus.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 89 (1993), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Oxygen shortage in soils can occur following a wide range of natural circumstances, affecting the plant's physiology. In this paper the performance of nodulated lucerne plants under severe hypoxia is examined and the mechanisms involved to achieve this adaptation are discussed. Nodulated lucerne plants (Medicago sativa L.) were grown with their rooting medium exposed to 1 or 21 kPa oxygen. Final yield, as expressed on a shoot dry weight basis, was unaffected but root and nodule dry weights were reduced by 50%. Water content in roots and nodules was higher at 1 kPa as a result of the formation of aerenchyma. Specific acetylene reduction activity was higher in hypoxic nodules as a consequence of modified nodule structure, although they were more sensitive to the presence of acetylene or nitrate. Root respiration was insensitive to changes in external oxygen supply, therefore providing adequate support for mineral uptake. Nodule respiration rates were 5 times higher in control plants when measured as CO2 evolution, whereas no differences were observed in O2 uptake. It is suggested that adaptation of nodulated lucerne to low oxygen concentrations involves changes in photosynthate allocation and nodule morphology, which provide a more efficient nitrogen fixation.
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  • 6
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of isoproturon [N,N-dimethyl N′4-(1-methyl-ethyl) phenyl urea] on the germination, growth of root and ultrastructure of the photosynthetic apparatus was investigated in two wheat cultivars (Triticum sativum L. cvs Castan and Esquilache) and a weed (Lolium rigidum Gaud.). No inhibition of germination was apparent in any of the three plants studied. The inhibition of root growth was considerably significant, especially in cv. Esquilache. After 48 h treatment with isoproturon (340 μM), the chloroplasts of the two wheat cultivars were found to be similar, showing a higher number of grana with fewer thylakoids per granum (lower stacking degree), broader grana stacks than in the control and an absence of starch. However, after 60 h of treatment, swelling of the thylakoids occurred in the Esquilache cultivar.The rye grass was the most affected by the herbicide; at low concentration (3.4 μM) the number and size of the grana decreased considerably and the stroma thylakoids were destroyed. At higher concentration (17 μM), there was a strong accumulation in certain areas of the stroma of a substance with a lipid-like appearance, probably derived from the disintegrating thylakoids. At the same concentrations some chloroplast envelopes were completely desintegrated.The Hill reaction of the three plants exhibited a similar sensitivity towards the herbicide.
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  • 7
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fifty isolates of Crinipellis perniciosa originating from Theobroma cacao, Heteropterys acutifolia and Solanum lycocarpum, from six states within Brazil, were characterized through ERIC-PCR, representing the first application of this method for molecular characterization within C. perniciosa. Phenetic analysis of banding patterns revealed a separation of isolates on the basis of host of origin, with T. cacao-derived isolates showing only a 0·2 similarity level to a cluster comprising the isolates from H. acutifolia and S. lycocarpum. Considerable intraspecific variability was observed within C. perniciosa isolates from T. cacao, with distinct groups observed correlating with geographical origin. Given that a number of isolates from T. cacao from the Amazon region grouped with isolates from Bahia state, this work discusses the possibility that current C. perniciosa populations pathogenic on T. cacao in Bahia originated from the Amazon region, rather than from alternative host plants.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 56 (1982), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of different nitrogen sources (ammonium, nitrate or both ions together) on plastid development in dark-grown and illuminated seedlings of wheat (Triticum vulgare L. cv. Yecora) has been investigated. Plastids of plants grown in ammonium showed even in the dark a larger internal membrane length, higher ribulose bisphos-phate carboxylase activity and greater content of soluble proteins than plastids of plants grown in nitrate. After the first hour of illumination rudimentary thylakoids showing some joining points were observed in the ammonium plastids. After 10 h no prolamellar bodies were seen in the ammonium plastids, and the internal plastid membrane length was greater than in the other treatments. There was no light-induced increase in protein synthesis after illumination for 1 h. After 10 h the increase observed in protein synthesis was not followed by a response in the enzyme activity in any of the treatments. After 20 h the lag in the induction of ribulose bisphosphate carboxylase ceased, the enzyme activity and soluble proteins being higher in the leaves of ammonium seedlings than in those from nitrate. From the correlation obtained between the ultrastructural electron microscope observations and the enzymatic studies, it appears that ammonium nutrition has a positive influence on the formation of the plastid membrane system and on the onset of photosynthesis and, consequently, on the development of chloroplasts.
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  • 9
    ISSN: 1615-6102
    Keywords: Lupinus albus ; Nitrogen fixation ; Oxygen diffusion ; Glycoprotein ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The monoclonal antibodies MAC236 and MAC265, raised against a soluble component of pea nodules, were used to elucidate the presence and subcellular localization of glycoprotein epitopes during the development of lupin (Lupinus albus L. cv. Multolupa) nodules, by means of immunocytochemistry and Western blot analysis. These antibodies recognize a single band of 95 kDa in pea, soybean and bean nodules, whilst two different bands of 240 and 135 kDa cross-react with MAC236 and MAC265 respectively in lupin nodules. This fact may indicate that the recognized epitopes can be present in different subcellular compartments and/or play different roles through the development of functional nodules. The results show that MAC265 is mainly associated with Bradyrhizobium infection and with the development of nodule primordium, in the first stages of nodulation. MAC265 is also detected when glycoprotein transport takes place across the cytoplasm and the cell wall, and also in the intercellular spaces of the middle cortex, attached to cell walls. The amount of MAC265 remains constant through nodule development. In contrast the amount of MAC236 increases with nodule age, parallel to the establishment of nitrogenase activity. This antibody is localized in cytoplasmic globules attached to the inner side of cell walls in the middle cortex, and mainly in the matrix filling the intercellular spaces of the middle and inner cortex. This main site of localization of MAC236 may indicate a role in the functioning of the oxygen diffusion barrier.
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  • 10
    ISSN: 1615-6102
    Keywords: Ferritin ; Nitrogen fixation ; Nodule development ; Plastid ; Legume ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In eukaryotic organisms ferritin is a protein involved in the storage of iron. The occurrence of ferritin and its relationship to the effectiveness of the nitrogen-fixing activity have been previously studied during the early stages of the nodule development by biochemical methods. We have used immunocytochemistry techniques to determine the precise location of ferritin and the behavior of this protein along the nodule development. The major localization was found in plastids and amyloplasts of infected and uninfected cells of the three legume nodules studied. A decrease of the immunolabelling was observed in infected cells of lupin and soybean senescing nodules and in the senescent zone of indeterminate alfalfa nodules. In the cortex of soybean and lupin nodules, ferritin increased during nodule ageing and the immunogold particles were mainly located in crystalline structures. The putative role of ferritin and plastids during nodule development is discussed.
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